重组人IL33蛋白

人

大肠杆菌

Ser 112 - Thr 270

A DNA sequence encoding the C-terminal segment of human IL33 (NP_254274.1) (Ser 112-Thr 270) was expressed with an initial Met at the N-terminus.

> 98 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA. Immobilized human IL-33 at 10 μg/ml (100 μl/well) can bind human IL1RL1 with a linear range of 0.31-5 ng/ml.
2. Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells costimulated with antiCD3. The ED₅₀ for this effect is typically 0.15-0.6 ng/mL.

The recombinant human IL33 comprises 160 amino acids and predicts a molecular mass of 18 kDa as estimated in SDS-PAGE under reducing conditions.

DKFZP586H0523; IL-33; C9ORF26; 9230117N10RIK; DVS27; IL33; NF-HEV; RP11-575C20.2; NFEHEV; IL1F11

Interleukin-33

Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines. Also involved in activation of mast cells, basophils, eosinophils and natural killer cells. Acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528).

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.