LOCUS       V032659                 8981 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032659
VERSION     V032659
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..4474
                     /label="ZC3H6(NM_198581)"
                     /note="ZC3H6(NM_198581)"
                     /gene="ZC3H6"
     CDS             4481..4507
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4548..4772
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4818..5246
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5260..5589
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5656..6450
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6624..6745
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6794..6810)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6818..6834
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6842..6872)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6887..6908
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7196..7781)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7952..8812)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8813..8917)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg acagactctg aacatgcagg gcacgacaga gaagatggcg aattagaaga
      961 tggtgaaata gacgatgcag gatttgaaga aatacaagaa aaagaagcaa aagagaatga
     1021 aaagcagaaa agtgagaaag cctacagaaa atcaagaaaa aaacataaga aagagagaga
     1081 gaagaaaaaa tccaaaagga gaaaacgtga gaaacataag cataattccc catctagtga
     1141 tgatagttcg gactacagcc ttgattcaga tgttgaacat acagaaagtt cccataaaaa
     1201 aagaactggt ttctacaggg attatgacat tccatttact cagcgtggac atatatcagg
     1261 aagctacata acatcaaaga agggtcaaca taacaaaaaa tttaaaagta aagaatatga
     1321 tgagtacagc acctacagtg atgacaactt cggtaactac agtgatgaca actttggtaa
     1381 ctacggtcag gaaacagagg aagattttgc caatcagctg aaacaataca ggcaagctaa
     1441 agaaacctca aatattgctt tagggtcatc attttctaaa gaatcaggaa aaaaacagag
     1501 aatgaaagga gttcagcaag gtattgaaca gagagttaaa agttttaatg ttggtcgtgg
     1561 acgtggcttg ccgaagaaaa tcaaacgaaa agaacgtggg ggaagaacca ataaagggcc
     1621 taatgtgttt tcagtatcgg atgactttca agagtataat aaaccaggga aaaaatggaa
     1681 ggttatgact caggaattta ttaatcagca cacagtggaa cacaaaggaa aacaaatctg
     1741 taaatacttc ctggaaggga ggtgtattaa gggagatcag tgtaaatttg atcatgatgc
     1801 agagttggag aaaagaaaag agatctgcaa attttattta caaggatatt gtaccaaagg
     1861 agagaactgc atttatatgc ataatgaatt tccatgcaag ttctatcata gtggagcaaa
     1921 atgttaccag ggagacaact gtaaattttc ccatgatgat ctaactaaag aaacaaagaa
     1981 acttttggac aaagtgttga atactgatga agaactcata aatgaagatg aaagagaatt
     2041 agaggaactt agaaagcgtg gcataactcc tcttcccaaa ccacctccag gggttgggct
     2101 tctgccaacc cctccagagc attttccctt ttctgatcct gaagacgatt ttcagacaga
     2161 tttctctgat gattttagga aaattccatc tctttttgaa atagttgtaa aacctactgt
     2221 ggatttagcg cataaaattg ggaggaagcc accagcattt tataccagtg cctcaccacc
     2281 aggaccacaa tttcagggaa gcagtccaca ccctcaacat atctatagtt ctgggtcaag
     2341 tccaggtcct ggacctaaca tgtctcaggg acacagtagt cctgtgatgc acccaggctc
     2401 ccctggacat cacccatgtg caggacctcc tggtctacca gtgccacaga gcccaccttt
     2461 accacctggt ccacctgaaa ttgtaggtcc tcaaaatcaa gctggagtgc ttgttcaacc
     2521 agacacatct ttgacaccac caagtatggg tggggcttac cactccccag gctttccagg
     2581 acatgtgatg aaagtaccca gagagaatca ctgttctcca ggttcatcat accagcaaag
     2641 tcctggtgaa atgcagctca acaccaatta tgagtccctg caaaacccag ctgagtttta
     2701 cgataattac tatgcacagc attctataca taattttcag ccacccaata actctggtga
     2761 tgggatgtgg catggtgaat ttgcccagca gcagcctcct gttgttcaag actcacctaa
     2821 ccatgggagt gggtctgatg gcagcagcac taggacaggc catggccctc tgcctgtacc
     2881 aggcctcctc cctgcagtgc aaagagctct ttttgtaaga cttactcaga gataccaaga
     2941 agatgaagaa caaaccagca cccaacctca tagggcacca agcaaggaag aagatgatac
     3001 agttaactgg tattccagta gtgaagagga agaaggaagc agtgtcaaat caatactgaa
     3061 aacattacag aaacaaacag aaactttaag gaatcagcaa caaccttcca cagaactcag
     3121 cactcctact gatccaagac ttgctaaaga gaaaagtaaa ggaaaccaag tggttgaccc
     3181 taggcttagg actatcccaa ggcaagacat tagaaagcct tctgagtctg ccccactgga
     3241 tcttagactt gcgtgggatc ccaggaaatt gagagggaat ggaagtggtc acataggctc
     3301 ttctgttggt ggagcaaagt ttgatttgca tcatgcaaat gctggcacta atgtcaaaca
     3361 caaaagaggc gatgatgatg atgaagatac agaaagagaa ctgagagaaa aagctttctt
     3421 aatacctttg gatgcctcac ctggcataat gctccaggat ccaaggtcac aattgagaca
     3481 gttcagtcac attaaaatgg acattactct aaccaaaccc aactttgcaa aacacatcgt
     3541 gtgggctccc gaagacttac ttccagtacc tttacctaaa cctgatccag tgtcttcaat
     3601 caatttacct ctgcccccac ttatagctga ccagaggcta aatagattat ggaatacaaa
     3661 aagtgatctt catcaaaata cagtgtccat tgatccaaaa ttagcagcca aagccaaaat
     3721 taacacaaca aacagagaag gctacctaga acaatttgga gactcacacg gttcaggagc
     3781 taaattagga gatcctagac tacaaaaaaa ttttgatcct aggcttcaca gactgcccaa
     3841 tacagagtct catcaagtgg ttatgaagga ttcacatgca tcaaagggtg cccctcactt
     3901 acccagatca aaccctggtt catcacagcc ctcaggggca ggaactagca attctggttc
     3961 cggggctctg cctccatatg cccctaaact ctcttcctca gctggccttc cactgggaac
     4021 ttccacttca gttcttagtg gtattagttt gtatgaccct agggatcacg gttcatcatc
