LOCUS V043538 5974 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V043538 VERSION V043538 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..1410 /label="RPL21(NM_000982)" /note="RPL21(NM_000982)" /gene="RPL21" CDS 1417..1506 /codon_start=1 /product="3 tandem Myc epitope tags" /transl_table=1 /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL" /label="3xMyc" polyA_signal 1541..1765 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 1811..2239 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2253..2582 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 2649..3443 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 3617..3738 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(3787..3803) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 3811..3827 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(3835..3865) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 3880..3901 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4189..4774) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(4945..5805) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(5806..5910) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgacgaaca caaagggaaa gaggagaggc 961 acccgatata tgttctctag gccttttaga aaacatggag ttgttccttt ggccacatat 1021 atgcgaatct ataagaaagg tgatattgta gacatcaagg gaatgggtac tgttcaaaaa 1081 ggaatgcccc acaagtgtta ccatggcaaa actggaagag tctacaatgt tacccagcat 1141 gctgttggca ttgttgtaaa caaacaagtt aagggcaaga ttcttgccaa gagaattaat 1201 gtgcgtattg agcacattaa gcactctaag agccgagata gcttcctgaa acgtgtgaag 1261 gaaaatgatc agaaaaagaa agaagccaaa gagaaaggta cctgggttca actaaagcgc 1321 cagcctgctc cacccagaga agcacacttt gtgagaacca atgggaagga gcctgagctg 1381 ctggaaccta ttccctatga attcatggca ctcgaggaac aaaaactaat atcagaggaa 1441 gatttggaac agaaactaat cagtgaagaa gatttagagc aaaagttaat ttccgaggag 1501 gacttatgag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag 1561 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact 1621 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt 1681 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat 1741 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg 1801 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 1861 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 1921 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 1981 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca 2041 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 2101 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 2161 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 2221 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc 2281 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 2341 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2401 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 2461 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 2521 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 2581 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat 2641 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 2701 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 2761 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 2821 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 2881 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 2941 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 3001 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 3061 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 3121 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 3181 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 3241 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 3301 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 3361 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 3421 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 3481 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3541 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 3601 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3721 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 3781 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3841 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 3901 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3961 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 4021 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 4081 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 4141 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4201 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 4261 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4321 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4381 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 4441 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4501 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4561 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4621 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4681 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggttttt ttgtttgcaa 4741 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4801 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4861 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4921 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4981 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 5041 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 5101 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 5161 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 5221 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 5281 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 5341 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 5401 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 5461 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 5521 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 5581 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 5641 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 5701 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 5761 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 5821 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5881 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 5941 cgacggatcg ggagatctcc cgatccccta tggt //