LOCUS       V018427                12094 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018427
VERSION     V018427
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             932..7573
                     /label="SORL1(NM_003105)"
                     /note="SORL1(NM_003105)"
                     /gene="SORL1"
     CDS             7595..7660
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     polyA_signal    7689..7913
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      7959..8387
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        8401..8730
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             8797..9591
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    9767..9888
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(9937..9953)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    9961..9977
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(9985..10015)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    10030..10051
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(10339..10927)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(11098..11958)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(11959..12063)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccgccac catggcgaca cggagcagca ggagggagtc
      961 gcgactcccg ttcctattca ccctggtcgc actgctgccg cccggagctc tctgcgaagt
     1021 ctggacgcag aggctgcacg gcggcagcgc gcccttgccc caggaccggg gcttcctcgt
     1081 ggtgcagggc gacccgcgcg agctgcggct gtgggcgcgc ggggatgcca ggggggcgag
     1141 ccgcgcggac gagaagccgc tccggaggaa acggagcgct gccctgcagc ccgagcccat
     1201 caaggtgtac ggacaggtta gtctgaatga ttcccacaat cagatggtgg tgcactgggc
     1261 tggagagaaa agcaacgtga tcgtggcctt ggcccgagat agcctggcat tggcgaggcc
     1321 caagagcagt gatgtgtacg tgtcttacga ctatggaaaa tcattcaaga aaatttcaga
     1381 caagttaaac tttggcttgg gaaataggag tgaagctgtt atcgcccagt tctaccacag
     1441 ccctgcggac aacaagcggt acatctttgc agacgcttat gcccagtacc tctggatcac
     1501 gtttgacttc tgcaacactc ttcaaggctt ttccatccca tttcgggcag ctgatctcct
     1561 cctacacagt aaggcctcca accttctctt gggctttgac aggtcccacc ccaacaagca
     1621 gctgtggaag tcagatgact ttggccagac ctggatcatg attcaggaac atgtcaagtc
     1681 cttttcttgg ggaattgatc cctatgacaa accaaatacc atctacattg aacgacatga
     1741 accctctggc tactccactg tcttccgaag tacagatttc ttccagtccc gggaaaacca
     1801 ggaagtgatc cttgaggaag tgagagattt tcagcttcgg gacaagtaca tgtttgctac
     1861 aaaggtggtg catctcttgg gcagtgaaca gcagtcttct gtccagctct gggtctcctt
     1921 tggccggaag cccatgagag cagcccagtt tgtcacaaga catcctatta atgaatatta
     1981 catcgcagat gcctccgagg accaggtgtt tgtgtgtgtc agccacagta acaaccgcac
     2041 caatttatac atctcagagg cagaggggct gaagttctcc ctgtccttgg agaacgtgct
     2101 ctattacagc ccaggagggg ccggcagtga caccttggtg aggtattttg caaatgaacc
     2161 atttgctgac ttccaccgag tggaaggatt gcaaggagtc tacattgcta ctctgattaa
     2221 tggttctatg aatgaggaga acatgagatc ggtcatcacc tttgacaaag ggggaacctg
     2281 ggagtttctt caggctccag ccttcacggg atatggagag aaaatcaatt gtgagctttc
     2341 ccagggctgt tcccttcatc tggctcagcg cctcagtcag ctcctcaacc tccagctccg
     2401 gagaatgccc atcctgtcca aggagtcggc tccaggcctc atcatcgcca ctggctcagt
     2461 gggaaagaac ttggctagca agacaaacgt gtacatctct agcagtgctg gagccaggtg
     2521 gcgagaggca cttcctggac ctcactacta cacatgggga gaccacggcg gaatcatcac
     2581 ggccattgcc cagggcatgg aaaccaacga gctaaaatac agtaccaatg aaggggagac
     2641 ctggaaaaca ttcatcttct ctgagaagcc agtgtttgtg tatggcctcc tcacagaacc
     2701 tggggagaag agcactgtct tcaccatctt tggctcgaac aaagagaatg tccacagctg
     2761 gctgatcctc caggtcaatg ccacggatgc cttgggagtt ccctgcacag agaatgacta
     2821 caagctgtgg tcaccatctg atgagcgggg gaatgagtgt ttgctgggac acaagactgt
     2881 tttcaaacgg cggacccccc atgccacatg cttcaatgga gaggactttg acaggccggt
     2941 ggtcgtgtcc aactgctcct gcacccggga ggactatgag tgtgacttcg gtttcaagat
     3001 gagtgaagat ttgtcattag aggtttgtgt tccagatccg gaattttctg gaaagtcata
     3061 ctcccctcct gtgccttgcc ctgtgggttc tacttacagg agaacgagag gctaccggaa
     3121 gatttctggg gacacttgta gcggaggaga tgttgaagcg cgactggaag gagagctggt
     3181 cccctgtccc ctggcagaag agaacgagtt cattctgtat gctgtgagga aatccatcta
     3241 ccgctatgac ctggcctcgg gagccaccga gcagttgcct ctcaccgggc tacgggcagc
     3301 agtggccctg gactttgact atgagcacaa ctgtttgtat tggtccgacc tggccttgga
     3361 cgtcatccag cgcctttgtt tgaatggaag cacagggcaa gaggtgatca tcaattctgg
     3421 cctggagaca gtagaagctt tggcttttga acccctcagc cagctgcttt actgggtaga
     3481 tgcaggcttc aaaaagattg aggtagctaa tccagatggc gacttccgac tcacaatcgt
     3541 caattcctct gtgcttgatc gtcccagggc tctggtcctc gtgccccaag agggggtgat
     3601 gttctggaca gactggggag acctgaagcc tgggatttat cggagcaata tggatggttc
     3661 tgctgcctat cacctggtgt ctgaggatgt gaagtggccc aatggcatct ctgtggacga
