CW49 多肽

H-APFRMGICTTN-OH

H-Ala-Pro-Phe-Arg-Met-Gly-Ile-Cys-Thr-Thr-Asn-OH

11

95.44

C₅₁H₈₃N₁₅O₁₅S₂

1210.45

固相合成

CW49 is a small peptide composed of 11 amino acid residues with the sequence APFRMGICTTN, identified from the skin of the frog Odorrana grahami. This peptide exhibits notable pro-angiogenic activity, as demonstrated by its ability to induce tube formation in human umbilical vein endothelial cells in vitro, an effect comparable to that of vascular endothelial growth factor. In a murine full-thickness dermal wound model, CW49 enhances wound healing by promoting the formation of granulation tissue and modulating epidermal thickness. Mechanistically, it upregulates the expression of key angiogenic proteins, including hypoxia-inducible factor-1 alpha, endothelial nitric oxide synthase, and inducible nitric oxide synthase, without significantly altering vascular endothelial growth factor levels. In addition to its angiogenic properties, CW49 displays anti-inflammatory activity by reducing the infiltration of macrophages and decreasing the levels of pro-inflammatory cytokines such as interleukin-6 and tumor necrosis factor-alpha in wound tissue. In vitro experiments with lipopolysaccharide-stimulated macrophages confirm that CW49 inhibits the secretion of these cytokines while promoting inducible nitric oxide synthase expression and nitric oxide production. This peptide does not significantly affect re-epithelialization or wound closure rates, suggesting its primary roles involve angiogenesis and modulation of the inflammatory response. CW49 represents a promising candidate for further investigation as a bioactive molecule with potential applications in tissue repair and regenerative biology.

一般情况下， CW49 多肽 粉末应该保存在-20甚至-70摄氏度. CW49 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Liu H, Duan Z, Tang J, Lv Q, Rong M, Lai R. A short peptide from frog skin accelerates diabetic wound healing. FEBS J. 2014 Oct;281(20):4633-43. doi: 10.1111/febs.12968. Epub 2014 Sep 8. Erratum in: FEBS J. 2026 Jan;293(1):318-319. doi: 10.1111/febs.70331. PMID: 25117795.

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。