- 抗体类型:多克隆
- 抗体来源:兔
- 抗体应用:ELISA, IHC-P
- 特异性:Human CREB1
产品详情
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产品名称
Anti-CREB1 antibody
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抗体类型
多克隆
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抗体来源
兔
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抗体亚型
兔IgG
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抗体描述
Rabbit polyclonal to CREB1
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抗体应用
ELISA, IHC-P
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应用推荐
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CREB1. The detection limit for Human CREB1 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
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特异性
Human CREB1
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蛋白别名
CREB, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, 2310001E10Rik, 3526402H21Rik, AV083133, Creb, CREB, Creb-1, MGC9284
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制备方法
Produced in rabbits immunized with purified, recombinant Human CREB1 (rh CREB1; P16220-2; Met1-Asp327). CREB1 specific IgG was purified by Human CREB1 affinity chromatography.
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组分
0.2 μm filtered solution in PBS with 5% trehalose
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储存方法
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
背景介绍
CAMP responsive element binding protein 1, also known as CREB-1, plays multiple functions as a transcription factor in gene regulation. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. CREB1 proteins are also known to be expressed in several spliced isoforms that act as transcriptional activators or repressors. The activator isoforms, possessing the functional domains for kinase induction and for interaction with other transcriptional regulators, act as transcriptional activators.The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB-1 has a complex influence on behavioural responses to drugs of abuse which varies depending on the brain region in which it is expressed. CREB-1 is important for serotonin (5-HT)-induced long-term facilitation (LTF) of the sensorimotor synapse in Aplysia.
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参考文献
- Sadamoto H, et al. (2011) Direct observation of dimerization between different CREB1 isoforms in a living cell. PLoS One. 6 (6): e20285.
- Liu RY, et al. (2011) The requirement for enhanced CREB1 expression in consolidation of long-term synaptic facilitation and long-term excitability in sensory neurons of Aplysia. J Neurosci. 31 (18): 6871-9.
- Hoeffler JP, et al. (1988) Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA. Science. 242 (4884): 1430-3.