重组小鼠SMAD5蛋白

小鼠

杆状病毒 - 昆虫细胞

Thr 2 - Ser 465

A DNA sequence encoding the mouse SMAD5 (P97454) (Thr2-Ser465) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

> 95 % as determined by SDS-PAGE

The recombinant mouse SMAD5/GST chimera consists of 701 amino acids and has a calculated molecular mass of 79.9 kDa. The recombinant protein migrates as an approximately 75-88 kDa band in SDS-PAGE under reducing conditions.

MOTHERS AGAINST DECAPENTAPLEGIC HOMOLOG 5; DWARFIN-C; 1110051M15RIK; MUSMLP; MAD; MAD HOMOLOG 5; MADH5; SMAD FAMILY MEMBER 5; HOMOLOG OF; MOTHERS AGAINST DECAPENTAPLEGIC; DWF-C; MOTHERS AGAINST DPP HOMOLOG 5; SMAD; SMAD5; SMA- AND MAD-RELATED PROTEIN 5; DWFC; AI451355; DROSOPHILA; JV5-1; MSMAD5; SMAD 5

Mothers against decapentaplegic homolog 5

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.