常用测序通用引物大全

下面是我们从网上搜集的最全的常用测序通用引物列表:

测序通用引物列表

引物名称序列(5'→3')描述
3'AOX1GCAAATGGCATTCTGACATCCFor Pichia vectors with AOX1 terminator, reverse primer
5'AOX1GACTGGTTCCAATTGACAAGCFor Pichia vectors with AOX1 promoter, forward primer
Polyhedrin forwardAAATGATAACCATCTCGCPolyhedrin promoter, forward primer
pGP704-RAACAAGCCAGGGATGTAACGR6K gamma origin, 3' of MCS in pGP704 vector, reverse primer
SeqL-B (ATTL2)AACATCAGAGATTTTGAGACAC
BAC1AACCATCTCGCAAATAAATA
PIRES3-EGFP-RAACGCACACCGGCCTTATTC
Ubx-FAACTCGTACTTTGAACAGGCDrosophila Ultrabithorax gene, forward primer
PBV220FAAGAAGGGCAGCATTCAAAG
pbd-gal4-rAAGAGTTACTCAAGAACAAGAA
pMRB101-FAAGATGCAGGCAGCTGAGTTHCMV major immediate-early protein (IE), forward primer
PB42AD-RAAGCCGACAACCTTGATTGGAG
T7EEVAAGGCTAGAGTACTTAATACG
Gal4-ADAATACCACTACAATGGAT3' end of Gal4 activation domain, forward primer
GAL4-ADforAATACCACTACAATGGATGATG3' end of Gal4 activation domain, forward primer
pBRforEcoAATAGGCGTATCACGAGGCIn pBR322, upsteam of EcoRI site, forward primer
GAL1AATATACCTCTATACTTTAACGTCS. cerevisiae GAL1 promoter, forward primer
NOSRAATCATCGCAAGACCGGC
GW-5'AATCTCGCCGGATCCTAACT5' end of Gateway cassette, reverse primer
AC5ACACAAAGCCGCTCCATCAGDrosophila Actin 5C promoter, forward primer
T7(17base)ACATCCACTTTGCCTTTCTC
RCAS-FACATGGGTGGTGGTATAGCGCTTGCG3' of Rous sarcoma virus (RSV) env gene, forward primer
GST-TagACCCAATGTGCCTGGATGCG
pBABE 3'ACCCTAACTGACACACATTCCSV40 enhancer, 3' of MCS in pBABE vectors, reverse primer
V5 ReverseACCGAGGAGAGGGTTAGGGATV5 epitope, reverse primer
pLNCX-RACCTACAGGTGGGGTCTTTCATTCCC
BAC2ACGCACAGAATCTAGCGCTT
pLTet-FACTGAGCACATCAGCAGGACLambda phage early leftward (pL) promoter, forward primer
pCEP-FAGAGCTCGTTTAGTGAACCG
F27AGAGTTTGATCCTGGCTCA
3'ADAGATGGTGCACGATGCACAG
L4440AGCGAGTCAGTGAGCGAG5' of MCS in L4440 vector, forward primer
M13 Reverse  (-48)AGCGGATAACAATTTCACAC
M13/pUC ReverseAGCGGATAACAATTTCACACAGGIn lacZ gene
M13R(-48)AGCGGATAACAATTTCACACAGGAIn lacZ gene
LNCXAGCTCGTTTAGTGAACCGTCAGATCHuman CMV promoter, forward primer
pLNCX-FAGCTCGTTTAGTGAACCGTCAGATCG
DsRed1-CAGCTGGACATCACCTCCCACAACG3' end of DsRed1, forward primer
2.0rev primerAGGCGATTAAGTTGGGTA
CMV-ReverseAGTAGGAAAGTCCCGTAAGG
Luc-FAGTCAAGTAACAACCGCGA3' end of luciferase, forward primer
N-CMV-30ATAACCCCGCCCCGTTG
Amp-RATAATACCGCGCCACATAGC5' end of ampicillin resistance gene, reverse primer
MMLV-FATCAGTTCGCTTCTCGCTTCMoloney murine leukemia virus LTR (MoMuLV), forward primer
pBAD ForwardATGCCATAGCATTTTTATCCFor vectors with E. coli araBAD promoter, forward primer
pET-UpstreamATGCGTCCGGCGTAGA
U6ATGGACTATCATATGCTTACCGTA
