LOCUS       V038705                 9329 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V038705
VERSION     V038705
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             874..1590
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /transl_table=1
                     /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL
                     TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG
                     IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV
                     LLEFVTAAGITLGMDELYK"
                     /label="EGFP"
     CDS             1660..4701
                     /label="Parp1(NM_007415)"
                     /note="Parp1(NM_007415)"
                     /gene="Parp1"
     CDS             4708..4734
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4738..4764
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4768..4794
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4896..5120
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      5166..5594
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5608..5937
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             6004..6798
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6972..7093
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(7142..7158)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    7166..7182
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(7190..7220)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    7235..7256
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(7544..8129)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(8300..9160)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(9161..9265)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatggtga gcaagggcga ggagctgttc
      901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     1561 atcactctcg gcatggacga gctgtacaag tccggactca gatctcgagg cggcggaggg
     1621 agtggaggag ggggaagcgg aggcggaggg agtaagctta tggcggaggc ctcggagagg
     1681 ctttatcgag tggagtacgc gaagagcggg cgcgcctctt gcaagaaatg cagcgagagt
     1741 attcccaagg actccctccg catggccatc atggtgcagt cacccatgtt cgatgggaaa
     1801 gtcccacact ggtaccactt ctcctgcttc tggaaggtgg gccactccat ccggcagcct
     1861 gatgttgagg tggatggctt ctctgagctg cgctgggatg atcagcagaa ggtcaagaag
     1921 acggccgagg ctggaggcgt ggcaggcaaa ggccaggatg gaagtggcgg caaggcggag
     1981 aagacattgg gtgacttttt agcggagtac gccaagtcca acaggagcat gtgcaagggc
     2041 tgcctggaga agatagagaa gggccagatg cgcctgtcca agaagatggt ggatccagag
     2101 aagccacagc tgggtatgat tgaccgctgg taccatccaa cttgctttgt caagaagcgg
     2161 gacgagctgg gcttccggcc tgagtacagt gccagtcagc tcaagggctt tagcctcctc
     2221 tctgcagaag acaaagaagc tctgaagaag cagctcccgg ccatcaagaa tgaaggaaag
     2281 agaaaaggtg acgaggtgga tggaacagat gaagtggcca aaaagaaatc taagaaaggg
     2341 aaggacaagg atagtagtaa gctggagaag gccctcaagg ctcagaatga gctgatctgg
     2401 aatatcaaag acgagctgaa gaaagcgtgt tccaccaacg acctgaagga gctgctcatc
     2461 ttcaaccagc agcaggtgcc gtcaggagag tcagcgatct tggacagagt tgctgacggc
     2521 atggcgtttg gggcccttct gccctgcaag gagtgttcag gccagctggt ctttaagagc
     2581 gacgcttatt actgtactgg ggatgtcact gcctggacca agtgcatggt caagacacag
     2641 aatcctagcc gaaaggaatg ggtaactcca aaggaattcc gagaaatatc ctacctcaag
     2701 aagttaaagg tcaaaaaaca ggaccgaata ttccctccag aaagcagcgc cccagcacca
     2761 ctggcactgc ccctctctgt cacctcagca cccacagctg tgaactcctc tgctccagca
     2821 gacaagcccc tgtctaacat gaagatcctg actcttggga agctctccca gaacaaggac
     2881 gaagcaaaag ctgtgattga gaaactcgga ggcaagttga caggatctgc caacaaggcc
     2941 tccttgtgta tcagcactaa aaaggaggtg gagaagatga gtaagaagat ggaggaagtg
     3001 aaagcggcca acgttcgagt tgtgtgtgag gacttcctcc aggacgtgtc tgcctccact
     3061 aaaagcctcc aagagctgct ctcggcccac agcttgtcct cgtggggggc tgaggtgaag
     3121 gcagagcctg gtgaagtggt ggcccccaag gggaagtcag ctgcaccctc caagaagagc
     3181 aagggtgctg tcaaggagga aggtgtcaac aaatctgaaa agaggatgaa attaactctg
     3241 aagggaggag cagccgttga tcctgactct ggtctggaac actctgcaca cgtcctggag
     3301 aaaggtggga aggtgttcag cgccacactt ggcctggtgg acattgtgaa agggacgaac
     3361 tcctattaca aactgcagct tctggaggac gacaaggaga gcaggtactg gatcttccgg
     3421 tcctggggcc gggtgggcac agttatcggc agtaacaaac ttgagcagat gccctccaaa
     3481 gaggacgctg ttgagcactt catgaagctg tatgaagaga agactgggaa tgcctggcac
     3541 tcgaaaaact tcacaaagta tcccaagaag ttctaccctc tggagattga ctatggccag
     3601 gacgaagagg cagtaaagaa gctgacggtg aagcctggca ccaagtcgaa gctgccgaag
     3661 ccagtgcagg agctcgtggg gatgatcttc gacgtggaga gcatgaaaaa ggccttggtg
     3721 gagtacgaga ttgacctcca gaagatgccc ttggggaagc tgagcagaag gcagatccag
     3781 gccgcctact ctatcctcag cgaggtccag caggcagtgt ctcaaggcag cagtgaatcc
