HAVDI 多肽

Fmoc-HAVDI-OH

Fmoc-His-Ala-Val-Asp-Ile-OH

5

99.46

C₃₉H₄₉N₇O₁₀

775.84

固相合成

HAVDI polypeptide, a synthetic peptide engineered to mimic the functional properties of the N - cadherin protein, is designed with a precise sequence to actively interact with cells and modulate their behavior. This peptide exhibits the remarkable ability to self-assemble into nanofibrous structures, thereby creating a three-dimensional scaffold that bears a striking resemblance to the natural extracellular matrix (ECM). This scaffold not only offers essential physical support to cells but also plays a crucial role in guiding their growth, adhesion, and proliferation processes.
The specifically designed N-cadherin mimetic peptides, namely Fmoc-HAVDI and Nap-HAVDI, exemplify this self-assembly property. At physiological pH, they spontaneously form a nanofibrous network, which gives rise to a bioactive peptide hydrogel. The nanofibrous network within these pentapeptide hydrogels closely mimics the topology of the natural ECM, and interestingly, the mechanical strength of the gels precisely matches that of the native ECM of neural cells. This unique combination of properties enables the hydrogels to effectively support cell adhesion and proliferation of both neural and non-neural cell lines, thus highlighting the versatility and adaptability of these peptidic scaffolds.
In the realm of tissue engineering, HAVDI polypeptide has emerged as a highly promising candidate. It actively promotes cell-cell interactions, a fundamental process for tissue regeneration. Its biocompatibility with a diverse range of cell types, including both neural and non-neural cells, is a significant advantage, ensuring minimal cytotoxicity and maximizing its potential for use in vivo. This peptide's distinctive characteristics render it an invaluable tool for in-depth studies of cell behavior and for the development of novel, innovative treatment in the field of regenerative. By leveraging its properties, it becomes possible to engineer functional tissues and organs, opening up new avenues and possibilities for the treatment of a wide spectrum of diseases and injuries. Moreover, the tunable nature of the HAVDI polypeptide allows for customization to meet the specific requirements of different applications, further augmenting its versatility and effectiveness in various biomedical contexts.

一般情况下， HAVDI 多肽 粉末应该保存在-20甚至-70摄氏度. HAVDI 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Kaur H, Roy S. Designing aromatic N-cadherin mimetic short-peptide-based bioactive scaffolds for controlling cellular behaviour. J Mater Chem B. 2021 Aug 7;9(29):5898-5913. doi: 10.1039/d1tb00598g. Epub 2021 Jul 15. PMID: 34263278.

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。