LOCUS       V038426                 7979 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038426
VERSION     V038426
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..2782
                     /label="SLC6A3(NM_001044)"
                     /note="SLC6A3(NM_001044)"
                     /gene="SLC6A3"
     CDS             2831..3544
                     /codon_start=1
                     /note="excitable with blue light"
                     /product="S65T variant of Aequorea victoria green
                     fluorescent protein (Heim et al., 1995)"
                     /transl_table=1
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
                     /label="GFP (S65T)"
     polyA_signal    3579..3803
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3849..4277
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4291..4620
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4687..5481
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5655..5776
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5825..5841)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5849..5865
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5873..5903)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5918..5939
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6227..6812)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6983..7843)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7844..7948)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgagtaa gagcaaatgc tccgtgggac tcatgtcttc
      961 cgtggtggcc ccggctaagg agcccaatgc cgtgggcccg aaggaggtgg agctcatcct
     1021 tgtcaaggag cagaacggag tgcagctcac cagctccacc ctcaccaacc cgcggcagag
     1081 ccccgtggag gcccaggatc gggagacctg gggcaagaag atcgactttc tcctgtccgt
     1141 cattggcttt gctgtggacc tggccaacgt ctggcggttc ccctacctgt gctacaaaaa
     1201 tggtggcggt gccttcctgg tcccctacct gctcttcatg gtcattgctg ggatgccact
     1261 tttctacatg gagctggccc tcggccagtt caacagggaa ggggccgctg gtgtctggaa
     1321 gatctgcccc atactgaaag gtgtgggctt cacggtcatc ctcatctcac tgtatgtcgg
     1381 cttcttctac aacgtcatca tcgcctgggc gctgcactat ctcttctcct ccttcaccac
     1441 ggagctcccc tggatccact gcaacaactc ctggaacagc cccaactgct cggatgccca
     1501 tcctggtgac tccagtggag acagctcggg cctcaacgac acttttggga ccacacctgc
     1561 tgccgagtac tttgaacgtg gcgtgctgca cctccaccag agccatggca tcgacgacct
     1621 ggggcctccg cggtggcagc tcacagcctg cctggtgctg gtcatcgtgc tgctctactt
     1681 cagcctctgg aagggcgtga agacctcagg gaaggtggta tggatcacag ccaccatgcc
     1741 atacgtggtc ctcactgccc tgctcctgcg tggggtcacc ctccctggag ccatagacgg
     1801 catcagagca tacctgagcg ttgacttcta ccggctctgc gaggcgtctg tttggattga
     1861 cgcggccacc caggtgtgct tctccctggg cgtggggttc ggggtgctga tcgccttctc
     1921 cagctacaac aagttcacca acaactgcta cagggacgcg attgtcacca cctccatcaa
     1981 ctccctgacg agcttctcct ccggcttcgt cgtcttctcc ttcctggggt acatggcaca
     2041 gaagcacagt gtgcccatcg gggacgtggc caaggacggg ccagggctga tcttcatcat
     2101 ctacccggaa gccatcgcca cgctccctct gtcctcagcc tgggccgtgg tcttcttcat
     2161 catgctgctc accctgggta tcgacagcgc catgggtggt atggagtcag tgatcaccgg
     2221 gctcatcgat gagttccagc tgctgcacag acaccgtgag ctcttcacgc tcttcatcgt
     2281 cctggcgacc ttcctcctgt ccctgttctg cgtcaccaac ggtggcatct acgtcttcac
     2341 gctcctggac cattttgcag ccggcacgtc catcctcttt ggagtgctca tcgaagccat
     2401 cggagtggcc tggttctatg gtgttgggca gttcagcgac gacatccagc agatgaccgg
     2461 gcagcggccc agcctgtact ggcggctgtg ctggaagctg gtcagcccct gctttctcct
     2521 gttcgtggtc gtggtcagca ttgtgacctt cagacccccc cactacggag cctacatctt
     2581 ccccgactgg gccaacgcgc tgggctgggt catcgccaca tcctccatgg ccatggtgcc
     2641 catctatgcg gcctacaagt tctgcagcct gcctgggtcc tttcgagaga aactggccta
     2701 cgccattgca cccgagaagg accgtgagct ggtggacaga ggggaggtgc gccagttcac
     2761 gctccgccac tggctcaagg tgcctcgagg cggcggaggg agtggaggag ggggaagcgg
     2821 atccgccacc atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
     2881 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
     2941 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
     3001 gccaacactt gtcactactt tcacttatgg tgttcaatgc ttttcaagat acccagatca
     3061 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
     3121 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
     3181 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
     3241 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca
     3301 aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca
     3361 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     3421 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     3481 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     3541 caaatagggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc
     3601 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     3661 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     3721 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     3781 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     3841 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3901 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3961 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4021 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4081 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4141 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4201 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4261 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     4321 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     4381 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     4441 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     4501 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     4561 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     4621 aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg
     4681 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     4741 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     4801 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     4861 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     4921 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     4981 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     5041 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     5101 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     5161 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     5221 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     5281 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     5341 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     5401 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     5461 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     5521 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     5581 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     5641 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     5701 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     5761 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     5821 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5881 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5941 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6001 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6061 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6121 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6181 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6241 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6301 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6361 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6421 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6481 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6541 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6601 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6661 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6721 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc
     6781 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6841 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6901 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6961 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7021 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7081 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7141 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7201 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7261 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7321 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7381 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7441 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7501 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7561 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7621 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7681 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7741 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7801 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7861 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7921 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
