LOCUS       V021064                 6976 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V021064
VERSION     V021064
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             896..2434
                     /label="SPINT1(NM_003710)"
                     /note="SPINT1(NM_003710)"
                     /gene="SPINT1"
     CDS             2435..2464
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /transl_table=1
                     /translation="EQKLISEEDL"
                     /label="Myc"
     polyA_signal    2543..2767
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      2813..3241
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3255..3584
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             3651..4445
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    4619..4740
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(4789..4805)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    4813..4829
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(4837..4867)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    4882..4903
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5191..5776)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(5947..6807)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(6808..6912)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg ccaccatggc
      901 ccctgcgagg acgatggccc gcgcccgcct cgccccggcc ggcatccctg ccgtcgcctt
      961 gtggcttctg tgcacgctcg gcctccaggg cacccaggcc gggccaccgc ccgcgccccc
     1021 tgggctgccc gcgggagccg actgcctgaa cagctttacc gccggggtgc ctggcttcgt
     1081 gctggacacc aacgcctcgg tcagcaacgg agctaccttc ctggagtccc ccaccgtgcg
     1141 ccggggctgg gactgcgtgc gcgcctgctg caccacccag aactgcaact tggcgctagt
     1201 ggagctgcag cccgaccgcg gggaggacgc catcgccgcc tgcttcctca tcaactgcct
     1261 ctacgagcag aacttcgtgt gcaagttcgc gcccagggag ggcttcatca actacctcac
     1321 gagggaagtg taccgctcct accgccagct gcggacccag ggctttggag ggtctgggat
     1381 ccccaaggcc tgggcaggca tagacttgaa ggtacaaccc caggaacccc tggtgctgaa
     1441 ggatgtggaa aacacagatt ggcgcctact gcggggtgac acggatgtca gggtagagag
     1501 gaaagaccca aaccaggtgg aactgtgggg actcaaggaa ggcacctacc tgttccagct
     1561 gacagtgact agctcagacc acccagagga cacggccaac gtcacagtca ctgtgctgtc
     1621 caccaagcag acagaagact actgcctcgc atccaacaag gtgggtcgct gccggggctc
     1681 tttcccacgc tggtactatg accccacgga gcagatctgc aagagtttcg tttatggagg
     1741 ctgcttgggc aacaagaaca actaccttcg ggaagaagag tgcattctag cctgtcgggg
     1801 tgtgcaaggc ccctccatgg aaaggcgcca tccagtgtgc tctggcacct gtcagcccac
     1861 ccagttccgc tgcagcaatg gctgctgcat cgacagtttc ctggagtgtg acgacacccc
     1921 caactgcccc gacgcctccg acgaggctgc ctgtgaaaaa tacacgagtg gctttgacga
     1981 gctccagcgc atccatttcc ccagtgacaa agggcactgc gtggacctgc cagacacagg
     2041 actctgcaag gagagcatcc cgcgctggta ctacaacccc ttcagcgaac actgcgcccg
     2101 ctttacctat ggtggttgtt acggcaacaa gaacaacttt gaggaagagc agcagtgcct
     2161 cgagtcttgt cgcggcatct ccaagaagga tgtgtttggc ctgaggcggg aaatccccat
     2221 tcccagcaca ggctctgtgg agatggctgt cgcagtgttc ctggtcatct gcattgtggt
     2281 ggtggtagcc atcttgggtt actgcttctt caagaaccag agaaaggact tccacggaca
     2341 ccaccaccac ccaccaccca cccctgccag ctccactgtc tccactaccg aggacacgga
     2401 gcacctggtc tataaccaca ccacgcggcc cctcgagcaa aagctcattt ctgaagagga
     2461 cttgtagaat tctgcagata tccagcacag tggcggccgc tcgagtctag agggcccgtt
     2521 taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2581 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2641 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     2701 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     2761 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc
     2821 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     2881 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     2941 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     3001 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     3061 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     3121 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     3181 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     3241 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     3301 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     3361 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     3421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc
     3541 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt
     3601 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
     3661 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     3721 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     3781 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
     3841 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     3901 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     3961 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     4021 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     4081 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     4141 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
     4201 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     4261 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     4321 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     4381 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     4441 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     4501 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     4561 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     4621 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     4681 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4741 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     4801 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4861 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4921 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5701 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag
     5761 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     5821 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     5881 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     5941 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6001 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6061 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6121 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6181 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6241 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6301 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6361 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     6421 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     6481 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     6541 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     6601 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     6661 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     6721 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     6781 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     6841 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6901 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct
     6961 cccgatcccc tatggt
//
