LOCUS V024510 7776 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V024510 VERSION V024510 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 919..3297 /label="SF3A1(NM_005877)" /note="SF3A1(NM_005877)" /gene="SF3A1" promoter complement(3328..3346) /note="promoter for bacteriophage SP6 RNA polymerase" /label="SP6 promoter" polyA_signal 3372..3596 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3642..4070 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4084..4414 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4481..5275 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5449..5570 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5619..5635) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5643..5659 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5667..5697) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5712..5733 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6021..6609) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6780..7640) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7641..7745) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccgccaccat gccggccgga cccgtgcagg cggtgccccc gccgccgccc 961 gtgcccacgg agcccaaaca gcccacagaa gaagaagcat cttcaaagga ggattctgca 1021 ccttctaagc cagttgtggg gattatttac cctcctccag aggtcagaaa tattgttgac 1081 aagactgcca gctttgtggc cagaaacggg cctgaatttg aagctaggat ccgacagaac 1141 gagatcaaca accccaagtt caactttctg aaccccaatg acccttacca tgcctactac 1201 cgccacaagg tcagcgagtt caaggaaggg aaggctcagg agccgtccgc cgccatcccc 1261 aaggtcatgc agcagcagca gcagaccacc cagcagcagc tgccccagaa ggtccaagcc 1321 caagtaatcc aagagaccat cgtgcccaaa gagcctcctc ctgagtttga gttcattgct 1381 gatcctccct ctatctcagc cttcgacttg gatgtggtga agctgacggc tcagtttgtg 1441 gccaggaatg ggcgccagtt tctgacccag ctgatgcaga aagagcagcg caactaccag 1501 tttgactttc tccgcccaca gcacagcctc ttcaactact tcacgaagct agtggaacag 1561 tacaccaaga tcttgattcc acccaaaggt ttattttcaa agctcaagaa agaggctgaa 1621 aacccccgag aagttttgga tcaggtgtgt taccgagtgg aatgggccaa attccaggaa 1681 cgtgagagga agaaggaaga agaggagaag gagaaggagc gggtggccta tgctcagatc 1741 gactggcatg attttgtggt ggtggaaaca gtggacttcc aacccaatga gcaagggaac 1801 ttccctcccc ccaccacgcc agaggagctg ggggcccgaa tcctcattca ggagcgctat 1861 gaaaagtttg gggagagtga ggaagttgag atggaggtcg agtctgatga ggaggatgac 1921 aaacaggaga aggcggagga gcctccttcc cagctggacc aggacaccca agtacaagat 1981 atggatgagg gttcagatga tgaagaagaa gggcagaaag tgcccccacc cccagagaca 2041 cccatgcctc cacctctgcc cccaactcca gaccaagtca ttgtccgcaa ggattatgat 2101 cccaaagcct ccaagccctt gcctccagcc cctgctccag atgagtatct tgtgtccccc 2161 attactgggg agaagatccc cgccagcaaa atgcaggaac acatgcgcat tggacttctt 2221 gaccctcgct ggctggagca gcgggatcgc tccatccgtg agaagcagag cgatgatgag 2281 gtgtacgcac caggtctgga tattgagagc agcttgaagc agttggctga gcggcgtact 2341 gacatcttcg gtgtagagga aacagccatt ggtaagaaga tcggtgagga ggagatccag 2401 aagccagagg aaaaggtgac ctgggatggc cactcaggca gcatggcccg gacccagcag 2461 gctgcccagg ccaacatcac cctccaggag cagattgagg ccattcacaa ggccaaaggc 2521 ctggtgccag aggatgacac taaagagaag attggcccca gcaagcccaa tgaaatccct 2581 caacagccac cgccaccatc ttcagccacc aacatcccca gctcggctcc acccatcact 2641 tcagtgcccc gaccacccac aatgccacct ccagttcgta ctacagttgt ctccgcagta 2701 cccgtcatgc cccggccccc aatggcatct gtggtccggc tgcccccagg ctcagtgatc 2761 gcccccatgc cgcccatcat ccacgcgccc agaatcaacg tggtgcccat gcctccctcg 2821 gcccctccta ttatggcccc ccgcccaccc cccatgattg tgccaacagc ctttgtgcct 2881 gctccacctg tggcacctgt cccagctcca gccccaatgc cccctgtgca tcccccacct 2941 cccatggaag atgagcccac ctccaaaaaa ctgaagacag aggacagcct catgccagag 3001 gaggagttcc tgcgcagaaa caagggtcca gtgtccatca aagtccaggt gcccaacatg 3061 caggataaga cggaatggaa actgaatggg caggtgctgg tcttcaccct cccactcacg 3121 gaccaggtct ctgtcattaa ggtgaagatt catgaagcca caggcatgcc tgcagggaaa 3181 cagaagctac agtatgaggg tatcttcatc aaagattcca actcactggc ttactacaac 3241 atggccaatg gcgcagtcat ccacctggcc ctcaaggaga gaggcgggag gaagaagtag 3301 ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg 3361 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc 3421 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc 3481 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa 3541 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc 3601 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc 3661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 3721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 3781 tcaagctcta aatcggggca tccctttagg gttccgattt agtgctttac ggcacctcga 3841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 3901 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 3961 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tggggatttc 4021 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg 4081 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc caggcaggca gaagtatgca 4141 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg 4201 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc 4261 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 4321 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg 4381 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat 4441 tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt 4501 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca 4561 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct 4621 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct 4681 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc 4741 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct 4801 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga 4861 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg 4921 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc 4981 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac 5041 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat 5101 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga 5161 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc 5221 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg 5281 actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat 5341 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg 5401 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt 5461 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt 5521 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt 5581 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 5641 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 5701 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 5761 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 5821 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 5881 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 5941 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 6001 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 6061 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 6121 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 6181 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 6241 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 6301 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 6361 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 6421 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 6481 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 6541 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 6601 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 6661 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 6721 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 6781 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 6841 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 6901 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 6961 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 7021 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 7081 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 7141 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 7201 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 7261 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 7321 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 7381 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 7441 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 7501 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 7561 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 7621 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 7681 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 7741 ccgcgcacat ttccccgaaa agtgccacct gacgtc //