重组人LEPR蛋白

人

人胚胎肾细胞（HEK293）

Met 1 - Asp 839

A DNA sequence encoding the extracellular domain (Met 1-Asp 839) of human leptin receptor precursor (NP_002294.2) was expressed with the C-terminal fused polyhistidine-tagged Fc region of human IgG1.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/ml.

The recombinant human LEPR/Fc is a disulfide-linked homodimeric protein.The reduced monomer consists of 1065 amino acids and has a predicted molecular mass of 121.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR/Fc monomer is approximately 155-165 kDa due to glycosylation.

MODB1; RP23-31K16.2; DIABETES; DB; LEPR; MGC105189; CD295; OB-RGRP; OB-R; OBL; LEP-R; LEPRB; LEPROT; OBR; OBESE-LIKE; LEPRD

Leptin receptor

Leptin Receptor or CD295 belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Leptin Receptor/CD295 is a transmembrane catalytic receptors found on NPY/AgRP and alpha-MSH/CART neurons in hypothalamic nuclei. Leptin receptors (Ob-Rs) are coded for by one human gene that produces six different isoforms; Ob-Ra - Ob-Rf. Ob-Rs exist as constitutive dimers at physiological expression levels. Only the Ob-Rb isoform can transduce intracellular signals and does so through activation of the JAK2/STAT3, PI 3-K and MAPK signaling cascades. Activation of Ob-Rs mediates transcriptional regulation of the hypothalamic melanocortin pathway and downregulates endocannabinoid expression. Leptin acts via leptin receptors. Leptin resistance has been proposed as a pathophysiological mechanism of obesity. In obese individuals, Ob-Ra (which is involved in active transport of leptin across the blood-brain barrier) expression is downregulated and the individual may be unresponsive to leptin signals. Ob-R antagonists are of great interest in the development of pharmacological treatments for obesity. Mutations in Leptin Receptor/CD295 have been associated with obesity and pituitary dysfunction.

Lyophilized from sterile PBS, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.