重组小鼠GREM1蛋白

小鼠

杆状病毒 - 昆虫细胞

Met 1 - Asp 184

A DNA sequence encoding the mouse GREM1 (O70326) (Met1-Asp184) was fused with a polyhistidine tag at the C-terminus.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells.
The ED₅₀ for this effect is typically 1-7 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.

The recombinant mouse GREM1 consists of 171 amino acids and has a calculated molecular mass of 19.7 kDa. The recombinant protein migrates as an approximately 25 kDa band in SDS-PAGE under reducing conditions.

PROLIFERATION-INDUCING GENE 2; BMP ANTAGONIST 1; DRM; INCREASED IN HIGH GLUCOSE-2; LD; IHG-2; CYSTEINE KNOT SUPERFAMILY 1; CYSTEINE KNOT SUPERFAMILY; GREMLIN 1-LIKE PROTEIN; CRAC1; GREMLIN 1; GREMLIN; DAND2; DOWN-REGULATED IN MOS-TRANSFORMED CELLS PROTEIN; HMPS; C15DUPQ; DOWN-REGULATED IN V-MOS-TRANSFORMED CELLS; DUP15Q; GREMLIN-1; CRCS4; RP23-178K16.1; HMPS1; GREM; CKTSF1B1; DAN DOMAIN FAMILY MEMBER 2; PIG2; INCREASED IN HIGH GLUCOSE PROTEIN 2; CELL PROLIFERATION-INDUCING GENE 2 PROTEIN; MPSH; MGC126660; HOMOLOG

Gremlin-1

Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly, 0.5mM EDTA, 3mM DTT
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.