重组人LYPLA1蛋白

人

大肠杆菌

A DNA sequence encoding the Human LYPLA1/APT1/LPL1 Met1-Asp230 is expressed with a 6His tag at the N-terminus.

>95% as determined by SDS-PAGE.

APT1; LPL1

lysophospholipase I(LYPLA1)

Acyl-Protein Thioesterase 1 (APT-1) is lysophospholipase that belongs to the AB hydrolase 2 family. APT-1 performs on biological membranes to regulate the multifunctional lysophospholipids. It hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS; in addition, it also has depalmitoylating activity and low lysophospholipase activity.

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 100mM NaCl, 1mM DTT, 10% Glycerol, pH 8.0.

<1.0 EU per μg protein as determined by the LAL method.

Store at <-20°C, minimize freeze-thaw cycles.