重组小鼠EPOR蛋白

小鼠

杆状病毒 - 昆虫细胞

Met 1 - Pro 249

A DNA sequence encoding the mouse EPOR (NP_034279.3) (Met1-Pro249) was expressed, fused with the Fc region of human IgG1 at the C-terminus.

> 85 % as determined by SDS-PAGE

1. Measured by its ability to inhibit EPOdependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.05-0.2 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.
2. Measured by its binding ability in a functional ELISA.
3. Immobilized mouse EPO-His (Cat:504106) at 10μg/mL (100 μL/well) can bind mouse EPOR-Fc. The EC₅₀ of mouse EPOR-Fc is 0.06-0.13μg/mL.

The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.

EPO R; EPOR; EPO RECEPTOR; ERYTHROPOIETIN RECEPTOR; EPO-R

Erythropoietin receptor

Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â…  single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.

Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.