重组人LEP蛋白

人

大肠杆菌

Val 22 - Cys 167

A DNA sequence encoding the mature form of human Leptin (NP_000221.1) (Val 22-Cys 167) was expressed, with an additional Met.

> 98 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
1. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor Fc chimera with a linear range of 0.032-4.0 μg/ml.
2. Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor his with a linear range of 0.032-4.0 μg/ml.

The recombinant human leptin consists of 147 amino acids and predicts a molecular mass of 16 kDa. The apparent molecular mass of rhLEP is approximately 13 kDa in SDS-PAGE under reducing conditions.

OB; LEPTIN; LEP; OBS; OBESE; LEPD; FLJ94114

Leptin

May function as part of a signaling pathway that acts to regulate the size of the body fat depot.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.