重组人蛋白

人

大肠杆菌

DNA encoding 1-182 aa were fused with N-terminal GST tag.

>95%, by SDS-PAGE with Coomassie Brilliant Blue staining.

5U/μL. One unit (1 U) is defined as the amount of enzyme required to cleave 95% of 100 μg fusion protein in 25 mM Tris‑HCl buffer (pH 8.0) within 12–16 h at 4 °C.

Predictes a molecular mass of 46.28 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 46 kDa band.

Human Rhinovirus 3C Protease

HRV 3C Protease (Human Rhinovirus 3C Protease) refers to the 3C protease of Human Rhinovirus (HRV), which recognizes and cleaves the specific amino‑acid sequence: Leu‑Glu‑Val‑Leu‑Phe‑Gln↓Gly‑Pro (where "↓" indicates the cleavage site). This recombinant HRV‑3C protease has a molecular weight of approximately 46 kDa and carries an N‑terminal GST tag. HRV 3C protease exhibits activity across a wide range of pH values, ionic strengths and temperatures. It retains high activity even at 0 °C, making it an ideal choice for temperature‑sensitive proteins.

Sterile liquid solution contains 25 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 50% Glycerol, pH 8.0.

Please contact the lab for more information.

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.