LOCUS       V032026                10220 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V032026
VERSION     V032026
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..5659
                     /label="SAMD9L(NM_152703)"
                     /note="SAMD9L(NM_152703)"
                     /gene="SAMD9L"
     CDS             5666..5692
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5696..5722
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             5726..5752
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    5787..6011
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      6057..6485
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        6499..6828
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6895..7689
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    7863..7984
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(8033..8049)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8057..8073
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8081..8111)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8126..8147
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8435..9020)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9191..10051)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10052..10156)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg agtaaacaag tatctctacc tgaaatgatt aaagactgga ccaaagagca
      961 tgtgaaaaaa tgggtaaatg aagaccttaa gattaatgag caatacgggc aaattctgct
     1021 cagtgaagaa gtaacaggat tagtcctgca ggaattaact gagaaggacc ttgtagaaat
     1081 ggggctacca tggggtccag cacttttgat aaaacgttca tacaacaaat tgaatagtaa
     1141 gtcccctgaa agtgacaatc atgatccggg acaattagat aattcaaaac cgtccaaaac
     1201 agaacaccag aaaaatccaa aacacaccaa aaaggaagaa gaaaattcaa tgtcatctaa
     1261 tattgattat gatcccagag agatcagaga tatcaaacaa gaagaatcaa ttcttatgaa
     1321 agaaaatgtg ttagatgaag tagcaaatgc taaacacaag aaaaagggta agctaaaacc
     1381 tgaacaattg acttgtatgc catatccttt tgatcagttc catgacagcc atcgctacat
     1441 agaacattat actctacaac ctgaaacagg agcactcaat ctcattgatc caatacatga
     1501 gttcaaagct ctcacaaaca cagaaacagc cacggaagtg gacattaaga tgaaattcag
     1561 caatgaagtc ttccgatttg catcagcttg tatgaattca cgcaccaatg gcaccatcca
     1621 ttttggagtc aaggacaaac cccatggaga aattgttggt gtgaaaatca ccagtaaggc
     1681 tgccttcatt gaccacttca atgtaatgat caaaaagtat tttgaagaaa gtgagatcaa
     1741 tgaagccaag aagtgtattc gggagccaag gtttgtggaa gtccttctgc agaacaatac
     1801 accatctgac agatttgtca ttgaagttga tactattcca aaacactcta tatgtaatga
     1861 taagtatttc tacattcaga tgcaaatttg taaagataaa atatggaaac aaaaccaaaa
     1921 tctttcactg tttgtaagag aaggggctag ctctagggat atcctggcca attccaagca
     1981 acgggatgta gatttcaagg catttttaca aaatttaaag tcactggtag catctagaaa
     2041 agaggctgaa gaagagtatg gaatgaaggc aatgaagaag gagagtgaag gactaaagct
     2101 ggttaaactt ctcataggaa accgagactc actggataat tcatactatg actggtacat
     2161 tcttgtaaca aataaatgcc atccaaacca aataaagcac ttagattttt taaaagaaat
     2221 taaatggttt gctgtgttgg agtttgatcc tgaatctatg atcaatggag tggtcaaagc
     2281 ttacaaagaa agtcgggtgg caaaccttca ctttccaaat caatatgaag acaagacaac
     2341 taacatgtgg gagaagattt ctactcttaa tctttaccaa cagcccagct ggattttctg
     2401 caacggcaga tcagacctga aaagcgagac atataaacct ctagaaccac atttatggca
     2461 gagagaaaga gcttcagaag tcaggaaact aattttattt ctcacagatg aaaatataat
     2521 gacaagagga aaatttttgg tagtgtttct attactctct tcagtggaaa gcccaggaga
     2581 tccactcatt gaaactttct gggctttcta tcaagctctc aaaggaatgg aaaatatgtt
     2641 gtgtatctct gtaaactcac atatttatca acgatggaaa gatctactac aaacaagaat
     2701 gaagatggaa gatgaactaa caaaccacag tatttccact ttaaatatag aactggtaaa
     2761 cagcactatc cttaaactaa aatcggtgac tcggtcatca agaaggtttt tgcccgcccg
     2821 tggatcttct tcagttatcc tagagaaaaa gaaagaggat gtcttgactg cactggaaat
     2881 cctctgtgaa aatgagtgta cagagacaga catcgagaaa gacaaatcta aattcctgga
     2941 gtttaagaaa tcaaaagaag aacactttta tcgaggtggc aaagtatcct ggtggaactt
     3001 ctatttttct tctgaaaact attcttcaga ttttgttaaa agggacagtt atgaaaagct
     3061 taaagattta atacactgct gggcagagtc tcctaaacca atatttgcaa aaatcatcaa
     3121 tctttatcat catccaggct gtggaggtac cacactggct atgcatgttc tctgggactt
     3181 aaagaaaaac ttcagatgtg ctgtgttaaa aaacaagaca actgattttg cagaaattgc
     3241 agagcaagtg atcaatctgg tcacctatag ggcaaagagc catcaggatt acattcctgt
     3301 gcttctcctt gtggatgatt ttgaagaaca agaaaatgtc tactttctac aaaatgccat
     3361 ccattccgtt ttagcagaaa aggatttgcg atatgaaaaa acattggtaa ttatcttaaa
     3421 ctgcatgaga tcccggaatc cagatgaaag tgcaaaattg gcagacagta ttgcactaaa
     3481 ttaccaactt tcttccaagg aacaaagagc ttttggtgcc aaactgaagg aaattgaaaa
     3541 gcagcacaag aactgtgaaa acttttattc cttcatgatc atgaaaagca attttgatga
     3601 aacatatata gaaaatgtag tcaggaatat cctaaaagga caggatgttg acagcaagga
     3661 agcacaactc atttccttcc tggctttact cagctcttat gttactgact ctacaatttc
     3721 agtttcacag tgtgaaatat ttttgggaat catatacact agtacaccct gggaacctga
     3781 aagcttagaa gacaagatgg gaacttattc tacacttcta ataaaaacag aagttgcaga
     3841 atatgggaga tacacaggtg tgcgtatcat tcaccctctg attgccctgt actgtctaaa
     3901 agaactggaa agaagctatc acttggataa atgtcaaatt gcattgaata tattagaaga
     3961 gaatttattc tatgattctg gaataggaag agacaaattt caacatgatg ttcaaactct
     4021 tctgcttaca agacagcgca aggtgtatgg agatgaaaca gacactctgt tttccccatt
     4081 aatggaagct ttacagaata aagacattga aaaggtcttg agtgcaggaa gtagacgatt
     4141 cccacaaaat gcattcattt gtcaagcctt agcaagacat ttctacatta aagagaagga
     4201 ctttaacaca gctctggact gggcacgtca ggccaaaatg aaagcaccta aaaattccta
     4261 tatttcagat acactaggtc aagtctacaa aagtgaaatc aaatggtggt tggatgggaa
     4321 caaaaactgt aggagcatta ctgttaatga cctaacacat ctcctagaag ctgcggaaaa
     4381 agcctcaaga gctttcaaag aatcccaaag gcaaactgat agtaaaaact atgaaaccga
     4441 gaactggtca ccacagaagt cccagagacg atatgacatg tataacacag cttgtttctt
     4501 gggtgaaata gaagttggtc tttacactat ccagattctt cagctcactc cctttttcca
     4561 caaagaaaat gaattatcca aaaaacatat ggtgcaattt ttatcaggaa agtggaccat
