重组人ATG5蛋白

人

大肠杆菌

A DNA sequence encoding the Human ATG5/APG5L/ASP Met1-Asp275 is expressed.

>95% as determined by SDS-PAGE.

APG5L; ASP

autophagy related 5(ATG5)

ATG5 is an E2 ubiquitin ligase which is necessary for autophagy. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity, dramatically highly expressed in apoptotic cells. It is activated by ATG7, conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate complex detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 plays an important role in the apoptotic process, possibly within the modified cytoskeleton.

Supplied as a 0.2 μm filtered solution of 20mM Tris,0.2M NaCl,1mM DTT,40%glycerol, pH 8.0 .

<1.0 EU per μg protein as determined by the LAL method.

Store at <-20°C, minimize freeze-thaw cycles.