pENTR-RATGGCTCATAACACCCCTTG3' of attL2 in pENTR vector, reverse primer
T3ATTAACCCTCACTAAAGGGAT3 promoter, forward primer
pCMV5RATTATAGAGGACACCTAGTC
pDsRed-C-3'ATTCATTTTATGTTTCAGGTTCA
SFFV-FATTGATTGACTGCCCACCTCSpleen focus forming virus 5' LTR, forward primer
SP6ATTTAGGTGACACTATAGSP6 promoter, forward primer
S1CAACGTGAAAAAATTATTATTCGC
TRC-FCAAGGCTGTTAGAGAGATAATTGGAHuman U6 promoter, forward primer
T3CAATTAACCCTCACTAAAGG
AUG1 ForwardCAATTTACATCTTTATTTATTAACGFor Pichia vectors with AUG1 promoter, forward primer
SV40-spliceRCACAAAGATCCGGACCAAAGSV40 splice sequence, reverse primer
pIRES.RCACTGCATTCTAGTTGTGGT
mU6-FCAGCACAAAAGGAAACTCACCMouse U6 promoter, forward primer
MSCV-revCAGCGGGGCTGCTAAAGCGCATGCMurine stem cell virus, reverse primer
M13 ReverseCAGGAAACAGCTATGACIn lacZ gene
M13RCAGGAAACAGCTATGACC
mU6CAGTGCAGGGGAAAGAATAGTAGAC
WPRE-RCATAGCGTAAAAGGAGCAACA5' end of WPRE, reverse primer
MT ForwardCATCTCAGTGCAACTAAADrosophila metallothionein promoter, forward primer
EGFP-CCATGGTCCTGCTGGAGTTCGTG3' end of EGFP, forward primer
mPGK-FCATTCTGCACGCTTCAAAAGMouse PGK promoter, forward primer
pTRE 3'CCACACCTCCCCCTGAAC
PB42AD-FCCAGCCTCTTGCTGAGTGGAGATG
hGH-PA-RCCAGCTTGGTTCCCAATAGAHuman growth hormone terminator, reverse primer
Rluc-FCCAGGATTCTTTTCCAATGC3' end of Renilla luciferase, forward primer
pTarget.FCCAGGATTTTCCCAGTCAC
pCAMBIA1301-5'CCAGGCTTTACACTTTATGC
GFP-RCCATCTAATTCAACAAGAATTGGGACAAC5' end of GFP, reverse primer
M13/pUC ForwardCCCAGTCACGACGTTGTAAAACGIn lacZ gene
mCherry-FCCCCGTAATGCAGAAGAAGA3' end of mCherry, forward primer
pQE promoterCCCGAAAAGTGCCACCTG5' of MCS in pQE vectors, forward primer
Intein ForwardCCCGCCGCTGCTTTTGCACGTGAG
M13(-96)CCCTCATAGTTAGCGTAACG
MSCV|pLXSN 5'CCCTTGAACCTCCTCGTTCGACCMurine stem cell virus, forward primer
pGEX 3'CCGGGAGCTGCATGTGTCAGAGG3' of MCS in pGEX vectors, reverse primer
IRES-RCCTCACATTGCCAAAAGACG5' end of IRES, reverse primer
SV40-pArevCCTCTACAAATGTGGTATGG
LucNrevCCTTATGCAGTTGCTCTCC5' end of luciferase, reverse primer
S.tagCGAACGCCAGCACATGGACA
CMV ForwardCGCAAATGGGCGGTAGGCGTGHuman CMV immediate early promoter, forward primer
OpIE2 ForwardCGCAACGATCTGGTAAACACOpIE2 promoter, forward primer
M13F(-47)CGCCAGGGTTTTCCCAGTCACGAC
pTRE 5'CGCCTGGAGACGCCATCC
pBluescript SKCGCTCTAGAACTAGTGGATC
pRS-markerCGGCATCAGAGCAGATTGTATo sequence yeast selectable marker in pRS vectors
GPDpro-FCGGTAGGTATTGATTGTAATTCTGS. cerevisiae GPD promoter, forward primer
pLEXA-FCGTCAGCAGAGCTTCACCAT
LexACGTCAGCAGAGCTTCACCATTG3' end of LexA DNA binding domain, forward primer
EGFP-NrevCGTCGCCGTCCAGCTC
