重组人ACOT13蛋白

人

大肠杆菌

Met 1 - Asn 140

A DNA sequence encoding the human THEM2 (NP_060943.1) (Met1-Asn140) was expressed with a polyhistidine tag at the N-terminus.

> 85 % as determined by SDS-PAGE

The recombinant human THEM2 consists of 158 amino acids and predicts a molecular mass of 17.2 KDa. It migrates as an approximately 18 KDa band in SDS-PAGE under reducing conditions.

Acyl-CoA thioesterase 13

Acyl-coenzyme A thioesterase 13

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA and 3,4-dihydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis (By similarity).

Lyophilized from sterile 50 mM Tris, 100 mM Arg, pH 8.0.
1. 5 % trehalose and mannitol are added as protectants before lyophilization.
2. Please contact us for any concerns or special requirements.

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Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.