重组大肠杆菌lacZ蛋白

大肠杆菌

大肠杆菌

A DNA sequence encoding the E.coli lacZ/b0344/JW0335 Met1-Lys1024(12-41AA deletion) is expressed.

>95% as determined by SDS-PAGE.

b0344; JW0335

beta-D-galactosidase(lacZ)

β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. β-galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose.

Supplied as a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

<1.0 EU per μg protein as determined by the LAL method.

Store at <-20°C, minimize freeze-thaw cycles.