EGFP-NCGTCGCCGTCCAGCTCGACCA
EGFP-NCGTCGCCGTCCAGCTCGACCAG5' end of EGFP, reverse primer
PinPoint  primerCGTGACGCGGTGCAGGGCG
Neo-FCGTTGGCTACCCGTGATATT3' end of neomycin resistance gene, forward primer
pENTR-FCTACAAACTCTTCCTGTTAGTTAG5' of attL1 in pENTR vector, forward primer
PCDNA3.1FCTAGAGAACCCACTGCTTAC
RVprimer3CTAGCAAAATAGGCTGTCCC5' of MCS in pGL3 vector, forward primer
35S promoterCTATCCTTCGCAAGACCCTTCCaMV 35S promoter, forward primer
5'TriplEx2CTCCGAGATCTGGACGAGC
Ecdysone ForwardCTCTGAATACTTTCAACAAGTTACDrosophila heat shock promoter, forward primer
pFastBac-3'CTGATTATGATCCTCTAGTACTTC
PBV220RCTGCGTTCTGATTTAATCTG
Bglob-intron-FCTGGTCATCATCCTGCCTTTRabbit beta-globin intron, forward primer
tdTomato-FwdCTGTTCCTGTACGGCATGG3' end of tdTomato, forward primer
pIRES-FCTTACTGACATCCACTTTGC
Pry1CTTAGCATGTCCGTGGGGTTTGAATPZ P-element, reverse primer
TKpolyAreverseCTTCCGTGTTTCAGTTAGC
pLXSN-FCTTGAACCTCCTCGTTCGAC
pBRforBamCTTGGAGCCACTATCGACIn pBR322 tet region, upstream of BamHI, forward primer
pBABE 5'CTTTATCCAGCCCTCACPsi packaging signal, 5' of MCS in pBABE vectors, forward  primer
GLP2CTTTATGTTTTTGGCGTCTTCCA
SV40pA-RGAAATTTGTGATGCTATTGCSV40 polyA, reverse primer
S.tagGAACGCCAGCACATGGAC
AUG1 ReverseGAAGAGAAAAACATTAGTTGGCFor Pichia vectors with AUG1 promoter, reverse primer
LacZ-RGACAGTATCGGCCTCAGGAA5' end of LacZ, reverse primer
RVP4GACGATAGTCATGCCCCGCG
pbi-121(35s)GACGCACAATCCCACTATCC
pMX-S1811GACGGCATCGCAGCTTGGATACACMMLV sequence, 5' of MCS in pMXs vector, forward primer
HU6GACTATCATATGCTTACCGT
XBG-RGACTCCATTCGGGTGTTCXenopus beta-globin 3'UTR, reverse primer
Tac promoterGAGCGGATAACAATTTCACACAGGTac promoter, forward primer
HATGAGGAGCACGCTCATGCCCACHistidine affinity tag, forward primer
pTrcHis ForwardGAGGTATATATTAATGTATCG5' of MCS in pTrcHis vector, forward primer
Gal4 N-termGAGTAGTAACAAAGGTCAA3' end of Gal4 DNA binding domain, forward primer
pHybLex ReverseGAGTCACTTTAAAATTTGTATACACADH terminator, reverse primer
3.0revGAGTTAGCTCACTCATTAGGC
NOSGATAATCATCGCAAGACCGG
pCMVFGATCCGGTACTAGAGGAACTGAAAAAC
DuetUP1GATCTCGACGCTCTCCCT
MBP-FGATGAAGCCCTGAAAGACGCGCAG3' end of maltose binding protein, forward primer
pYESTrp  ForwardGATGTTAACGATACCAGCC
DuetDOWN1GATTATGCGGCCGTGTACAA
pBAD Reverse|pTrcHis ReverseGATTTAATCTGTATCAGGFor vectors with E. coli araBAD promoter, reverse primer
CRE-RGCAAACGGACAGAAGCATTT5' end of Cre recombinase, reverse primer
Puro-FGCAACCTCCCTTCTACGAGC3' end of puromycin resistance gene, forward primer
pCAG-FGCAACGTGCTGGTTATTGTGRabbit beta-globin intron, for pCAG plasmids, forward primer
