LOCUS V020410 5822 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020410 VERSION V020410 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 908..1315 /label="SRP14(NM_003134)" /note="SRP14(NM_003134)" /gene="SRP14" CDS 1325..1342 /codon_start=1 /product="6xHis affinity tag" /transl_table=1 /translation="HHHHHH" /label="6xHis" polyA_signal 1389..1613 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1659..2087 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2101..2430 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2497..3291 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3465..3586 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3635..3651) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3659..3675 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3683..3713) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3728..3749 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4037..4622) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4793..5653) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5654..5758) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cgccaccatg gtgttgttgg agagcgagca gttcctgacg gagctgacca gacttttcca 961 gaagtgccgg acgtcgggca gcgtctatat caccttgaag aagtatgacg gtcgaaccaa 1021 acccattcca aagaagggta ctgtggaggg ctttgagccc gcagacaaca agtgtctgtt 1081 aagagctacc gatgggaaga agaagatcag cactgtggtg agctccaagg aagtgaataa 1141 gtttcagatg gcttattcaa acctccttag agctaacatg gatgggctga agaagagaga 1201 caaaaagaac aaaactaaga agaccaaagc agcagcagca gcagcagcag cagcacctgc 1261 cgcagcagca acagcaccaa caacagcagc aacaacagca gcaacagcag cacaggcggc 1321 cgcacatcac catcatcacc attgactcga gtctagaggg cccgtttaaa cccgctgatc 1381 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 1441 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 1501 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 1561 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga 1621 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt 1681 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 1741 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 1801 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 1861 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 1921 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 1981 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc 2041 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat 2101 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc 2161 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga 2221 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc 2281 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 2341 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga 2401 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc 2461 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac 2521 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca 2581 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt 2641 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg 2701 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga 2761 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct 2821 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg 2881 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg 2941 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc 3001 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat 3061 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac 3121 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt 3181 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct 3241 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc 3301 tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat ttcgattcca 3361 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 3421 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 3481 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 3541 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 3601 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 3661 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 3721 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 3781 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 3841 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3901 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3961 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4021 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4081 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4141 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4201 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4261 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4321 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 4381 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 4441 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 4501 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4561 ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4621 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4681 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 4741 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 4801 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 4861 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 4921 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 4981 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 5041 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 5101 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 5161 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 5221 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 5281 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 5341 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 5401 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 5461 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 5521 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 5581 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 5641 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 5701 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 5761 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg 5821 gt //