     3721 ccagtggatt tactggacgg atgcctacct ggagtgcata gagcggatca cgttcagtgg
     3781 ccagcagcgc tctgtcattc tggacaacct cccgcacccc tatgccattg ctgtctttaa
     3841 gaatgaaatc tactgggatg actggtcaca gctcagcata ttccgagctt ccaaatacag
     3901 tgggtcccag atggagattc tggcaaacca gctcacgggg ctcatggaca tgaagatttt
     3961 ctacaagggg aagaacactg gaagcaatgc ctgtgtgccc aggccatgca gcctgctgtg
     4021 cctgcccaag gccaacaaca gtagaagctg caggtgtcca gaggatgtgt ccagcagtgt
     4081 gcttccatca ggggacctga tgtgtgactg ccctcagggc tatcagctca agaacaatac
     4141 ctgtgtcaaa caagagaaca cctgtcttcg caaccagtat cgctgcagca acgggaactg
     4201 tatcaacagc atttggtggt gtgactttga caacgactgt ggagacatga gcgatgagag
     4261 aaactgccct accaccatct gtgacctgga cacccagttt cgttgccagg agtctgggac
     4321 ttgtatccca ctgtcctata aatgtgacct tgaggatgac tgtggagaca acagtgatga
     4381 aagtcattgt gaaatgcacc agtgccggag tgacgagtac aactgcagtt ccggcatgtg
     4441 catccgctcc tcctgggtat gtgacgggga caacgactgc agggactggt ctgatgaagc
     4501 caactgtacc gccatctatc acacctgtga ggcctccaac ttccagtgcc gaaacgggca
     4561 ctgcatcccc cagcggtggg cgtgtgacgg ggatacggac tgccaggatg gttccgatga
     4621 ggatccagtc aactgtgaga agaagtgcaa tggattccgc tgcccaaatg gcacttgcat
     4681 cccatccagc aaacattgtg atggtctgcg tgattgctct gatggctccg atgaacagca
     4741 ctgcgagccc ctctgtacgc acttcatgga ctttgtgtgt aagaaccgcc agcagtgcct
     4801 gttccactcc atggtctgtg acggaatcat ccagtgccgc gacgggtccg atgaggatgc
     4861 ggcgtttgca ggatgctccc aagatcctga gttccacaag gtatgtgatg agttcggttt
     4921 ccagtgtcag aatggagtgt gcatcagttt gatttggaag tgcgacggga tggatgattg
     4981 cggcgattat tctgatgaag ccaactgcga aaaccccaca gaagccccaa actgctcccg
     5041 ctacttccag tttcggtgtg agaatggcca ctgcatcccc aacagatgga aatgtgacag
     5101 ggagaacgac tgtggggact ggtctgatga gaaggattgt ggagattcac atattcttcc
     5161 cttctcgact cctgggccct ccacgtgtct gcccaattac taccgctgca gcagtgggac
     5221 ctgcgtgatg gacacctggg tgtgcgacgg gtaccgagat tgtgcagatg gctctgacga
     5281 ggaagcctgc cccttgcttg caaacgtcac tgctgcctcc actcccaccc aacttgggcg
     5341 atgtgaccga tttgagttcg aatgccacca accgaagacg tgtattccca actggaagcg
     5401 ctgtgacggc caccaagatt gccaggatgg ccgggacgag gccaattgcc ccacacacag
     5461 caccttgact tgcatgagca gggagttcca gtgcgaggac ggggaggcct gcattgtgct
     5521 ctcggagcgc tgcgacggct tcctggactg ctcggacgag agcgatgaaa aggcctgcag
     5581 tgatgagttg actgtgtaca aagtacagaa tcttcagtgg acagctgact tctctgggga
     5641 tgtgactttg acctggatga ggcccaaaaa aatgccctct gcttcttgtg tatataatgt
     5701 ctactacagg gtggttggag agagcatatg gaagactctg gagacccaca gcaataagac
     5761 aaacactgta ttaaaagtct tgaaaccaga taccacgtat caggttaaag tacaggttca
     5821 gtgtctcagc aaggcacaca acaccaatga ctttgtgacc ctgaggaccc cagagggatt
     5881 gccagatgcc cctcgaaatc tccagctgtc actccccagg gaagcagaag gtgtgattgt
     5941 aggccactgg gctcctccca tccacaccca tggcctcatc cgtgagtaca ttgtagaata
     6001 cagcaggagt ggttccaaga tgtgggcctc ccagagggct gctagtaact ttacagaaat
     6061 caagaactta ttggtcaaca ctctatacac cgtcagagtg gctgcggtga ctagtcgtgg
     6121 aataggaaac tggagcgatt ctaaatccat taccaccata aaaggaaaag tgatcccacc
     6181 accagatatc cacattgaca gctatggtga aaattatcta agcttcaccc tgaccatgga
     6241 gagtgatatc aaggtgaatg gctatgtggt gaaccttttc tgggcatttg acacccacaa
     6301 gcaagagagg agaactttga acttccgagg aagcatattg tcacacaaag ttggcaatct
     6361 gacagctcat acatcctatg agatttctgc ctgggccaag actgacttgg gggatagccc
     6421 tctggcattt gagcatgtta tgaccagagg ggttcgccca cctgcaccta gcctcaaggc
     6481 caaagccatc aaccagactg cagtggaatg tacctggacc ggcccccgga atgtggttta
     6541 tggtattttc tatgccacgt cctttcttga cctctatcgc aacccgaaga gcttgactac
     6601 ttcactccac aacaagacgg tcattgtcag taaggatgag cagtatttgt ttctggtccg
     6661 tgtagtggta ccctaccagg ggccatcctc tgactacgtt gtagtgaaga tgatcccgga
     6721 cagcaggctt ccaccccgtc acctgcatgt ggttcatacg ggcaaaacct ccgtggtcat
     6781 caagtgggaa tcaccgtatg actctcctga ccaggacttg ttgtatgcag ttgcagtcaa
     6841 agatctcata agaaagactg acaggagcta caaagtaaaa tcccgtaaca gcactgtgga
     6901 atacaccctt aacaagttgg agcctggcgg gaaataccac atcattgtcc aactggggaa
     6961 catgagcaaa gattccagca taaaaattac cacagtttca ttatcagcac ctgatgcctt
     7021 aaaaatcata acagaaaatg atcatgttct tctgttttgg aaaagcctgg ctttaaagga
     7081 aaagcatttt aatgaaagca ggggctatga gatacacatg tttgatagtg ccatgaatat
     7141 cacagcttac cttgggaata ctactgacaa tttctttaaa atttccaacc tgaagatggg
     7201 tcataattac acgttcaccg tccaagcaag atgccttttt ggcaaccaga tctgtgggga
     7261 gcctgccatc ctgctgtacg atgagctggg gtctggtgca gatgcatctg caacgcaggc
     7321 tgccagatct acggatgttg ctgctgtggt ggtgcccatc ttattcctga tactgctgag
     7381 cctgggggtg gggtttgcca tcctgtacac gaagcaccgg aggctgcaga gcagcttcac