pCasper-hsGCAACTACTGAAATCTGCCAAGDrosophila Hsp70 promoter, forward primer
CAT-RGCAACTGACTGAAATGCCTC5' end of chloramphenicol resistance gene, reverse primer
Nmt1-FGCAATGTGCAGCGAAACTAAS. pombe nmt1 promoter, forward primer
T3GCAATTAACCCTCACTAAAGGT3 promoter, forward primer
pFlag-CMV-RGCACTGGAGTGGCAACTT
MycGCATCAATGCAGAAGCTGATCTCAMyc tag, forward primer
GW-3'GCATGATGACCACCGATATG3' end of Gateway cassette, forward primer
Neo-RGCCCAGTCATAGCCGAATAG5' end of neomycin resistance gene, reverse primer
SV40-PromoterGCCCCTAACTCCGCCCATCC
pbd-gal4-fGCCTCTAACATTGAGACAGC
SeqL-A (ATTL1)GCGAGAGTAGGGAACTGC
pCAMBIA1301-3'GCGATTAAGTTGGGTAACGC
CYC1|pYESTrp ReverseGCGTGAATGTAAGCGTGACCYC1 transcription termination signal, reverse primer
NOS-FGCGTTCAAAAGTCGCCTAAGNopaline synthase promoter, forward primer
pAd-CMVGCTAGAGATCTGGTACCGTCFor cloning sites after SalI in pAd-CMV vector
T7 TerminalGCTAGTTATTGCTCAGCGGT7 terminator, reverse primer
pREP ForwardGCTCGATACAATAAACGCCRous sarcoma virus (RSV) promoter, forward primer
MT1-FGCTGTCCTCTAAGCGTCACCMouse metallothionein 1 promoter, forward primer
pBMN 5'GCTTGGATACACGCCGCMMLV sequence, for inserts in pBMN retroviral vector
M13 Reverse  (-27)GGAAACAGCTATGACCATG
SV40-FGGAGGCTTTTTTGGAGGC
3'AOXGGCAAATGGCATTCTGACAT
PCDNA3.0-R2GGCAACTAGAAGGCACAGTC
LacI-RGGCATACTCTGCGACATCGT5' end of LacI, reverse primer
Tet-RGGCGAGTTTACGGGTTGTTA5' end of tetracycline resistance gene, reverse primer
pTarget.RGGCTTTACACTTTATGCTTC
pBR322ori-FGGGAAACGCCTGGTATCTTTpBRS322 origin, forward primer
U6 PrimerGGGCAGGAAGAGGGCCTAT
pGEX 5'GGGCTGGCAAGCCACGTTTGGTG3' end of glutathione-S-transferase, forward primer
Tn7-endGGGGTGGAAATGGAGTTTTTBacterial transposon Tn7
pCasper-FGGGTTTTATTAACTTACAT5' end of Drosophila mini-white gene, reverse primer
pFlag-CMV-FGGTAGGCGTGTACGGTGG
GFP-FGGTCCTTCTTGAGTTTGTAAC3' end of GFP, forward primer
MalE PrimerGGTCGTCAGACTGTCGATGAAGCC
pSHUTTLE-CMV-FGGTCTATATAAGCAGAGGTG
pBRrevBamGGTGATGTCGGCGATATAGGIn pBR322 tet region, downstream of BamHI, reverse primer
Gal10pro-FGGTGGTAATGCCATGTAATATGS. cerevisiae GAL10 promoter, forward primer
1492RGGTTACCTTGTTACGACTT
M13 Forward  (-20)GTAAAACGACGGCCAGTG
S6GTAAATGAATTTTCTGTAGTAGG
pPc86-3'GTAAATTTCTGGCAAGGTAGAC
DsRed1-NGTACTGGAACTGGGGGGACAG5' end of DsRed1, reverse primer
PIRES2-EGFP-FGTAGGCGTGTACGGTGGGAG
Polyhedrin reverseGTCCAAGTTTCCCTGFor baculovirus vector with polyhedrin promoter, reverse  primer
pFastBac-5'GTCCGAAACCATGTCGTAC
EXFP-RGTCTTGTAGTTGCCGTCGTCFor distinguishing EGFP vs ECFP vs EYFP, reverse primer
pACT2-RGTGCACGATGCACAGTTGAA
F1ori-FGTGGACTCTTGTTCCAAACTGGF1 origin, forward primer
