LOCUS       V039835                 8919 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039835
VERSION     V039835
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..952
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             959..988
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             995..1024
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     CDS             1043..4405
                     /label="WDR6(NM_018031)"
                     /note="WDR6(NM_018031)"
                     /gene="WDR6"
     polyA_signal    4486..4710
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4756..5184
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5198..5527
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5594..6388
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6562..6683
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6732..6748)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6756..6772
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6780..6810)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6825..6846
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7134..7719)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7890..8750)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8751..8855)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 ctctagaatg agcggccgct ctgagcaaaa gctcatttct gaagaggact tgaatggaga
      961 acagaaattg atcagtgagg aagacctcaa cggtgagcag aagttaatat ccgaggagga
     1021 tcttaatagt agcggccgcg gtatggacgc tctcgaggac tacgtttggc cgcgggcaac
     1081 ctcggagctt atactcctcc cagtgacggg tctggagtgc gtgggggacc ggctgttggc
     1141 gggtgagggt cccgatgtcc tggtgtacag cttggacttt ggtgggcatc tgcggatgat
     1201 aaagcgagtg cagaacctgc ttggccacta tcttatccat ggcttccggg tacggccaga
     1261 gcctaatgga gaccttgact tggaggccat ggtggctgtg tttggaagca agggactccg
     1321 agttgtgaaa attagctggg gacagggcca cttctgggag ctttggcgct ctggcctgtg
     1381 gaacatgtct gactggattt gggatgcacg ctggcttgag ggaaatatag ccttggccct
     1441 gggccacaac tcagtggtgc tatatgaccc tgtagtaggg tgcatcctgc aagaggtgcc
     1501 ctgcacagac aggtgcaccc tctcttcagc ctgcctgatt ggagacgcct ggaaggagct
     1561 gaccatagtg gcaggtgctg tttccaacca gctcttggtc tggtacccag caactgcctt
     1621 agcagacaac aaacctgtag cacctgaccg acgaatcagt gggcatgtgg gcatcatctt
     1681 cagcatgtca tacctggaaa gcaagggatt gctggctaca gcttcagaag accgaagcgt
     1741 tcgtatctgg aaggtgggcg acctgcgagt gcctgggggt cgggtgcaga atattgggca
     1801 ctgctttggg cacagcgccc gtgtgtggca ggtcaagctt ctagagaatt accttatcag
     1861 tgcaggagag gattgtgtct gcttggtgtg gagccatgaa ggtgagatcc tccaggcctt
     1921 tcggggacac cagggacgtg ggatccgggc catagctgcc catgagaggc aggcctgggt
     1981 gatcactggg ggtgatgact caggcattcg gctgtggcac ttggtagggc gtgggtaccg
     2041 gggattgggg gtctcggctc tctgcttcaa gtcccgtagt aggccaggta cactcaaggc
     2101 tgtgactctg gctggctctt ggcgactgct ggcagtgact gatacagggg ccctgtatct
     2161 ctatgacgtc gaggtcaagt gctgggagca gctgctagag gataaacatt tccagtccta
     2221 ctgcctgctg gaggcagctc ctggtcccga gggcttcgga ttgtgtgcta tggccaatgg
     2281 ggaaggtcgt gtcaaggttg tccccatcaa cactccaact gctgctgtgg accagaccct
     2341 gtttcctggg aaggtgcaca gcttgagctg ggccctgcgt ggttatgagg agctcctgtt
     2401 gctggcatcg ggccctggcg gggtagtagc ttgcctagag atctcagccg caccctctgg
     2461 caaggccatc tttgtcaagg aacgttgtcg gtacctgctg cccccaagca agcagagatg
     2521 gcacacatgc agtgccttcc tacccccagg tgacttcctg gtgtgtggtg accgccgggg
     2581 ctctgtgctg ctattcccct ccagaccagg tctgctcaag gaccctgggg tgggaggcaa
     2641 ggctcgggct ggtgctgggg cacctgtagt gggtagtggt agtagtgggg gtgggaatgc
     2701 tttcactggg ttgggcccag tgtctaccct gccctctctg cacgggaagc agggtgtgac
     2761 ctcagtcaca tgccatggtg gctatgtgta taccacaggg cgtgatggag cctactacca
     2821 gctgtttgta cgagacggcc agctccagcc agtcctaagg cagaagtcct gtcgaggcat
     2881 gaactggcta gctgggctcc gtatagtgcc cgatgggagc atggttatcc tgggtttcca
     2941 tgccaatgag tttgtggtgt ggaaccctcg gtcacacgag aagctgcaca tcgtcaactg
     3001 tggtggaggg caccgttcgt gggcattctc tgatactgag gcggccatgg cctttgctta
     3061 cctcaaggat ggggatgtca tgctgtacag ggctctgggt ggctgcaccc ggccacacgt
     3121 gattctccgg gagggtctgc atggccgtga gatcacttgt gtaaagcgtg tgggcaccat
     3181 taccctgggg cctgaatatg gagtgcccag cttcatgcag cctgatgacc tggagcctgg
     3241 cagtgagggg cccgacttga ctgacattgt gatcacatgt agtgaggaca ctactgtctg
     3301 tgtcctagca ctccctacaa ccacaggctc agcccacgca ctcacagctg tttgtaacca
     3361 tatctcctcg gtacgtgctg tggctgtgtg gggcattggc accccaggtg gccctcagga
     3421 tcctcagcca ggcctgactg cccatgtggt gtctgcgggg gggcgggctg agatgcactg
     3481 cttcagcatc atggttactc cggaccccag caccccaagc cgcctcgcct gccatgtcat
     3541 gcacctttcg tcccaccggc tagatgagta ttgggaccgg caacgcaatc ggcatcggat
     3601 ggttaaggta gacccagaga ccaggtacat gtcccttgct gtgtgtgaac ttgaccagcc
     3661 cggccttggc ccccttgtgg ctgcagcctg tagtgatggg gccgtaaggc tctttctttt
     3721 gcaggattct gggcggattc tgcagctcct tgctgaaacc ttccaccata agcgatgtgt
     3781 cctcaaggtc cactccttta cacacgaggc acccaaccag aggcggaggc tcctcctgtg
     3841 cagcgcagct actgatggca gcctggcttt ctgggatctc accaccatgc tagaccatga
