LOCUS V021491 9356 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V021491 VERSION V021491 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 941..4888 /label="USP42(NM_032172)" /note="USP42(NM_032172)" /gene="USP42" CDS 4895..4918 /codon_start=1 /product="FLAG® epitope tag, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDDDDK" /label="FLAG" polyA_signal 4956..5180 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 5226..5654 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 5668..5997 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 6064..6858 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 7032..7153 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(7202..7218) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 7226..7242 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(7250..7280) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 7295..7316 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(7604..8189) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(8360..9220) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(9221..9325) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccgccacc atgaccatag ttgacaaagc 961 ttctgaatct tcagacccat cagcctatca gaatcagcct ggcagctccg aggcagtctc 1021 acctggagac atggatgcag gttctgccag ctggggtgct gtgtcttcat tgaatgatgt 1081 gtcaaatcac acactttctt taggaccagt acctggtgct gtagtttatt cgagttcatc 1141 tgtacctgat aaatcaaaac catcaccaca aaaggatcaa gccctaggtg atggcatcgc 1201 tcctccacag aaagttcttt tcccatctga gaagatttgt cttaagtggc aacaaactca 1261 tagagttgga gctgggctcc agaatttggg caatacctgt tttgccaatg cagcactgca 1321 gtgtttaacc tacacaccac ctcttgccaa ttacatgcta tcacatgaac actccaaaac 1381 atgtcatgca gaaggctttt gtatgatgtg tacaatgcaa gcacatatta cccaggcact 1441 cagtaatcct ggggacgtta ttaaaccaat gtttgtcatc aatgagatgc ggcgtatagc 1501 taggcacttc cgttttggaa accaagaaga tgcccatgaa ttccttcaat acactgttga 1561 tgctatgcag aaagcatgct tgaatggcag caataaatta gacagacaca cccaggccac 1621 cactcttgtt tgtcagatat ttggaggata cctaagatct agagtcaaat gtttaaattg 1681 caagggcgtt tcagatactt ttgatccata tcttgatata acattggaga taaaggctgc 1741 tcagagtgtc aacaaggcat tggagcagtt tgtgaagccg gaacagcttg atggagaaaa 1801 ctcgtacaag tgcagcaagt gtaaaaagat ggttccagct tcaaagaggt tcactatcca 1861 tagatcctct aatgttctta cactttctct gaaacgtttt gcaaatttta ccggtggaaa 1921 aattgctaag gatgtgaaat accctgagta tcttgatatt cggccatata tgtctcaacc 1981 caacggagag ccaattgtct acgtcttgta tgcagtgctg gtccacactg gttttaattg 2041 ccatgctggc cattacttct gctacataaa agctagcaat ggcctctggt atcaaatgaa 2101 tgactccatt gtatctacca gtgatattag atcggtactc agccaacaag cctatgtgct 2161 cttttatatc aggtcccatg atgtgaaaaa tggaggtgaa cttactcatc ccacccatag 2221 ccccggccag tcctctcccc gccccgtcat cagtcagcgg gttgtcacca acaaacaggc 2281 tgcgccaggc tttatcggac cacagcttcc ctctcacatg ataaagaatc cacctcactt 2341 aaatgggact ggaccattga aagacacgcc aagcagttcc atgtcgagtc ctaacgggaa 2401 ttccagtgtc aacagggcta gtcctgttaa tgcttcagct tctgtccaaa actggtcagt 2461 taataggtcc tcagtgatcc cagaacatcc taagaaacaa aaaattacaa tcagtattca 2521 caacaagttg cctgttcgcc agtgtcagtc tcaacctaac cttcatagta attctttgga 2581 gaaccctacc aagcccgttc cctcttctac cattaccaat tctgcagtac agtctacctc 2641 gaacgcatct acgatgtcag tttctagtaa agtaacaaaa ccgatccccc gcagtgaatc 2701 ctgctcccag cccgtgatga atggcaaatc caagctgaac tccagcgtgc tggtgcccta 2761 tggcgccgag tcctctgagg actctgacga ggagtcaaag gggctgggca aggagaatgg 2821 gattggtacg attgtgagct cccactctcc cggccaagat gccgaagatg aggaggccac 2881 tccgcacgag cttcaagaac ccatgaccct aaacggtgct aatagtgcag acagcgacag 2941 tgacccgaaa gaaaacggcc tagcgcctga tggtgccagc tgccaaggcc agcctgccct 3001 gcactcagaa aatccctttg ctaaggcaaa cggtcttcct ggaaagttga tgcctgctcc 3061 tttgctgtct ctcccagaag acaaaatctt agagaccttc aggcttagca acaaactgaa 3121 aggctcgacg gatgaaatga gtgcacctgg agcagagagg ggccctcccg aggaccgcga 3181 cgccgagcct cagcctggca gccccgccgc cgaatccctg gaggagccag atgcggccgc 3241 cggcctcagc agcaccaaga aggctccgcc gccccgcgat cccggcaccc ccgctaccaa 3301 agaaggcgcc tgggaggcca tggccgtcgc ccccgaggag cctccgccca gcgccggcga 3361 ggacatcgtg ggggacacag caccccctga cctgtgtgat cccgggagct taacaggcga 3421 tgcgagcccg ttgtcccagg acgcaaaggg gatgatcgcg gagggcccgc gggactcggc 3481 gttggcggaa gccccggaag ggttgagtcc ggctccgcct gcgcggtcgg aggagccctg 3541 cgagcagcca ctccttgttc accccagcgg ggaccacgcc cgggacgctc aggacccatc 3601 ccagagcttg ggcgcacccg aggccgcaga gcggccgcca gctcctgtgc tggacatggc 3661 cccggccggt cacccggaag gggacgctga gcctagcccc ggcgagaggg tcgaggacgc 3721 cgcggcgccg aaagccccag gcccttcccc agcgaaggag aaaatcggca gcctcagaaa 3781 ggtggaccga ggccactacc