产品详情
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产品名称
重组人K-Ras4B蛋白
- 文档
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蛋白种属
人
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表达宿主
大肠杆菌
- Unitprot ID
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蛋白序列
MNHKVHHHHHHENLYFQSMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVKDSEDVPMVLVGNKSDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREIRKHKEK
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克隆构建
DNA encoding 1‑169 aa (P01116‑2, C118S) was fused with a 6‑His tag.
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蛋白纯度
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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蛋白活性
Not tested.
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分子量
Predictes a molecular mass of 21.53 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 23.7 kDa band.
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蛋白全名
GTPase KRas
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蛋白描述
This product is a soluble recombinant human KRAS4b (C118S mutant) protein (amino acid 1-169) with an N-terminal 6×His tag. The protein is produced via Escherichia coli high-efficiency expression and purification, with outstanding solubility and high purity. The C118S point mutation is constructed on the basis of native KRAS4b 1-169aa sequence, suitable for KRAS post-translational modification research, protein stability analysis and tumor pathogenesis study.
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检测结果
Recombinant Human KRAS (C118S) Protein SDS-PAGE
M: Marker; Lane 1: KRAS (C118S); Lane 2: BSA
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发货形式
Lyophilized from sterile 25 mM HEPES, 150 mM NaCl, pH 7.4
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内毒素
Not tested.
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保存条件
Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.