LOCUS       V020580                 8495 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V020580
VERSION     V020580
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             908..3934
                     /label="NLRP7(NM_139176)"
                     /note="NLRP7(NM_139176)"
                     /gene="NLRP7"
     CDS             3941..3967
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             3971..3997
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             4001..4027
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     polyA_signal    4062..4286
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4332..4760
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        4774..5103
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5170..5964
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6138..6259
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6308..6324)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6332..6348
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6356..6386)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6401..6422
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6710..7295)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7466..8326)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8327..8431)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cgccaccatg acatcgcccc agctagagtg gactctgcag acccttctgg agcagctgaa
      961 cgaggatgaa ttaaagagtt tcaaatccct tttatgggct tttcccctcg aagacgtgct
     1021 acagaagacc ccatggtctg aggtggaaga ggctgatggc aagaaactgg cagaaattct
     1081 ggtcaacacc tcctcagaaa attggataag gaatgcgact gtgaacatct tggaagagat
     1141 gaatctcacg gaattgtgta agatggcaaa ggctgagatg atggaggacg gacaggtgca
     1201 agaaatagat aatcctgagc tgggagatgc agaagaagac tcggagttag caaagccagg
     1261 tgaaaaggaa ggatggagaa attcaatgga gaaacagtct ttggtctgga agaacacctt
     1321 ttggcaagga gacattgaca atttccatga cgacgtcact ctgagaaacc aacggttcat
     1381 tccattcttg aatcccagaa cacccaggaa gctaacacct tacacggtgg tgctgcacgg
     1441 ccccgcaggc gtggggaaaa ccacgctggc caaaaagtgt atgctggact ggacagactg
     1501 caacctcagc ccgacgctca gatacgcgtt ctacctcagc tgcaaggagc tcagccgcat
     1561 gggcccctgc agttttgcag agctgatctc caaagactgg cctgaattgc aggatgacat
     1621 tccaagcatc ctagcccaag cacagagaat cctgttcgtg gtcgatggcc ttgatgagct
     1681 gaaagtccca cctggggcgc tgatccagga catctgcggg gactgggaga agaagaagcc
     1741 ggtgcccgtc ctcctgggga gtttgctgaa gaggaagatg ttacccaggg cagccttgct
     1801 ggtcaccacg cggcccaggg cactgaggga cctccagctc ctggcgcagc agccgatcta
     1861 cgtaagggtg gagggcttcc tggaggagga caggagggcc tatttcctga gacactttgg
     1921 agacgaggac caagccatgc gtgcctttga gctaatgagg agcaacgcgg ccctgttcca
     1981 gctgggctcg gcccccgcgg tgtgctggat tgtgtgcacg actctgaagc tgcagatgga
     2041 gaagggggag gacccggtcc ccacctgcct cacccgcacg gggctgttcc tgcgtttcct
     2101 ctgcagccgg ttcccgcagg gcgcacagct gcggggcgcg ctgcggacgc tgagcctcct
     2161 ggccgcgcag ggcctgtggg cgcagatgtc cgtgttccac cgagaggacc tggaaaggct
     2221 cggggtgcag gagtccgacc tccgtctgtt cctggacgga gacatcctcc gccaggacag
     2281 agtctccaaa ggctgctact ccttcatcca cctcagcttc cagcagtttc tcactgccct
     2341 gttctacgcc ctggagaagg aggaggggga ggacagggac ggccacgcct gggacatcgg
     2401 ggacgtacag aagctgcttt ccggagaaga aagactcaag aaccccgacc tgattcaagt
     2461 aggacacttc ttattcggcc tcgctaacga gaagagagcc aaggagttgg aggccacttt
     2521 tggctgccgg atgtcaccgg acatcaaaca ggaattgctg caatgcaaag cacatcttca
     2581 tgcaaataag cccttatccg tgaccgacct gaaggaggtc ttgggctgcc tgtatgagtc
     2641 tcaggaggag gagctggcga aggtggtggt ggccccgttc aaggaaattt ctattcacct
     2701 gacaaatact tctgaagtga tgcattgttc cttcagcctg aagcattgtc aagacttgca
     2761 gaaactctca ctgcaggtag caaagggggt gttcctggag aattacatgg attttgaact
     2821 ggacattgaa tttgaaagct caaacagcaa cctcaagttt ctggaagtga aacaaagctt
     2881 cctgagtgac tcttctgtgc ggattctttg tgaccacgta acccgtagca cctgtcatct
     2941 gcagaaagtg gagattaaaa acgtcacccc tgacaccgcg taccgggact tctgtcttgc
     3001 tttcattggg aagaagaccc tcacgcacct gaccctggca gggcacatcg agtgggaacg
     3061 cacgatgatg ctgatgctgt gtgacctgct cagaaatcat aaatgcaacc tgcagtacct
     3121 gaggttggga ggtcactgtg ccaccccgga gcagtgggct gaattcttct atgtcctcaa
     3181 agccaaccag tccctgaagc acctgcgtct ctcagccaat gtgctcctgg atgagggtgc
     3241 catgttgctg tacaagacca tgacacgccc aaaacacttc ctgcagatgt tgtcgttgga
     3301 aaactgtcgt cttacagaag ccagttgcaa ggaccttgct gctgtcttgg ttgtcagcaa
     3361 gaagctgaca cacctgtgct tggccaagaa ccccattggg gatacagggg tgaagtttct
     3421 gtgtgagggc ttgagttacc ctgattgtaa actgcagacc ttggtgttac agcaatgcag
     3481 cataaccaag cttggctgta gatatctctc agaggcgctc caagaagcct gcagcctcac
     3541 aaacctggac ttgagtatca accagatagc tcgtggattg tggattctct gtcaggcatt
     3601 agagaatcca aactgtaacc taaaacacct acgcctctgg agctgctccc tcatgccttt
     3661 ctattgtcag catcttggat ctgctctcct cagcaatcag aagcttgaaa ctctggacct
     3721 gggccagaat catttgtgga agagtggcat aattaagctc tttggggttc taagacaaag
     3781 aactggatcc ttgaagatac tcaggttgaa gacctatgaa actaatttgg aaatcaagaa
     3841 gctgttggag gaagtgaaag aaaagaatcc caagctgact attgattgca atgcttccgg
     3901 ggcaacggca cctccgtgct gtgacttttt ttgcctcgag tacccatacg acgtacctga
     3961 ttatgcaact tatccttatg atgtacctga ttatgctagc tatccttatg atgtcccaga
     4021 ctacgcatga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4081 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4141 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4201 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4261 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4321 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4381 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4681 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4741 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4801 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4861 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4921 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4981 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5041 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5101 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5161 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5221 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5281 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5341 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5401 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5461 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5521 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5581 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5641 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5701 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     5761 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     5821 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     5881 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     5941 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6001 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6061 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6121 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6181 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6241 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7141 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     7261 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7321 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7381 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7441 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7501 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7561 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7621 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7681 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7741 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7801 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7861 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7921 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7981 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8041 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8101 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8161 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8221 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8281 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8341 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8401 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     8461 tcgacggatc gggagatctc ccgatcccct atggt
//
