重组MERS冠状病毒Spike蛋白

MERS冠状病毒

杆状病毒 - 昆虫细胞

Glu 367 - Tyr 606

A DNA sequence encoding the spike protein fragment (Human betacoronavirus 2c EMC/2012)(AFS88936.1) (Glu367-Tyr606) was fused with the Fc region of rabbit IgG at the C-terminus.

> 80 % as determined by SDS-PAGE

The recombinant spike protein fragment (Human betacoronavirus 2c EMC/2012) comprises 46.4 amino acids and has a predicted molecular mass of 51.5 kDa. It migrates as an approximately 54.3 kDa band in SDS-PAGE under reducing conditions.

S protein

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell: they are essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. Most notable is severe acute respiratory syndrome (SARS). The severe acute respiratory syndrome-coronavirus (SARS-CoV) spike (S) glycoprotein alone can mediate the membrane fusion required for virus entry and cell fusion. It is also a major immunogen and a target for entry inhibitors. The SARS-CoV spike (S) protein is composed of two subunits; the S1 subunit contains a receptor-binding domain that engages with the host cell receptor angiotensin-converting enzyme 2 and the S2 subunit mediates fusion between the viral and host cell membranes. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity, during infection with SARS-CoV. 

Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.2.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.