重组人YWHAE蛋白

人

大肠杆菌

Met 1 - Gln 255

A DNA sequence encoding the human YWHAE (NP_006752.1) (Met 1-Gln 255) was fused with the GST tag at the N-terminus.

> 92 % as determined by SDS-PAGE

The recombinant human YWHAE/GST chimera consists of 487 amino acids and has a predicted molecular mass of 56 kDa. It migrates as an approxiamtely 50 kDa band in SDS-PAGE under reducing conditions.

MDCR; KCIP-1; 14-3-3E; FLJ45465; MDS; HEL2; 14-3-3 EPSILON; FLJ53559; YWHAE; AU019196; RP23-78H4.1

14-3-3 protein epsilon

YWHAE, also known as 14-3-3 epsilon, mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3 epsilon / YWHAE is a member of the 14-3-3 proteins family. 14-3-3 proteins are a group of highly conserved proteins that are involved in many vital cellular processes such as metabolism, protein trafficking, signal transduction, apoptosis and cell cycle regulation. 14-3-3 proteins are mainly localized in the synapses and neuronal cytoplasm, and seven isoforms have been identified in mammals. This family of proteins was initially identified as adaptor proteins which bind to phosphoserine-containing motifs. Binding motifs and potential functions of 14-3-3 proteins are now recognized to have a wide range of functional relevance. 14-3-3 epsilon / YWHAE is found in both plants and mammals, and this protein is 100% identical to the mouse ortholog. YWHAE interacts with CDC25 phosphatases, RAF1 and IRS1 proteins, suggesting its role in diverse biochemical activities related to signal transduction, such as cell division and regulation of insulin sensitivity. It has also been implicated in the pathogenesis of small cell lung cancer. 14-3-3 epsilon / YWHAE is implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 epsilon / YWHAE Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. This Binding generally results in the modulation of the activity of the binding partner.

Lyophilized from sterile 20mM Tris, 150mM NaCl, 0.25mM DTT, 25% glycerol, 0.5mM GSH, pH 7.5
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.