- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IF, WB, IP, FC
- 特异性:Human, Mouse; other species not tested.
产品详情
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产品名称
Mitofilin antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Mitofilin Rabbit Polyclonal antibody. Positive FC detected in HEK-293T cells. Positive IHC detected in human gliomas tissue, human breast cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in multi-cells/tissue, COLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, human brain tissue, MCF7 cells, Raji cells. Positive IP detected in Raji cells. Observed molecular weight by Western-blot: 87 kDa,89 kDa
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抗体应用
ELISA, IHC, IF, WB, IP, FC
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human, Mouse; other species not tested.
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蛋白别名
DKFZp779P1653, HMP, IMMT, MIC60, MINOS2, Mitofilin, P87, P87/89, P89, PIG4, PIG52
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制备方法
This antibody was obtained by immunization of Mitofilin recombinant protein (Accession Number: XM_017003194). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
IMMT (also known as mitofilin) is an inner mitochondrial membrane protein that is preferentially expressed in heart tissue and is commonly used as the marker for mitochondria. Mitofilin is known to be a critical organizer of mitochondrial cristae morphology and is indispensable for normal mitochondrial function. Three isoforms of mitofilin exist due to the alternative splicing. All three proteins of 87kD, 89kD and 80kD can be detected in immunoblot analysis with this antibody.
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参考文献
- Ding C, Wu Z, Huang L. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Scientific reports. 5:16064. 2015.
- Genin EC, Plutino M, Bannwarth S. CHCHD10 mutations promote loss of mitochondrial cristae junctions with impaired mitochondrial genome maintenance and inhibition of apoptosis. EMBO molecular medicine. 8(1):58-72. 2015.
- Mateo R, Nagamine CM, Kirkegaard K. Suppression of Drug Resistance in Dengue Virus. mBio. 6(6):e01960-15. 2015.
- Yin X, Mayr M, Xiao Q, Wang W, Xu Q. Proteomic analysis reveals higher demand for antioxidant protection in embryonic stem cell-derived smooth muscle cells. Proteomics. 6(24):6437-46. 2006.
- Weihofen A, Thomas KJ, Ostaszewski BL, Cookson MR, Selkoe DJ. Pink1 forms a multiprotein complex with Miro and Milton, linking Pink1 function to mitochondrial trafficking. Biochemistry. 48(9):2045-52. 2009.
- Jiang L, Fang J, Moore DS. Age-associated changes in synaptic lipid raft proteins revealed by two-dimensional fluorescence difference gel electrophoresis. Neurobiology of aging. 31(12):2146-59. 2010.
- An J, Shi J, He Q. CHCM1/CHCHD6, novel mitochondrial protein linked to regulation of mitofilin and mitochondrial cristae morphology. The Journal of biological chemistry. 287(10):7411-26. 2012.
- Anand R, Wai T, Baker MJ. The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. The Journal of cell biology. 204(6):919-29. 2014.