     4621 tcctcctgat cccagaaatg aatgttattt ggctcttagc aagttcacat cccacctaaa
     4681 aaatttacaa tcagatctga aaaggtgctt tgactttttt attgattata tggttcttct
     4741 gaaaatgagg tatacccaaa aagaaattgc agaaatcatg ttaagcaaga aagtcagtcg
     4801 ttgtttcagg aaatacacag aacttttctg tcatttggat ccatgtctat tacaaagtaa
     4861 agagagtcaa ttactccagg aggagaattg caggaaaaag ctagaagctc tgagagcaga
     4921 taggtttgct ggactcttgg aatatcttaa tccaaactac aaagatgcta ccaccatgga
     4981 aagtatagtg aatgaatatg ccttcctact gcagcaaaac tcaaaaaagc ccatgacaaa
     5041 tgagaaacaa aattccattt tggccaacat tattctgagt tgtctaaagc ccaactccaa
     5101 gttaattcaa ccacttacca cgctaaaaaa acaactccga gaggtcttgc aatttgtagg
     5161 actaagtcat caatatccag gtccttattt cttggcctgc ctcctgttct ggccagaaaa
     5221 tcaagagcta gatcaagatt ccaaactaat agaaaagtat gtttcatcct taaatagatc
     5281 cttcagggga cagtacaagc gcatgtgcag gtccaagcag gcaagcacac ttttctatct
     5341 gggcaaaagg aagggtctaa acagtattgt tcacaaggcc aaaatagagc agtactttga
     5401 taaagcacaa aatacaaatt ccctctggca cagtggggat gtgtggaaaa aaaatgaagt
     5461 caaagacctc ctgcgtcgtc taactggtca ggctgaaggc aagctaatct ctgtagaata
     5521 tggaacagag gaaaaaataa aaataccagt aatatctgtt tattcaggtc cactcagaag
     5581 tggtaggaac atagaaagag tgtctttcta cctaggattt tccattgaag gccctctggc
     5641 atatgatata gaagtaattc tcgagtaccc atacgacgta cctgattatg caacttatcc
     5701 ttatgatgta cctgattatg ctagctatcc ttatgatgtc ccagactacg catgagggcc
     5761 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     5821 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     5881 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     5941 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     6001 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     6061 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     6121 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     6181 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     6241 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     6301 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     6361 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     6421 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     6481 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     6541 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     6601 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     6661 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     6721 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     6781 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     6841 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
     6901 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
     6961 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
     7021 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
     7081 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
     7141 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
     7201 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
     7261 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
     7321 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
     7381 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     7441 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     7501 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     7561 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     7621 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     7681 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     7741 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     7801 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     7861 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7921 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7981 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     8041 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     8101 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     8161 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     8221 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     8281 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     8341 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     8401 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     8461 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     8521 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     8581 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     8641 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     8701 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     8761 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     8821 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8881 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8941 gatccggcaa acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc
     9001 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9061 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9121 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9181 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9241 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9301 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9361 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9421 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9481 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9541 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9601 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     9661 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     9721 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     9781 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     9841 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     9901 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     9961 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10021 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    10081 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    10141 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    10201 atctcccgat cccctatggt
//
