LOCUS       pcDNA3.1-NSF(human)     7619 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V036084
VERSION     V036084
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3139
                     /label="NSF(NM_006178)"
                     /note="NSF(NM_006178)"
                     /gene="NSF"
     polyA_signal    3186..3410
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3456..3884
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3898..4227
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4294..5088
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5262..5383
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5432..5448)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5456..5472
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5480..5510)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5525..5546
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5834..6419)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6590..7450)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7451..7555)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg gcgggccgga gcatgcaagc ggcaagatgt cctacagatg aattatcttt
      961 aaccaattgt gcagttgtga atgaaaagga tttccagtct ggccagcatg tgattgtgag
     1021 gacctctccc aatcacaggt acacatttac actgaagaca catccatcgg tggttccagg
     1081 gagcattgca ttcagtttac ctcagagaaa atgggctggg ctttctattg ggcaagaaat
     1141 agaagtctcc ttatatacat ttgacaaagc caaacagtgt attggcacaa tgaccatcga
     1201 gattgatttc ctgcagaaaa aaagcattga ctccaaccct tatgacaccg acaagatggc
     1261 agcagaattt attcagcaat tcaacaacca ggccttctca gtgggacaac agcttgtctt
     1321 tagcttcaat gaaaagcttt ttggcttact ggtgaaggac attgaagcca tggatcctag
     1381 catcctgaag ggagagcctg cgacagggaa aaggcagaag attgaagtag gactggttgt
     1441 tggaaacagt caagttgcat ttgaaaaagc agaaaattcg tcacttaatc ttattggcaa
     1501 agctaaaacc aaggaaaatc gccaatcaat tatcaatcct gactggaact ttgaaaaaat
     1561 gggaatagga ggtctagaca aggaattttc agatattttc cgacgagcat ttgcttcccg
     1621 agtatttcct ccagagattg tggagcagat gggttgtaaa catgttaaag gcatcctgtt
     1681 atatggaccc ccaggttgtg gtaagactct cttggctcga cagattggca agatgttgaa
     1741 tgcaagagag cccaaagtgg tcaatgggcc agaaatcctt aacaaatatg tgggagaatc
     1801 agaggctaac attcgcaaac tttttgctga tgctgaagag gagcaaagga ggcttggtgc
     1861 taacagtggt ttgcacatca tcatctttga tgaaattgat gccatctgca agcagagagg
     1921 gagcatggct ggtagcacgg gagttcatga cactgttgtc aaccagttgc tgtccaaaat
     1981 tgatggcgtg gagcagctaa acaacatcct agtcattgga atgaccaata gaccagatct
     2041 gatagatgag gctcttctta gacctggaag actggaagtt aaaatggaga taggcttgcc
     2101 agatgagaaa ggccgactac agattcttca catccacaca gcaagaatga gagggcatca
     2161 gttactctct gctgatgtag acattaaaga actggccgtg gagaccaaga atttcagtgg
     2221 tgctgaattg gagggtctgg tgcgagcagc ccagtccact gctatgaata gacacataaa
     2281 ggccagtact aaagtggaag tggacatgga gaaagcagaa agcctgcaag tgacgagagg
     2341 agacttcctt gcttctttgg agaatgatat caaaccagcc tttggcacaa accaagaaga
     2401 ttatgcaagt tacattatga acggtatcat caaatggggt gacccagtta ctcgagttct
     2461 agatgatggg gagctgctgg tgcagcagac taagaacagt gaccgcacac cattggtcag
     2521 cgtgcttctg gaaggccctc ctcacagtgg gaagactgct ttagctgcaa aaattgcaga
     2581 ggaatccaac ttcccgttca tcaagatctg ttctcctgat aaaatgattg gcttttctga
     2641 aacagccaaa tgtcaggcca tgaagaagat ctttgatgat gcgtacaaat cccagctcag
     2701 ttgtgtggtt gtggatgaca ttgagagatt gcttgattac gtccctattg gccctcgatt
     2761 ttcaaatctt gtattacagg ctcttctcgt tttactgaaa aaggcacctc ctcagggccg
     2821 caagcttctt atcattggga ccactagccg caaagatgtc cttcaggaga tggaaatgct
     2881 taacgctttc agcaccacca tccacgtgcc caacattgcc acaggagagc agctgttgga
     2941 agctttggag cttttgggca acttcaagga taaggaacgc accacaattg cacagcaagt
     3001 caaagggaag aaggtctgga taggaatcaa gaagttacta atgctgatcg agatgtccct
     3061 acagatggat cctgaatacc gtgtgagaaa attcttggcc ctcttaagag aagaaggagc
     3121 tagccccctt gattttgatt gactcgagtc tagagggccc gtttaaaccc gctgatcagc
     3181 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     3241 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     3301 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     3361 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     3421 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     3481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     3781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     3841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     3901 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3961 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4021 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4081 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4141 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     4201 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     4261 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     4321 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     4381 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     4441 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     4501 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     4561 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     4621 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     4681 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     4741 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     4801 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     4861 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     4921 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     4981 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5041 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5101 ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg
     5161 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5221 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5281 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5341 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5401 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5461 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5521 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5581 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5641 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5701 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5761 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5821 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5881 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5941 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     6001 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     6061 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6121 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6181 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6241 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6301 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6361 ctggtagcgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6421 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6481 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6541 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     7021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7561 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggt
//