     3901 ctccactgtc ctggagcctc cagtggatcc tgggcttccc taccggcttg gcaccccctc
     3961 cctgactctc caggcccaca gctgtggtat caacagcctg cacaccttgc ccacccgtga
     4021 gggccaccat ctcgtggcca gtggcagtga agatggatcc ctccatgtct tcgtgcttgc
     4081 tgtggagatg ctacagctag aagaggctgt gggagaggct gggctggtac cccagctgcg
     4141 tgtgctagag gaatactctg tcccctgtgc acatgctgcc catgtgacag gcctcaagat
     4201 cctaagccca agcatcatgg tctcagcctc cattgatcaa cggctgacct tctggcgtct
     4261 ggggcatggt gaacccacct tcatgaatag cactgtgttc catgtgcctg atgtggctga
     4321 catggactgc tggcctgtga gccctgagtt tggccaccgt tgtgcccttg ggggtcaggg
     4381 gcttgaggtt tacaactggt atgactgaga attcctgcag atatccagca cagtggcggc
     4441 cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4501 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     4561 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     4621 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     4681 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     4741 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     4801 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     4861 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     4921 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     4981 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5041 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5101 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5161 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5221 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5281 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5341 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5401 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5461 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     5521 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     5581 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5641 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5701 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5761 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5821 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5881 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     5941 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6001 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6061 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6121 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6181 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6241 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6301 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6361 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6421 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6481 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6541 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     6601 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6661 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     6721 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     6781 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     6841 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     6901 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     6961 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     7021 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     7081 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     7141 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     7201 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     7261 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     7321 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     7381 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     7441 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     7501 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     7561 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     7621 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt
     7681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7801 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7861 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7921 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     7981 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8041 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8101 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8161 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8221 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8281 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8341 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8401 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8461 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8521 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8581 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8641 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8701 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8761 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8821 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8881 gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