pSHUTTLE-CMV-RGTGGTATGGCTGATTATGATCAG
EBV ReverseGTGGTTTGTCCAAACTCATCSV40 polyA terminator, reverse primer
CMV-RGTTCACGGTGCCCTCC
pQE30-RGTTCTGAGGTCATTACTGG
pcDL-FGTTGCCTTTACTTCTAGGCCT5' of EcoRI site in pcDL vector, forward primer
pLXSN-RGTTGCTGACTAATTGAGATG
M13 (-40)GTTTTCCCAGTCACGACIn lacZ gene
pLEXA-RTAAAACCTAAGAGTCACTTT
3'BDTAAGAGTCACTTTAAAATTTGTA
T7TAATACGACTCACTATAGGGT7 promoter, forward primer
GAL4 ADTACCACTACAATGGATG
HA-FTACCCATACGACGTCCCAGAHA tag, forward primer
SP6TACGATTTAGGTGACACTATAG
α-FactorTACTATTGCCAGCATTGCTGC
BGH Reverse|pcDNA3.1RTAGAAGGCACAGTCGAGGBovine growth hormone terminator, reverse primer
pPc86FTATAACGCGTTTGGAATCACT
Xpress ForwardTATGGCTAGCATGACTGGTXpress epitope, forward primer
PEGFP-CTATGGCTGATTATGATCAGT
SV40pro-FTATTTATGCAGAGGCCGAGGSV40 promoter/origin, forward primer
EF-1a ForwardTCAAGCCTCAGACAGTGGTTCHuman elongation factor-1a promoter, forward primer
GAL4 BDTCATCGGAAGAGAGTAG
GAL4-BDforTCATCGGAAGAGAGTAGTAA
pCMV-RTCCAAACTCATCAATGTATC
pDsRED-express-C1-FTCCCACAACGAGGACTACAC
hrGFP-RTCCCCGAGTACCACTTCATChrGFP (humanized Renilla GFP), forward primer
ITS1TCCGTAGGTGAACCTGCGG
ITS4TCCTCCGCTTATTGATATGC
pZIPTCCTTTCCAGCGAGGTTCTAMurine leukemia virus (MuLV), reverse primer
pBluescriptKSTCGAGGTCGACGGTATCFor pBluescript vector
H1TCGCTATGTGTTCTGGGAAAHuman H1 promoter, forward primer
pYes2.RTCGGTTAGAGCGGATGTG
pCMV-FTCTAAAAGCTGCGGAATTGT
pBluescriptSKTCTAGAACTAGTGGATCFor pBluescript vector
HA-RTCTGGGACGTCGTATGGGTAHA tag, reverse primer
tdTomato-RevTCTTTGATGACGGCCATGT5' end of tdTomato, reverse primer
pDSRED-N1-RTGAAGCGCATGAACTCCTTG
hUBCpro-FTGAAGCTCCGGTTTTGAACTHuman Ubiquitin C (UbC) promoter, forward primer
pACYC-FTGAAGTCAGCCCCATACGATp15A origin, forward primer
pQE30-FTGAGCGGATAACAATTTCAC
P5'|pMAL-C2X-FTGCGTACTGCGGTGATCAAC
T7TERTGCTAGTTATTGCTCAGCGG
IRES-FTGGCTCTCCTCAAGCGTATT3' end of IRES, forward primer
pEGFP-N-5’TGGGAGGTCTATATAAGCAGAG
M13 (-21) ForwardTGTAAAACGACGGCCAGTIn lacZ gene
GLP1TGTATCTTATGGTACTGTAACTG
C-CMV-24TTAGGACAAGGCTGGTGG
PCMV-5FTTCCAAAATGTCGTAATAAC
pMT2-FTTGCCTTTCTCTCCACAGGT3' end of synthetic intron, forward primer
pTRC99C-FTTGCGCCGACATCATAAC
mCherry-RTTGGTCACCTTCAGCTTGG5' end of mCherry, reverse primer
DuetUP2TTGTACACGGCCGCATAATC
TK-pA-RTTGTCTCCTTCCGTGTTTCAThymidine kinase polyA, reverse primer
XEF1aTTTCGCCCTAACTTCGTGATXenopus EF1 alpha enhancer/promoter, forward primer
Bglob-intron-RTTTGCCCCCTCCATATAACARabbit beta-globin intron, reverse primer
P12584TTTTCAGTATCTACGAT
Bglob-pA-RTTTTGGCAGAGGGAAAAAGARabbit beta-globin polyA region, reverse primer


Souce: 纽普生物    2017-03-01