LOCUS V039906 7785 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V039906 VERSION V039906 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 955..3303 /label="RNF43(NM_017763)" /note="RNF43(NM_017763)" /gene="RNF43" CDS 3325..3351 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 3380..3604 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3650..4078 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4092..4421 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4488..5282 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5458..5579 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5628..5644) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5652..5668 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5676..5706) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5721..5742 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(6030..6618) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6789..7649) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7650..7754) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatgagt 961 ggtggccacc agctgcagct ggctgccctc tggccctggc tgctgatggc taccctgcag 1021 gcaggctttg gacgcacagg actggtactg gcagcagcgg tggagtctga aagatcagca 1081 gaacagaaag ctattatcag agtgatcccc ttgaaaatgg accccacagg aaaactgaat 1141 ctcactttgg aaggtgtgtt tgctggtgtt gctgaaataa ctccagcaga aggaaaatta 1201 atgcagtccc acccgctgta cctgtgcaat gccagtgatg acgacaatct ggagcctgga 1261 ttcatcagca tcgtcaagct ggagagtcct cgacgggccc cccgcccctg cctgtcactg 1321 gctagcaagg ctcggatggc gggtgagcga ggagccagtg ctgtcctctt tgacatcact 1381 gaggatcgag ctgctgctga gcagctgcag cagccgctgg ggctgacctg gccagtggtg 1441 ttgatctggg gtaatgacgc tgagaagctg atggagtttg tgtacaagaa ccaaaaggcc 1501 catgtgagga ttgagctgaa ggagcccccg gcctggccag attatgatgt gtggatccta 1561 atgacagtgg tgggcaccat ctttgtgatc atcctggctt cggtgctgcg catccggtgc 1621 cgcccccgcc acagcaggcc ggatccgctt cagcagagaa cagcctgggc catcagccag 1681 ctggccacca ggaggtacca ggccagctgc aggcaggccc ggggtgagtg gccagactca 1741 gggagcagct gcagctcagc ccctgtgtgt gccatctgtc tggaggagtt ctctgagggg 1801 caggagctac gggtcatttc ctgcctccat gagttccatc gtaactgtgt ggacccctgg 1861 ttacatcagc atcggacttg ccccctctgc atgttcaaca tcacagaggg agattcattt 1921 tcccagtccc tgggaccctc tcgatcttac caagaaccag gtcgaagact ccacctcatt 1981 cgccagcatc ccggccatgc ccactaccac ctccctgctg cctacctgtt gggcccttcc 2041 cggagtgcag tggctcggcc cccacgacct ggtcccttcc tgccatccca ggagccaggc 2101 atgggccctc ggcatcaccg cttccccaga gctgcacatc cccgggctcc aggagagcag 2161 cagcgcctgg caggagccca gcacccctat gcacaaggct ggggactgag ccacctccaa 2221 tccacctcac agcaccctgc tgcttgccca gtgcccctac gccgggccag gccccctgac 2281 agcagtggat ctggagaaag ctattgcaca gaacgcagtg ggtacctggc agatgggcca 2341 gccagtgact ccagctcagg gccctgtcat ggctcttcca gtgactctgt ggtcaactgc 2401 acggacatca gcctacaggg ggtccatggc agcagttcta ctttctgcag ctccctaagc 2461 agtgactttg accccctagt gtactgcagc cctaaagggg atccccagcg agtggacatg 2521 cagcctagtg tgacctctcg gcctcgttcc ttggactcgg tggtgcccac aggggaaacc 2581 caggtttcca gccatgtcca ctaccaccgc caccggcacc accactacaa aaagcggttc 2641 cagtggcatg gcaggaagcc tggcccagaa accggagtcc cccagtccag gcctcctatt 2701 cctcggacac agccccagcc agagccacct tctcctgatc agcaagtcac cagatccaac 2761 tcagcagccc cttcggggcg gctctctaac ccacagtgcc ccagggccct ccctgagcca 2821 gcccctggcc cagttgacgc ctccagcatc tgccccagta ccagcagtct gttcaacttg 2881 caaaaatcca gcctctctgc ccgacaccca cagaggaaaa ggcggggggg tccctccgag 2941 cccacccctg gctctcggcc ccaggatgca actgtgcacc cagcttgcca gatttttccc 3001 cattacaccc ccagtgtggc atatccttgg tccccagagg cacacccctt gatctgtgga 3061 cctccaggcc tggacaagag gctgctacca gaaaccccag gcccctgtta ctcaaattca 3121 cagccagtgt ggttgtgcct gactcctcgc cagcccctgg aaccacatcc acctggggag 3181 gggccttctg aatggagttc tgacaccgca gagggcaggc catgccctta tccgcactgc 3241 caggtgctgt cggcccagcc tggctcagag gaggaactcg aggagctgtg tgaacaggct 3301 gtgctcgagt ctagagggcc cttctaccca tacgatgttc cagattacgc ttgagtttaa 3361 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc 3421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag 3481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag 3541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct 3601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt 3661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 3721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 3781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 3841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 3901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 3961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 4021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa 4081 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 4141 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 4201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 4261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 4321 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga 4381 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta 4441 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag 4501 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 4561 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 4621 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 4681 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 4741 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 4801 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 4861 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 4921 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 4981 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 5041 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 5101 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 5161 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 5221 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 5281 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga 5341 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 5401 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac 5461 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 5521 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat 5581 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt 5641 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 5701 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 5761 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 5821 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 5881 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 5941 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6001 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 6061 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6121 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6181 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 6241 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6301 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6361 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6421 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 6481 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6541 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 6601 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6661 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6721 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6781 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 6841 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 6901 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 6961 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7021 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7081 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 7141 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7201 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7261 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7321 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7381 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7441 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7501 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7561 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7621 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7741 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc //