LOCUS       V025629                 8837 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V025629
VERSION     V025629
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        235..614
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        615..818
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        863..881
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             923..1576
                     /codon_start=1
                     /gene=""
                     /product="glutathione S-transferase from Schistosoma
                     japonicum"
                     /transl_table=1
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
                     /label="GST"
     CDS             1592..4387
                     /label="RBM12(NM_006047)"
                     /note="RBM12(NM_006047)"
                     /gene="RBM12"
     polyA_signal    4434..4658
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4704..5132
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5146..5475
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5542..6336
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6510..6631
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6680..6696)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6704..6720
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6728..6758)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6773..6794
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7082..7670)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7841..8701)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8702..8806)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatgtcccc tatactaggt tattggaaaa ttaagggcct
      961 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
     1021 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
     1081 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
     1141 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
     1201 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
     1261 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
     1321 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
     1381 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
     1441 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
     1501 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
     1561 cgaccatcct ccaaaatcgg atctgggatc catggctgtg gtcatccgtt tgcaaggtct
     1621 cccaattgtg gcggggacca tggacattcg ccacttcttc tctggattga ccattcctga
     1681 tgggggcgtg catattgtag ggggtgaact gggtgaggct ttcatcgttt ttgccactga
     1741 tgaagatgca aggcttggta tgatgcgcac aggtggtaca attaaagggt caaaagtaac
     1801 actattgttg agtagtaaga cggaaatgca gaatatgatt gaactgagtc gtaggcgttt
     1861 tgaaactgcc aacttagata taccaccagc aaatgccagt agatcaggac caccacctag
     1921 ctcaggaatg agtagcaggg taaacttgcc cacaacagta tccaacttta ataatccatc
     1981 acccagtgta gttactgcca ccacttctgt tcatgaaagc aacaaaaaca tacagacatt
     2041 ttccacagcc agcgtaggaa cagctcctcc aaatatgggg gcttcctttg ggagcccaac
     2101 gtttagctca actgttccaa gcacagcctc tccaatgaac acagtcccgc cgccaccaat
     2161 tcctccaatt ccagcgatgc catctctgcc accaatgcca tccattcccc caattccagt
     2221 tcctcctcca gtacctacat tgcctcctgt gcctcctgtg cccccgattc ccccagttcc
     2281 ttctgtgcca cccatgaccc cactgccacc catgtcgggc atgccgccct tgaatccgcc
     2341 acctgtggca cctctacctg ctggaatgaa tggctctgga gcacctatga atttgaacaa
     2401 taatctgaat cctatgtttc ttggtccgtt gaatcctgtt aaccctatcc agatgaactc
     2461 tcagagcagt gtgaagccac tccccatcaa ccctgatgat ctgtatgtca gtgtgcatgg
     2521 aatgcccttt tctgcaatgg aaaatgatgt cagagatttt tttcatgggc tccgtgttga
     2581 tgcagtgcat ttgttgaaag atcatgtagg tcgaaataat gggaatggat tggttaagtt
     2641 tctctcccct caagatacat ttgaagcttt gaaacgaaac agaatgctga tgattcaacg
     2701 ctatgtggaa gttagccctg ccacagaaag acagtgggta gctgctggag gccatatcac
     2761 ttttaagcaa aatatgggac cttctggaca aactcatccc cctcctcaga cacttcccag
     2821 gtcaaaatcg cccagtgggc agaaaagatc aaggtcaaga tcaccacatg aggctggttt
     2881 ttgtgtttac ttgaaagggc taccatttga agcagaaaac aaacatgtca ttgatttttt
     2941 taaaaagctg gatattgtgg aagatagtat ttatatagct tatggaccca atgggaaagc
     3001 aactggcgaa ggctttgtag agttcagaaa tgaggctgac tataaggctg ctctgtgtcg
     3061 tcataaacag tacatgggca atcgctttat tcaagttcat ccaattacta agaaaggtat
     3121 gctagaaaag atagatatga ttcgaaaaag actgcagaac ttcagctatg accagaggga
     3181 aatgatacta aatccagagg gggatgtcaa ctctgccaaa gtctgtgccc acataacaaa
     3241 tattccattc agcattacaa agatggatgt tcttcagttc ctagaaggaa tcccagtgga
     3301 tgaaaatgct gtacatgttc ttgttgataa caatgggcaa ggtctaggac aggcattggt
     3361 tcagtttaaa aatgaagatg atgcacgtaa gtctgaacgc ttacaccgta aaaaacttaa
     3421 tgggagagaa gcttttgttc atgtagttac cctagaagat atgagagaga ttgagaaaaa
     3481 tccccctgcc caaggaaaaa agggattaaa gatgcctgtg ccaggtaatc ctgcagttcc
     3541 aggaatgccc aatgcgggac tgcccggtgt gggactgccc agtgcaggac ttcccggtgc
     3601 aggcctgccc agcacaggac tgcctggttc agcaataacc agtgcaggac tgcctggtgc
     3661 gggaatgccc agtgcaggaa tacctagtgc aggaggtgaa gagcatgcct tcctgactgt
     3721 aggatcaaag gaagccaata atgggcctcc atttaacttt cctggtaatt ttggtggatc
     3781 aaatgccttt gggccaccaa tccctcctcc aggattagga ggcggggcct ttggtgatgc
     3841 taggcctggt atgccttcag ttggaaacag tggtttgcct ggtctaggac tggatgttcc
     3901 gggttttgga ggtggaccaa acaatttaag tgggccatcg ggatttggag ggggccctca
     3961 gaattttgga aatggccctg gtagcttagg cggtcccccg gggtttggaa gtggccctcc
     4021 tggtcttgga agtgcccctg ggcatttggg tgggccacca gcttttgggc ctggccccgg
     4081 ccccggcccc ggccctggcc caatccatat tggtggtccc cctggctttg catctagttc
     4141 tggaaaacca ggaccgacag taattaaagt gcaaaacatg ccctttactg tgtctattga
     4201 tgagatttta gatttctttt atggctatca agtaatccca ggctcagtgt gtttaaaata
     4261 caatgaaaaa ggtatgccca caggtgaagc catggtggcc tttgagtctc gggatgaagc
     4321 cacagctgct gtcattgact taaatgacag gcctataggt tcaagaaaag taaaacttgt
     4381 attagggtag ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     4441 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     4501 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     4561 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     4621 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     4681 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     4741 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     4801 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     4861 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     4921 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     4981 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5041 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5101 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     5161 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5221 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     5281 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     5341 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     5401 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     5461 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     5521 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     5581 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     5641 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     5701 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     5761 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     5821 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     5881 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     5941 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     6001 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     6061 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     6121 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     6181 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     6241 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     6301 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     6361 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     6421 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     6481 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     6541 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     6601 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     6661 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8821 aagtgccacc tgacgtc
//