     3841 cagatcctag atctctccaa tcgcttctac actctgatcc cccatgactt tggaatgaag
     3901 aagcccccac tcctgaacaa cgcagacagc gtgcaggcca aggtggagat gctagacaac
     3961 ctcctggaca tcgaggtggc ctatagtctt ctcaggggtg gctctgacga cagcagcaag
     4021 gatcccatcg acgtcaacta cgagaaactc aaaactgaca ttaaggtggt tgacagagat
     4081 tctgaagagg ccgaggtcat caggaagtac gtgaagaaca ctcatgctac cacgcacaac
     4141 gcctatgacc tggaagtgat cgatatcttc aagatagagc gcgaggggga gagccagcgc
     4201 tacaagccct tcaggcagct tcacaaccgg aggctgctgt ggcacggctc caggaccacc
     4261 aactttgctg gcatcctgtc gcagggtctg cggatagccc cacctgaagc gcctgtgaca
     4321 ggctacatgt ttgggaaagg gatctacttt gccgacatgg tgtccaaaag tgcaaactac
     4381 tgccacacat ctcagggaga cccgattggc ttaatactgc tgggagaggt tgcccttgga
     4441 aacatgtatg aactcaagca tgcttcacat atcagcaagt tacccaaggg caagcacagt
     4501 gtcaaaggtt tgggaaaaac cacccctgac ccttcggcca gcatcaccct ggagggtgta
     4561 gaggttccac tgggaacagg gatcccatct ggtgtcaacg acacctgcct gctgtataat
     4621 gagtacattg tctacgacat tgctcaggtg aatctcaaat acctgctgaa actcaagttc
     4681 aattttaaga catccctgtg gggtacctac ccatacgacg tacctgatta tgcaacttat
     4741 ccttatgatg tacctgatta tgctagctat ccttatgatg tcccagacta cgcataggat
     4801 ccactagtcc agtgtggtgg aattctgcag atatccagca cagtggcggc cgctcgagtc
     4861 tagagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     4921 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     4981 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5041 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5101 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     5161 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     5221 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     5281 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     5341 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     5401 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     5461 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     5521 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     5581 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     5641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     5701 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5761 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5821 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     5881 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     5941 ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg aggatcgttt
     6001 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta
     6061 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg
     6121 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa
     6181 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct
     6241 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg
     6301 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca
     6361 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat
     6421 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac
     6481 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc
     6541 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa
     6601 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag
     6661 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc
     6721 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt
     6781 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca
     6841 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     6901 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     6961 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     7081 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     7141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     7201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     7261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     7321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     7381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     7441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     7501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     7561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     7621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     7681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     7741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     7801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     7861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     7921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     7981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     8041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tttttttgtt tgcaagcagc
     8101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg
     9301 gatcgggaga tctcccgatc ccctatggt
//
