CF-14 多肽

H-RIVELTLPRVSVRL-NH2

H-Arg-Ile-Val-Glu-Leu-Thr-Leu-Pro-Arg-Val-Ser-Val-Arg-Leu-NH2

14

95.2

C₇₄H₁₃₆N₂₄O₁₈

1650.01

固相合成

CF-14(RIVELTLPRVSVRL-NH2) is a novel antibacterial peptide, derived from the epidermal mucus of catfish. CF-14 showed antibacterial activity against S. putrefaciens and S. aureus, as well as Escherichia coli compared to negative controls. When the concentration of CF-14 was 62.5 μg/mL, the antibacterial effect of CF-14 against S. putrefaciens was similar to that of 50 μg/mL of ampicillin. CF-14 displays potent activity against gram-negative (S. putrefaciens) bacteria and gram-positive bacteria (S. aureus). Further, the MIC of CF-14 against S. putrefaciens was 62.5 μg/mL. The antimicrobial activity of CF-14 showed no obvious change after heat treatment for 30 min at either at 40 °C, 60 °C, or 80 °C, and slight inactivation after treatment at 100 °C for 30 min. CF-14 remained stable after 24 h after exposure to a pH values ranging from 4.0 to 12.0, but the antimicrobial activity of CF-14 decreased after exposure to an acidic environment (pH < 4). Finally, CF-14 was susceptible to proteases as no antimicrobial activity was observed after treatment with trypsin, pepsin, or proteinase K. CF-14 has the ability to enhance the membrane permeability of S. putrefaciens. Moreover, observations performed using scanning electron and confocal microscopy indicated that CF-14 could penetrate into the cell membranes of S. putrefaciens and accumulate in bacterial cells, but did not break down cell membranes. Further, electrophoresis analysis demonstrated that CF-14 possesses DNA-binding affinity.

一般情况下， CF-14 多肽 粉末应该保存在-20甚至-70摄氏度. CF-14 多肽 溶解后应该-70摄氏度分装保存，避免反复冻融. 了解更多细节，请查阅手册:多肽溶解及保存指南

• Li T, Liu Q, Wang D, Li J. Characterization and antimicrobial mechanism of CF-14, a new antimicrobial peptide from the epidermal mucus of catfish. Fish Shellfish Immunol. 2019

三氟乙酸（Trifluoroacetic acid，简称 TFA）是高效液相色谱（简称 HPLC）纯化过程中常用的反离子。TFA 的存在可能会对肽的净重量、外观及溶解度产生影响，具体如下：

对净重量的影响：TFA 盐会增加产物的总质量。在大多数情况下，肽的含量占总重量的 80% 以上，剩余部分则为 TFA。

对溶解度的影响：TFA 盐通常能提高肽在水溶液中的溶解度。

在生物检测中的影响：对于大多数标准的体外检测，残留的 TFA 水平一般不会造成干扰。但需注意，在高灵敏度的细胞研究或生化研究中，需关注其存在可能带来的影响。

干品多肽的重量中不仅仅包含多肽，还包含有一些非肽的组份，如水、被吸收的溶剂、配位离子和盐等。肽的净含量是指肽在其中的重量百分比，这个百分比的数值范围很大，可能从50%到90%，取决于纯度、序列以及合成和纯化的方法，不要将肽的净含量和肽的纯度混为一谈，他们是两个完全不同的概念。纯度通常是通过HPLC测定的。纯度定义的是多肽样品中含正确序列的组分的百分比，而肽的净含量是指样品中肽类物质相对于总物质所占的百分比，肽的净含量通常是氨基酸组分分析或紫外分光法测定的，这个信息主要是在一些对肽的浓度很敏感的实验中，对计算肽的浓度是很重要的。