LOCUS       V025615                 8186 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025615
VERSION     V025615
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3673
                     /label="Stat2(NM_019963)"
                     /note="Stat2(NM_019963)"
                     /gene="Stat2"
     polyA_signal    3753..3977
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4023..4451
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4465..4794
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4861..5655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5829..5950
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5999..6015)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6023..6039
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6047..6077)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6092..6113
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6401..6986)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7157..8017)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8018..8122)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcgcagtggg agatgttgca gaaccttgac agcctgttcc tggaccagtt
      961 acaccaggtc tactcacaga gcatcctgcc aatggacgtt cgacagcatt tggctacctg
     1021 gattgaagac cagaactgga gggaagctgc gctgggcagt gatgacgcca aggccaacat
     1081 gctatacttc agcatcctgg accaactgaa ccagtgggac cactacagct cagactccaa
     1141 tcacttttta ttacagcata acttgcgaaa attcagccgg gatattcaga cctttcccaa
     1201 tggccctacc cagttggctg agatgatctt taatcttctt ctggaagagc aaaggattct
     1261 gaatcaggct caaagagctc aagaggtgca gcccccacca gcccccgaag cagttgtgga
     1321 gagccagcag cttgagattg aaaatcgaat ccagggttta catgtggaca ttgagttctt
     1381 ggtgagatcc atcaggcagc tgaaggacga acaggatgtc ttcagcttca gatacacagt
     1441 tttcagtctg aagaagacgt cgtcttcaga cccccatcag agccaacagg cgcagcttgt
     1501 gcaggcaaca gccaacaaag tcgacagaat gagaaaggag gtgctggaca tctccaaagg
     1561 actggttggc cgattaacca ccctggtcga cctattgctg cccaagctgg acgagtggaa
     1621 ggtgcagcag cagaagtcct gcattggagc cccgcccccc gagctgcagc tggaacagct
     1681 ggaacagtgg ttgacggctg gagcaaagtt cttgttccac cttcggcagc tactgaagca
     1741 gctgaaggag atgagtcaca tgcttcggta taagggtgac atgtttggcc aaggggtgga
     1801 cctgcagaat gcccaagtca tggagttact tcagcgtctg ctccaaaggt cctttgtagt
     1861 agaaacccag ccctgcatgc cccagactct ccaccgaccc ctcatcctga agactgggaa
     1921 caagttcacc gtccgaacaa gacttctggt gagactccag gaaggcagcg aatcactcaa
     1981 agcagaagtc tctgtcgaca ggaattcaga cttaccaggc ttccggaagt tcaacattct
     2041 gacctcaaac caaaaaacct tgaccccgga ggagggccag agacagggct taatttggga
     2101 cttcggcttc ttgactctgg tggagcaacg cgctgtgggt gcaggaaagg gcaacaaggg
     2161 gccgctggca gtgacagagg agttacatgt catcagcttc gtggttgagt acgtgtacca
     2221 gggtctgaag atgaagctgc agacggacac tctccctgtt gtgattattt ctaacatgaa
     2281 ccaactctcc attgcctggg cctccattct ctggttcaac atgctcagcc caaatcccaa
     2341 gaaccagcag ttcttctgcc aagctccgaa agccccctgg agtttgctgg gacctgtgct
     2401 cagctggcag ttctcctctt acgttggccg aggcctcgat tctgagcagc tgggcatgct
     2461 gagaaccaag ctgtttggaa agagctgcaa gatggaggat gcgctgttgt cctgggtaga
     2521 cttttgcaag cgagagagcc cccctggtaa gatccctttc tggacctggc tggacaaaat
     2581 tctggagctg gtgcacgacc acctgaagga tctctggaag gatgggcgca tcatgggctt
     2641 tgtgagccgc aaccaggaac gcagactgct gaagaagatg ttgtctggca ccttcctact
     2701 gcgcttcagt gaaacttctg aagggggcat tacttgttct tgggtggagc accaggatga
     2761 cgataaagtc gaaatctact cagtgcagcc ctacaccaag gaagtgttac agtcactccc
     2821 actgacagag atcatccgcc actaccaggt tcttgccgaa gagaacatcc ccgagaaccc
     2881 actccgcttc ctctatcccc gaatccctcg ggacgaagct tttgggtgtt actaccagga
     2941 aaaagttaat tttgaagaac agaggaaata tttgaaacat aaactcattg tgatctctaa
     3001 cagacaggtg gacgagctgc agcagcctct ggagctcaaa caggattcag agtccttaga
     3061 agtgaatgca gagctcttgt tagcacacga ccaggagttg ccattgatga tgcagactgg
     3121 gctggttctg ggcacagagc tgaaagtgga ccccatactg agtacagccc cacaagtcct
     3181 gctggagcca gccccacaag tcctgctgga gccagcccca caagtcccgc tggagccagc
     3241 cccacaagtc ctgctggagc cagccccaca agtcctgctg gagccagccc cacaagtcct
     3301 gctggagcca gccccacaag tcctgctgga gccagcccca caagtccagc tggagccagc
     3361 cccacaagtc ctgctggagc tagccccaca agtcctgctg gagccagccc cacaagtcct
     3421 gctggagcta gccccacaag tccagctgga gccagcacac ttgctgcagc agccatcaga
     3481 gtcagatttg cctgaggacc tgcagcagat tagcgtggag gatctgaaaa aattaagcaa
     3541 cccgagtact gaatacatca caaccaacga aaacccaatg ttggctggcg agagcagtgg
     3601 agatgagact tccataccct accacagcca ttttgatgcg gatggactct tgggatggac
     3661 tcttgatacc ttctgagaat tctgcagata tccagcacag tggcggccgc tcgagtctag
     3721 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3781 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3841 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3901 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     3961 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     4021 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4081 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4141 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4201 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4261 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4321 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4381 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4441 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     4501 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     4561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     4621 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     4681 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     4741 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     4801 ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc
     4861 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     4921 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     4981 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     5041 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     5101 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     5161 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     5221 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     5281 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     5341 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     5401 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     5461 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     5521 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     5581 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     5641 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     5701 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     5761 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     5821 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5881 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5941 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt
     6001 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     6061 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     6121 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     6181 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6241 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6301 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6361 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6421 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6481 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6541 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6601 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6661 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6721 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6781 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6841 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6901 gctcttgatc cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga
     6961 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7021 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7081 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7141 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7201 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7261 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7321 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7381 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7441 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7501 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7561 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7621 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7681 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7741 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7801 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7861 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7921 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7981 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8041 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8101 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8161 cgggagatct cccgatcccc tatggt
//