     4081 cacatcagag ctagcaacag cttcttcagg agaaaactca aagaaccaga aaaaaagtgg
     4141 tggcttaaaa agtagtgaca aaactgaacc ttctcctgga gaagccatcc ttccacaaaa
     4201 acccagtcca aacgtgggag tcactcttga ggggccagct gacccacagg cggacgttcc
     4261 caggagttct ggtaaggttc aggtcccagc agtgcacagc cttcctgttc aggcattaac
     4321 aggcttaatt aggccacagt acagtgatcc aaggcaggca aggcagccag gacaggggag
     4381 cccgacccca gataatgatc ccggtagaga aacagatgac aaatctctga aagaggtttt
     4441 taaaactttt gatccaactg cttcaccatt ttgtctcgag tacccatacg acgtaccaga
     4501 ttacgcttag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     4561 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     4621 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     4681 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     4741 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     4801 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4861 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4921 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4981 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     5041 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5101 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     5161 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     5221 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     5281 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5341 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5401 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5461 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5521 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5581 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     5641 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     5701 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     5761 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     5821 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     5881 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     5941 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     6001 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     6061 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     6121 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     6181 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     6241 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     6301 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     6361 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     6421 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     6481 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     6541 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     6601 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     6661 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6721 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     6781 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     6841 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     6901 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     6961 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7021 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7081 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7141 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7201 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     7261 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     7321 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     7381 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     7441 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     7501 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     7561 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     7621 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     7681 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtttttttg
     7741 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7801 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7861 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7921 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7981 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8041 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8101 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8161 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8221 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8281 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8341 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8401 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8461 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8521 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8581 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8641 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8701 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8761 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8821 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8881 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8941 ctgacgtcga cggatcggga gatctcccga tcccctatgg t
//
