- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC, IP, WB, IF
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
FAK antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
FAK Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in MCF7 cells, A431 cells, HEK-293 cells, HeLa cells, Jurkat cells, NIH/3T3 cells. Positive IHC detected in human gliomas tissue. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 110 kDa
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抗体应用
ELISA, IHC, IP, WB, IF
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
FADK, FADK 1, FAK, FAK1, FOCal adhesion kinase 1, pp125FAK, Protein tyrosine kinase 2, PTK2, PTK2 protein tyrosine kinase 2
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制备方法
This antibody was obtained by immunization of FAK recombinant protein (Accession Number: BC028733). Purification method: Antigen affinity purified.
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组分
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has 6 isoforms produced by alternative promoter usage and alternative splicing.
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参考文献
- Lin CL, Zhang ZX, Xu YJ, Ni W, Chen SX. Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis. Chinese medical journal. 118(1):20-6. 2005.
- Ding J, Huang S, Wu S. Gain of miR-151 on chromosome 8q24.3 facilitates tumour cell migration and spreading through downregulating RhoGDIA. Nature cell biology. 12(4):390-9. 2010.
- Zhang H, Chen X, Liu B, Zhou L. Effects of stable knockdown of Aurora kinase A on proliferation, migration, chromosomal instability, and expression of focal adhesion kinase and matrix metalloproteinase-2 in HEp-2 cells. Molecular and cellular biochemistry. 357(1-2):95-106. 2011.
- Zeng F, Luo F, Lv S. A monoclonal antibody targeting neuropilin-1 inhibits adhesion of MCF7 breast cancer cells to fibronectin by suppressing the FAK/p130cas signaling pathway. Anti-cancer drugs. 25(6):663-72. 2014.
- Liu C, Liu Y, Xu XX. Potential effect of matrix stiffness on the enrichment of tumor initiating cells under three-dimensional culture conditions. Experimental cell research. 330(1):123-34. 2015.
- Wu Q, He J, Mei W, Zhang Z, Wu X, Sun F. Arene ruthenium(ii) complex, a potent inhibitor against proliferation, migration and invasion of breast cancer cells, reduces stress fibers, focal adhesions and invadopodia. Metallomics : integrated biometal science. 6(12):2204-12. 2014.
- Huang W, Liu J, Feng X. DLC-1 induces mitochondrial apoptosis and epithelial mesenchymal transition arrest in nasopharyngeal carcinoma by targeting EGFR/Akt/NF-κB pathway. Medical oncology (Northwood, London, England). 32(4):115. 2015.