重组人PGA4蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Ala 388

A DNA sequence encoding the human PGA4 (P00790) (Met 1-Ala 388) was fused with a polyhistidine tag at the C-terminus.

> 97 % as determined by SDS-PAGE

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2, AnaSpec, Catalog # 27114.
The specific activity is > 10,000 pmoles/min/μg.

The secreted recombinant human PGA4 consists of 384 amino acids and has a predicted molecular mass of 41.7 kDa. It migrates as an approximately 45 kDa band in SDS-PAGE under reducing conditions.

PEPSINOGEN A; PGA5; FLJ58952; FLJ77962; PGA4; PGA3

PGA4 (Pepsinogen 4, group I), or Pepsinogen A, is a member of the peptidase A1 family. Pepsin is expressed as a pro-form zymogen, pepsinogen, whose primary structure has an additional 44 amino acids. Pepsin is stored as pepsinogen so it will only be released when needed, and does not digest the body's own proteins in the stomach's lining. Five types of zymogens of pepsins, gastric digestive proteinases, are known: pepsinogens A, B, and F, progastricsin, and prochymosin. There are two major groups of pepsinogen, namely pepsinogen A (PGA) and pepsinogen C (PGC) (or progastricsin), and each frequently has isozymogens. The PGA3, PGA4 and PGA5 genes encode identical human pepsinogen A enzymes.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.