- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC, IF
- 特异性:Human,Mouse,Rat; other species not tested.
产品详情
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产品名称
ERI1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
ERI1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue, human liver cancer tissue. Positive IF detected in HepG2 cells. Positive WB detected in K-562 cells, HepG2 cells, HL-60 cells. Observed molecular weight by Western-blot: 46kd
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抗体应用
ELISA, WB, IHC, IF
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应用推荐
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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特异性
Human,Mouse,Rat; other species not tested.
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蛋白别名
3 5 exonuclease ERI1, 3 5 exoribonuclease 1, 3EXO, 3'HEXO, Eri 1 homolog, ERI1, exoribonuclease 1, HEXO, Histone mRNA 3 exonuclease 1, Protein 3hExo, THEX1
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制备方法
This antibody was obtained by immunization of ERI1 recombinant protein (Accession Number: NM_153332). Purification method: Antigen affinity purified.
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组分
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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储存方法
Store at -20℃. DO NOT ALIQUOT
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背景介绍
ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.