重组大鼠EPO蛋白

大鼠

杆状病毒 - 昆虫细胞

Met 1 - Arg 192

A DNA sequence encoding the rat EPO (P29676-1) (Met 1-Arg 192) was fused with a polyhistidine tag at the C-terminus.

> 90 % as determined by SDS-PAGE

1. Measured by its ability to bind human EPOR-Fc (Cat:504113) in a functional ELISA.
2. Measured by its ability to bind biotinylated human EPOR-His (Cat:502594) in a functional ELISA.
3. Measured by its ability to bind mouse EPOR-Fch (Cat:50031-M03H) in a functional ELISA.
4. Measured by its ability to bind human EPOR & CD131-Fc (Cat:CT010-H02H) in a functional ELISA.
5. Measured by its ability to inhibit Epodependent proliferation of TF1 human erythroleukemic cells.
The ED₅₀ for this effect is 2-10ng/mL.

The recombinant rat EPO comprises 176 amino acids with and has a predicted molecular mass of 20 kDa. It migrates as an approximately 27 kDa band in reduced SDS-PAGE.

OTTMUSP00000024625; EPO; OTTMUSP00000024626; MGC138142; ERYTHROPOIETIN; MVCD2; EP; OTTMUSP00000024624

Erythropoietin

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

< 1.0 EU per μg of the protein as determined by the LAL method

Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.