LOCUS       V039906                 7785 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V039906
VERSION     V039906
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             955..3303
                     /label="RNF43(NM_017763)"
                     /note="RNF43(NM_017763)"
                     /gene="RNF43"
     CDS             3325..3351
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    3380..3604
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3650..4078
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4092..4421
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4488..5282
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5458..5579
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5628..5644)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5652..5668
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5676..5706)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5721..5742
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6030..6618)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6789..7649)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7650..7754)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
      901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattcgc caccatgagt
      961 ggtggccacc agctgcagct ggctgccctc tggccctggc tgctgatggc taccctgcag
     1021 gcaggctttg gacgcacagg actggtactg gcagcagcgg tggagtctga aagatcagca
     1081 gaacagaaag ctattatcag agtgatcccc ttgaaaatgg accccacagg aaaactgaat
     1141 ctcactttgg aaggtgtgtt tgctggtgtt gctgaaataa ctccagcaga aggaaaatta
     1201 atgcagtccc acccgctgta cctgtgcaat gccagtgatg acgacaatct ggagcctgga
     1261 ttcatcagca tcgtcaagct ggagagtcct cgacgggccc cccgcccctg cctgtcactg
     1321 gctagcaagg ctcggatggc gggtgagcga ggagccagtg ctgtcctctt tgacatcact
     1381 gaggatcgag ctgctgctga gcagctgcag cagccgctgg ggctgacctg gccagtggtg
     1441 ttgatctggg gtaatgacgc tgagaagctg atggagtttg tgtacaagaa ccaaaaggcc
     1501 catgtgagga ttgagctgaa ggagcccccg gcctggccag attatgatgt gtggatccta
     1561 atgacagtgg tgggcaccat ctttgtgatc atcctggctt cggtgctgcg catccggtgc
     1621 cgcccccgcc acagcaggcc ggatccgctt cagcagagaa cagcctgggc catcagccag
     1681 ctggccacca ggaggtacca ggccagctgc aggcaggccc ggggtgagtg gccagactca
     1741 gggagcagct gcagctcagc ccctgtgtgt gccatctgtc tggaggagtt ctctgagggg
     1801 caggagctac gggtcatttc ctgcctccat gagttccatc gtaactgtgt ggacccctgg
     1861 ttacatcagc atcggacttg ccccctctgc atgttcaaca tcacagaggg agattcattt
     1921 tcccagtccc tgggaccctc tcgatcttac caagaaccag gtcgaagact ccacctcatt
     1981 cgccagcatc ccggccatgc ccactaccac ctccctgctg cctacctgtt gggcccttcc
     2041 cggagtgcag tggctcggcc cccacgacct ggtcccttcc tgccatccca ggagccaggc
     2101 atgggccctc ggcatcaccg cttccccaga gctgcacatc cccgggctcc aggagagcag
     2161 cagcgcctgg caggagccca gcacccctat gcacaaggct ggggactgag ccacctccaa
     2221 tccacctcac agcaccctgc tgcttgccca gtgcccctac gccgggccag gccccctgac
     2281 agcagtggat ctggagaaag ctattgcaca gaacgcagtg ggtacctggc agatgggcca
     2341 gccagtgact ccagctcagg gccctgtcat ggctcttcca gtgactctgt ggtcaactgc
     2401 acggacatca gcctacaggg ggtccatggc agcagttcta ctttctgcag ctccctaagc
     2461 agtgactttg accccctagt gtactgcagc cctaaagggg atccccagcg agtggacatg
     2521 cagcctagtg tgacctctcg gcctcgttcc ttggactcgg tggtgcccac aggggaaacc
     2581 caggtttcca gccatgtcca ctaccaccgc caccggcacc accactacaa aaagcggttc
     2641 cagtggcatg gcaggaagcc tggcccagaa accggagtcc cccagtccag gcctcctatt
     2701 cctcggacac agccccagcc agagccacct tctcctgatc agcaagtcac cagatccaac
     2761 tcagcagccc cttcggggcg gctctctaac ccacagtgcc ccagggccct ccctgagcca
     2821 gcccctggcc cagttgacgc ctccagcatc tgccccagta ccagcagtct gttcaacttg
     2881 caaaaatcca gcctctctgc ccgacaccca cagaggaaaa ggcggggggg tccctccgag
     2941 cccacccctg gctctcggcc ccaggatgca actgtgcacc cagcttgcca gatttttccc
     3001 cattacaccc ccagtgtggc atatccttgg tccccagagg cacacccctt gatctgtgga
     3061 cctccaggcc tggacaagag gctgctacca gaaaccccag gcccctgtta ctcaaattca
     3121 cagccagtgt ggttgtgcct gactcctcgc cagcccctgg aaccacatcc acctggggag
     3181 gggccttctg aatggagttc tgacaccgca gagggcaggc catgccctta tccgcactgc
     3241 caggtgctgt cggcccagcc tggctcagag gaggaactcg aggagctgtg tgaacaggct
     3301 gtgctcgagt ctagagggcc cttctaccca tacgatgttc cagattacgc ttgagtttaa
     3361 acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3601 atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt
     3661 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     3721 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     3781 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     3841 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     3901 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     3961 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4021 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa
     4081 ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4141 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     4201 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     4261 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     4321 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
     4381 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta
     4441 tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     4501 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     4561 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     4621 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
     4681 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     4741 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     4801 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     4861 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     4921 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     4981 tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg
     5041 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     5101 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     5161 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     5221 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     5281 gagcgggact ctggggttcg cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     5341 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     5401 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     5461 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5521 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5581 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     5641 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     5701 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     5761 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     5821 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     5881 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     5941 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6001 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6061 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6121 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6181 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6241 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6301 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6361 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6421 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6481 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6541 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6601 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6661 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6721 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6781 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6841 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     6901 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     6961 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7021 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7081 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7141 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7201 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7261 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7321 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7381 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7441 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7501 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7561 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7621 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     7681 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     7741 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
