重组人VTCN1蛋白

人

人胚胎肾细胞（HEK293）

Phe 29 - Ala 258

A DNA sequence encoding the human VTCN1(Q7Z7D3) (Phe29-Ala258) with a C-terminal polyhistidine tag was expressed.

> 95 % as determined by SDS-PAGE

The recombinant human VTCN1 comprises 241 amino acids and has a predicted molecular mass of 26.8 kDa. The apparent molecular mass of the protein is approximately 43-48 kDa in SDS-PAGE under reducing conditions.

IMMUNE COSTIMULATORY PROTEIN B7-H4; B7H4; T CELL COSTIMULATORY MOLECULE B7X; B7S1; BC032925; V-SET DOMAIN CONTAINING T-CELL ACTIVATION INHIBITOR 1; B7X

V-set domain-containing T-cell activation inhibitor 1

V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type? membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.

Lyophilized from sterile PBS, pH 7.4.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.