gcagccggag agagcgctcg tccagcgggg agcccgccag 3841 agagagcagg agcaagactg agggccaccg tcaccggcgg cgccgcacct gcccccggga 3901 gcgcgaccgc caggaccgcc acgccccgga gcaccacccc ggccacggcg acaggctcag 3961 ccctggcgag cgccgctctc tgggcaggtg cagtcaccac cactcccgac accggagcgg 4021 ggtggagctg gactgggtca gacaccacta caccgagggc gagcgtggct ggggccggga 4081 gaagttctac cccgacaggc cgcgctggga caggtgccgg tactaccatg acaggtacgc 4141 cctgtacgct gcccgggact ggaagccctt ccacggcggc cgcgagcacg agcgggccgg 4201 gctgcacgag cggccgcaca aggaccacaa ccggggccgt aggggctgcg agccggcccg 4261 ggagagggag cggcaccgcc ccagcagccc ccgcgcaggc gcgccccacg ccctcgcccc 4321 gcaccccgac cgcttctccc acgacagaac tgcacttgta gccggagaca actgtaacct 4381 ctctgatcgg tttcacgaac acgaaaatgg aaagtcccgg aaacggagac acgacagtgt 4441 ggagaacagt gacagtcatg ttgaaaagaa agcccggagg agcgaacaga aggatcctct 4501 agaagagcct aaagcaaaga agcacaaaaa atcaaagaag aaaaagaaat ccaaagacaa 4561 acaccgagac cgcgactcca ggcatcagca ggactcagac ctctcagcag cgtgctctga 4621 cgctgacctc cacagacaca aaaaaaagaa gaagaaaaag aagagacatt caagaaaatc 4681 agaggacttt gttaaagatt cagaactgca cttacccagg gtcaccagct tggagactgt 4741 cgcccagttc cggagagccc agggtggctt tcctctctct ggtggcccgc ctctggaagg 4801 cgtcggacct ttccgtgaga aaacgaaaca cttacggatg gaaagcaggg atgacaggtg 4861 tcgtctcttt gagtatggcc agggtgatct cgaggattac aaggatgacg acgataagta 4921 gtgagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 4981 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 5041 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 5101 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 5161 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 5221 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 5281 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 5341 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 5401 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 5461 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 5521 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 5581 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 5641 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 5701 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga 5761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 5821 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat 5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc 5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag 6001 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt 6061 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 6121 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 6181 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 6241 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 6301 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6361 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6421 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6481 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6541 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 6601 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 6661 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 6721 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 6781 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 6841 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 6901 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 6961 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 7021 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 7081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 7141 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 7201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 7261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 7321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 7381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 7441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 7501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 7561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 7621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 7681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 7741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 7801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 7861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 7921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 7981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 8041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 8101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc 8161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc //