LOCUS       V025060                 7955 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025060
VERSION     V025060
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3448
                     /label="STAT6(NM_003153)"
                     /note="STAT6(NM_003153)"
                     /gene="STAT6"
     CDS             3455..3484
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    3522..3746
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3792..4220
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4234..4563
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4630..5424
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5598..5719
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5768..5784)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5792..5808
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5816..5846)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5861..5882
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6170..6755)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6926..7786)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7787..7891)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg tctctgtggg gtctggtctc caagatgccc ccagaaaaag tgcagcggct
      961 ctatgtcgac tttccccaac acctgcggca tcttctgggt gactggctgg agagccagcc
     1021 ctgggagttc ctggtcggct ccgacgcctt ctgctgcaac ttggctagtg ccctactttc
     1081 agacactgtc cagcaccttc aggcctcggt gggagagcag ggggagggga gcaccatctt
     1141 gcaacacatc agcacccttg agagcatata tcagagggac cccctgaagc tggtggccac
     1201 tttcagacaa atacttcaag gagagaaaaa agctgttatg gaacagttcc gccacttgcc
     1261 aatgcctttc cactggaagc aggaagaact caagtttaag acaggcttgc ggaggctgca
     1321 gcaccgagta ggggagatcc accttctccg agaagccctg cagaaggggg ctgaggctgg
     1381 ccaagtgtct ctgcacagct tgatagaaac tcctgctaat gggactgggc caagtgaggc
     1441 cctggccatg ctactgcagg agaccactgg agagctagag gcagccaaag ccctagtgct
     1501 gaagaggatc cagatttgga aacggcagca gcagctggca gggaatggcg caccgtttga
     1561 ggagagcctg gccccactcc aggagaggtg tgaaagcctg gtggacattt attcccagct
     1621 acagcaggag gtaggggcgg ctggtgggga gcttgagccc aagacccggg catcgctgac
     1681 tggccggctg gatgaagtcc tgagaaccct cgtcaccagt tgcttcctgg tggagaagca
     1741 gcccccccag gtactgaaga ctcagaccaa gttccaggct ggagttcgat tcctgttggg
     1801 cttgaggttc ctgggggccc cagccaagcc tccgctggtc agggccgaca tggtgacaga
     1861 gaagcaggcg cgggagctga gtgtgcctca gggtcctggg gctggagcag aaagcactgg
     1921 agaaatcatc aacaacactg tgcccttgga gaacagcatt cctgggaact gctgctctgc
     1981 cctgttcaag aacctgcttc tcaagaagat caagcggtgt gagcggaagg gcactgagtc
     2041 tgtcacagag gagaagtgcg ctgtgctctt ctctgccagc ttcacacttg gccccggcaa
     2101 actccccatc cagctccagg ccctgtctct gcccctggtg gtcatcgtcc atggcaacca
     2161 agacaacaat gccaaagcca ctatcctgtg ggacaatgcc ttctctgaga tggaccgcgt
     2221 gccctttgtg gtggctgagc gggtgccctg ggagaagatg tgtgaaactc tgaacctgaa
     2281 gttcatggct gaggtgggga ccaaccgggg gctgctccca gagcacttcc tcttcctggc
     2341 ccagaagatc ttcaatgaca acagcctcag tatggaggcc ttccagcacc gttctgtgtc
     2401 ctggtcgcag ttcaacaagg agatcctgct gggccgtggc ttcacctttt ggcagtggtt
     2461 tgatggtgtc ctggacctca ccaaacgctg tctccggagc tactggtctg accggctgat
     2521 cattggcttc atcagcaaac agtacgttac tagccttctt ctcaatgagc ccgacggaac
     2581 ctttctcctc cgcttcagcg actcagagat tgggggcatc accattgccc atgtcatccg
     2641 gggccaggat ggctctccac agatagagaa catccagcca ttctctgcca aagacctgtc
     2701 cattcgctca ctgggggacc gaatccggga tcttgctcag ctcaaaaatc tctatcccaa
     2761 gaagcccaag gatgaggctt tccggagcca ctacaagcct gaacagatgg gtaaggatgg
     2821 caggggttat gtcccagcta ccatcaagat gaccgtggaa agggaccaac cacttcctac
     2881 cccagagctc cagatgccta ccatggtgcc ttcttatgac cttggaatgg cccctgattc
     2941 ctccatgagc atgcagcttg gcccagatat ggtgccccag gtgtacccac cacactctca
     3001 ctccatcccc ccgtatcaag gcctctcccc agaagaatca gtcaacgtgt tgtcagcctt
     3061 ccaggagcct cacctgcaga tgccccccag cctgggccag atgagcctgc cctttgacca
     3121 gcctcacccc cagggcctgc tgccgtgcca gcctcaggag catgctgtgt ccagccctga
     3181 ccccctgctc tgctcagatg tgaccatggt ggaagacagc tgcctgagcc agccagtgac
     3241 agcgtttcct cagggcactt ggattggtga agacatattc cctcctctgc tgcctcccac
     3301 tgaacaggac ctcactaagc ttctcctgga ggggcaaggg gagtcggggg gagggtcctt
     3361 gggggcacag cccctcctgc agccctccca ctatgggcaa tctgggatct caatgtccca
     3421 catggaccta agggccaacc ccagttggct cgaggagcag aagctgatct cagaggagga
     3481 cctgtagtga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3541 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3601 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3661 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3721 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3781 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3841 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3901 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3961 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4021 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4081 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4141 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4201 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4321 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4381 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4441 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4501 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4561 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     4621 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     4681 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     4741 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     4801 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     4861 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     4921 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     4981 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5041 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5101 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5161 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5221 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5281 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5341 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5401 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     5461 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     5521 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     5581 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     5641 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     5701 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     5761 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5821 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5881 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     5941 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6001 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6061 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6121 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6181 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6241 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6301 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6361 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6421 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6481 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     6541 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6601 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6661 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     6721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6781 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     7921 tcgacggatc gggagatctc ccgatcccct atggt
//
