重组人IL4R蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - His 232

A DNA sequence encoding the human IL4R (NP_000409.1) precursor (Met 1-His 232) was fused with a polyhistidine tag at the C-terminus.

> 98 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human IL4R-His (Cat:502315) at 10 μg/mL (100 μl/well) can bind biotinylated human IL4 (Cat:500224). The EC₅₀ of biotinylated human IL4 is 20.8-48.5 ng/mL.
2. Measured by its ability to inhibit IL4 dependent proliferation of TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 1-4 ng/ml in the presence of 0.2 ng/ml Recombinant Human IL4.

The secreted recombinant human IL4R consists of 218 amino acids after cleavage of the signal peptide and predictes a molecular mass of 25.3 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 43-48 kDa due to glycosylation.

IL4R; MGC118473; CD124; IL4RA

Interleukin-4 receptor subunit alpha

Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.