- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, WB, IHC-P, IP
- 特异性:Human DCX
产品详情
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产品名称
Anti-DCX antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to DCX
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抗体应用
ELISA, WB, IHC-P, IP
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应用推荐
WB: 10-20 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DCX. The detection limit for Human DCX is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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特异性
Human DCX
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蛋白别名
Dbct, DC, DBCN, LISX, SCLH, XLIS, DBCN, Dbct, DC, doublecortex, doublecortex, doubling, doubling, lissencephalin-X, lissencephalin-X, lissencephaly, LISX, lis-X, lis-X, neuronal migration protein doublecortin, neuronal migration protein doublecortin, SCLH, X-linked (doublecortin), XLIS
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制备方法
Produced in rabbits immunized with purified, recombinant Human DCX (rh DCX; O43602-2; Ala45-Val150). DCX specific IgG was purified by Human DCX affinity chromatography.
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组分
0.2 μm filtered solution in PBS with 5% trehalose
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
DCX (doublecortin, N-GST chimera)contains 2 doublecortin domains and belongs to the doublecortin family. It is highly expressed in neuronal cells of fetal brain, but not expressed in other fetal tissues. In the adult, it is highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas. DCX is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. DCX may in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. It may be part with LIS-1 of a overlapping, but distinct, signaling pathways that promote neuronal migration. Defects in DCX are the cause of lissencephaly X-linked type 1 and subcortical band heterotopia X-linked.
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参考文献
- Des Portes V, et al. (1998) A novel CNS gene required for neuronal migration and involved in X-linked subcortical laminar heterotopia and lissencephaly syndrome. Cell. 92:51-61.
- Gleeson J G, et al. (998) Doublecortin, a brain-specific gene mutated in human X-linked lissencephaly and double cortex syndrome, encodes a putative signaling protein. Cell. 92:63-72.
- Ross M T, et al. (2005) The DNA sequence of the human X chromosome. Nature. 434:325-37.