     7441 cgccttcgcc aacagccact acagctccag gctggggtcc gcaatcttct cctctgggga
     7501 tgacctgggg gaagatgatg aagatgcccc tatgataact ggattttcag atgacgtccc
     7561 catggtgata gccctcgagt ctagagggcc cttcgactac aaagaccatg acggtgatta
     7621 taaagatcat gacatcgact acaaggatga cgatgacaag tgagtttaaa cccgctgatc
     7681 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     7741 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     7801 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     7861 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     7921 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     7981 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     8041 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     8101 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     8161 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     8221 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     8281 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     8341 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     8401 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     8461 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     8521 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     8581 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8641 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     8701 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     8761 ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     8821 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     8881 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8941 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
     9001 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9061 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9121 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9181 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9241 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9301 gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat
     9361 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9421 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     9481 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     9541 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcgggactc
     9601 tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     9661 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9721 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     9781 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9841 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9901 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9961 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10021 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10081 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10141 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10201 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10261 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10321 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10381 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10441 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10501 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10561 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10621 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10681 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10741 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    10801 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10861 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10921 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10981 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11041 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11101 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11161 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11221 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11281 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11341 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11401 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11461 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11521 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11581 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11641 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11701 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    11761 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    11821 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11881 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11941 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12001 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12061 gcgcacattt ccccgaaaag tgccacctga cgtc
//
