LOCUS       V018929                 7615 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V018929
VERSION     V018929
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             877..903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             910..3135
                     /label="NR3C1(NM_001020825)"
                     /note="NR3C1(NM_001020825)"
                     /gene="NR3C1"
     polyA_signal    3182..3406
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      3452..3880
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        3894..4223
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             4290..5084
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    5258..5379
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(5428..5444)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    5452..5468
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5476..5506)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    5521..5542
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(5830..6415)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(6586..7446)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(7447..7551)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgcc accatgtacc cttatgacgt ccccgattac
      901 gccggtacca tggactccaa agaatcatta actcctggta gagaagaaaa ccccagcagt
      961 gtgcttgctc aggagagggg agatgtgatg gacttctata aaaccctaag aggaggagct
     1021 actgtgaagg tttctgcgtc ttcaccctca ctggctgtcg cttctcaatc agactccaag
     1081 cagcgaagac ttttggttga ttttccaaaa ggctcagtaa gcaatgcgca gcagccagat
     1141 ctgtccaaag cagtttcact ctcaatggga ctgtatatgg gagagacaga aacaaaagtg
     1201 atgggaaatg acctgggatt cccacagcag ggccaaatca gcctttcctc gggggaaaca
     1261 gacttaaagc ttttggaaga aagcattgca aacctcaata ggtcgaccag tgttccagag
     1321 aaccccaaga gttcagcatc cactgctgtg tctgctgccc ccacagagaa ggagtttcca
     1381 aaaactcact ctgatgtatc ttcagaacag caacatttga agggccagac tggcaccaac
     1441 ggtggcaatg tgaaattgta taccacagac caaagcacct ttgacatttt gcaggatttg
     1501 gagttttctt ctgggtcccc aggtaaagag acgaatgaga gtccttggag atcagacctg
     1561 ttgatagatg aaaactgttt gctttctcct ctggcgggag aagacgattc attccttttg
     1621 gaaggaaact cgaatgagga ctgcaagcct ctcattttac cggacactaa acccaaaatt
     1681 aaggataatg gagatctggt tttgtcaagc cccagtaatg taacactgcc ccaagtgaaa
     1741 acagaaaaag aagatttcat cgaactctgc acccctgggg taattaagca agagaaactg
     1801 ggcacagttt actgtcaggc aagctttcct ggagcaaata taattggtaa taaaatgtct
     1861 gccatttctg ttcatggtgt gagtacctct ggaggacaga tgtaccacta tgacatgaat
     1921 acagcatccc tttctcaaca gcaggatcag aagcctattt ttaatgtcat tccaccaatt
     1981 cccgttggtt ccgaaaattg gaataggtgc caaggatctg gagatgacaa cttgacttct
     2041 ctggggactc tgaacttccc tggtcgaaca gttttttcta atggctattc aagccccagc
     2101 atgagaccag atgtaagctc tcctccatcc agctcctcaa cagcaacaac aggaccacct
     2161 cccaaactct gcctggtgtg ctctgatgaa gcttcaggat gtcattatgg agtcttaact
     2221 tgtggaagct gtaaagtttt cttcaaaaga gcagtggaag gacagcacaa ttacctatgt
     2281 gctggaagga atgattgcat catcgataaa attcgaagaa aaaactgccc agcatgccgc
     2341 tatcgaaaat gtcttcaggc tggaatgaac ctggaagctc gaaaaacaaa gaaaaaaata
     2401 aaaggaattc agcaggccac tacaggagtc tcacaagaaa cctctgaaaa tcctggtaac
     2461 aaaacaatag ttcctgcaac gttaccacaa ctcaccccta ccctggtgtc actgttggag
     2521 gttattgaac ctgaagtgtt atatgcagga tatgatagct ctgttccaga ctcaacttgg
     2581 aggatcatga ctacgctcaa catgttagga gggcggcaag tgattgcagc agtgaaatgg
     2641 gcaaaggcaa taccaggttt caggaactta cacctggatg accaaatgac cctactgcag
     2701 tactcctgga tgtttcttat ggcatttgct ctggggtgga gatcatatag acaatcaagt
     2761 gcaaacctgc tgtgttttgc tcctgatctg attattaatg agcagagaat gactctaccc
     2821 tgcatgtacg accaatgtaa acacatgctg tatgtttcct ctgagttaca caggcttcag
     2881 gtatcttatg aagagtatct ctgtatgaaa accttactgc ttctctcttc agttcctaag
     2941 gacggtctga agagccaaga gctatttgat gaaattagaa tgacctacat caaagagcta
     3001 ggaaaagcca ttgtcaagag ggaaggaaac tccagccaga actggcagcg gttttatcaa
     3061 ctgacaaaac tcttggattc tatgcatgaa aatgttatgt ggttaaaacc agaaagcaca
     3121 tctcacacat taatctgact cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     3181 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3241 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3301 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3361 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3421 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     3481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     3541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     3601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     3661 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     3721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     3781 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     3841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     3901 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3961 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     4021 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     4081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     4141 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     4201 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     4261 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     4321 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     4381 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     4441 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     4501 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     4561 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     4621 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     4681 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     4741 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     4801 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     4861 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     4921 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     4981 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     5041 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     5101 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     5161 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     5221 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     5281 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5341 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     5401 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     5461 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5521 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5581 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5641 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     6001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     6061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     6121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     6181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     6241 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     6301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     6361 tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     6421 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     6481 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6541 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     6601 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     6661 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     6721 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     6781 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     6841 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     6901 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     6961 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     7021 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     7081 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     7141 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     7201 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     7261 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     7321 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     7381 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     7441 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7501 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7561 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggt
//
