- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IF, FC, WB
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
HIF1a antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
HIF1a Rabbit Polyclonal antibody. Positive WB detected in Cobalt Chloride treated HeLa cells, HeLa cells, mouse small intestine tissue. Positive IF detected in Hela cells. Positive IHC detected in human thyroid cancer tissue, human heart tissue, human kidney tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 120 kDa
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抗体应用
ELISA, IHC, IF, FC, WB
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应用推荐
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
ARNT interacting protein, bHLHe78, HIF 1 alpha, HIF 1alpha, HIF1, HIF1 ALPHA, HIF1A, Member of PAS protein 1, MOP1, PASD8
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制备方法
This antibody was obtained by immunization of HIF1a recombinant protein (Accession Number: NM_001530). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
HIF1, the major regulator of the cellular responses to hypoxia, consists of an oxygen-sensitive subunit, HIF1alpha (HIFA), and an oxygen-insensitive subunit, HIF1beta (arylhydrocarbon receptor nuclear transporter [ARNT]). Although the mRNA is constitutively and ubiquitously expressed and transcribed, the HIF1A protein is rapidly degraded in the presence of oxygen. The half-life of HIF1A under ambient oxygeconditions is less than 5 min. Both hypoxic conditions and chemical hydroxylase inhibitors (such as desferrioxamine and cobalt) inhibit HIF-1α degradation and lead to its stabilization. In addition, HIF-1α can be induced in an oxygen-independent manner by various cytokines through the PI3K-AKT-mTOR pathway.
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参考文献
- Lin J, Qin X, Zhu Z. FHL family members suppress vascular endothelial growth factor expression through blockade of dimerization of HIF1α and HIF1β. IUBMB life. 64(11):921-30. 2012.
- Qu K, Yan Z, Wu Y. Transarterial chemoembolization aggravated peritumoral fibrosis via hypoxia-inducible factor-1α dependent pathway in hepatocellular carcinoma. Journal of gastroenterology and hepatology. 30(5):925-32. 2015.
- Hu T, He N, Yang Y, Yin C, Sang N, Yang Q. DEC2 expression is positively correlated with HIF-1 activation and the invasiveness of human osteosarcomas. Journal of experimental & clinical cancer research : CR. 34:22. 2015.
- Chen Z, Zhang Y, Jia C. mTORC1/2 targeted by n-3 polyunsaturated fatty acids in the prevention of mammary tumorigenesis and tumor progression. Oncogene. 33(37):4548-57. 2014.
- Peng B, Liu F, Han R. Dynamic metabolic change is indicative of inflammation-induced transformation of hepatic cells. The international journal of biochemistry & cell biology. 66:45-58. 2015.
- Liu F, Wang B, Li L. Low-Dose Cadmium Upregulates VEGF Expression in Lung Adenocarcinoma Cells. International journal of environmental research and public health. 12(9):10508-21. 2015.
- Liu F, Zhang W, You X. The oncoprotein HBXIP promotes glucose metabolism reprogramming via downregulating SCO2 and PDHA1 in breast cancer. Oncotarget. 6(29):27199-213. 2015.
- Shan X, Wang D, Chen J. Necrosis degree displayed in computed tomography images correlated with hypoxia and angiogenesis in breast cancer. Journal of computer assisted tomography. 37(1):22-8. 2013.