LOCUS V020286 7388 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V020286 VERSION V020286 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 902..2881 /label="MMP2(NM_004530)" /note="MMP2(NM_004530)" /gene="MMP2" CDS 2882..2908 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /transl_table=1 /translation="YPYDVPDYA" /label="HA" polyA_signal 2955..3179 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3225..3653 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 3667..3996 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4063..4857 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5031..5152 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5201..5217) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5225..5241 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5249..5279) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5294..5315 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5603..6188) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6359..7219) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7220..7324) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 catggaggcg ctaatggccc ggggcgcgct cacgggtccc ctgagggcgc tctgtctcct 961 gggctgcctg ctgagccacg ccgccgccgc gccgtcgccc atcatcaagt tccccggcga 1021 tgtcgccccc aaaacggaca aagagttggc agtgcaatac ctgaacacct tctatggctg 1081 ccccaaggag agctgcaacc tgtttgtgct gaaggacaca ctaaagaaga tgcagaagtt 1141 ctttggactg ccccagacag gtgatcttga ccagaatacc atcgagacca tgcggaagcc 1201 acgctgcggc aacccagatg tggccaacta caacttcttc cctcgcaagc ccaagtggga 1261 caagaaccag atcacataca ggatcattgg ctacacacct gatctggacc cagagacagt 1321 ggatgatgcc tttgctcgtg ccttccaagt ctggagcgat gtgaccccac tgcggttttc 1381 tcgaatccat gatggagagg cagacatcat gatcaacttt ggccgctggg agcatggcga 1441 tggatacccc tttgacggta aggacggact cctggctcat gccttcgccc caggcactgg 1501 tgttggggga gactcccatt ttgatgacga tgagctatgg accttgggag aaggccaagt 1561 ggtccgtgtg aagtatggga acgccgatgg ggagtactgc aagttcccct tcttgttcaa 1621 tggcaaggag tacaacagct gcactgatac cggccgcagc gatggcttcc tctggtgctc 1681 caccacctac aactttgaga aggatggcaa gtacggcttc tgtccccatg aagccctgtt 1741 caccatgggc ggcaacgctg aaggacagcc ctgcaagttt ccattccgct tccagggcac 1801 atcctatgac agctgcacca ctgagggccg cacggatggc taccgctggt gcggcaccac 1861 tgaggactac gaccgcgaca agaagtatgg cttctgccct gagaccgcca tgtccactgt 1921 tggtgggaac tcagaaggtg ccccctgtgt cttccccttc actttcctgg gcaacaaata 1981 tgagagctgc accagcgccg gccgcagtga cggaaagatg tggtgtgcga ccacagccaa 2041 ctacgatgat gaccgcaagt ggggcttctg ccctgaccaa gggtacagcc tgttcctcgt 2101 ggcagcccac gagtttggcc acgccatggg gctggagcac tcccaagacc ctggggccct 2161 gatggcaccc atttacacct acaccaagaa cttccgtctg tcccaggatg acatcaaggg 2221 cattcaggag ctctatgggg cctctcctga cattgacctt ggcaccggcc ccacccccac 2281 gctgggccct gtcactcctg agatctgcaa acaggacatt gtatttgatg gcatcgctca 2341 gatccgtggt gagatcttct tcttcaagga ccggttcatt tggcggactg tgacgccacg 2401 tgacaagccc atggggcccc tgctggtggc cacattctgg cctgagctcc cggaaaagat 2461 tgatgcggta tacgaggccc cacaggagga gaaggctgtg ttctttgcag ggaatgaata 2521 ctggatctac tcagccagca ccctggagcg agggtacccc aagccactga ccagcctggg 2581 actgccccct gatgtccagc gagtggatgc cgcctttaac tggagcaaaa acaagaagac 2641 atacatcttt gctggagaca aattctggag atacaatgag gtgaagaaga aaatggatcc 2701 tggcttcccc aagctcatcg cagatgcctg gaatgccatc cccgataacc tggatgccgt 2761 cgtggacctg cagggcggcg gtcacagcta cttcttcaag ggtgcctatt acctgaagct 2821 ggagaaccaa agtctgaaga gcgtgaagtt tggaagcatc aaatccgact ggctaggctg 2881 ctacccatac gacgtaccag attacgcttg actcgagtct agagggcccg tttaaacccg 2941 ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt 3001 gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat 3061 tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag 3121 caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc 3181 ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg 3241 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 3301 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 3361 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 3421 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac 3481 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 3541 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat 3601 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg 3661 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 3721 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 3781 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 3841 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 3901 atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag 3961 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc 4021 attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga 4081 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa 4141 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt 4201 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg 4261 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa 4321 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac 4381 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt 4441 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact 4501 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg 4561 ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg 4621 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc 4681 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt 4741 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc 4801 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg 4861 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg 4921 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct 4981 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta 5041 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 5101 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 5161 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt 5221 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 5281 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 5341 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 5401 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 5461 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 5521 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 5581 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 5641 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 5701 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 5761 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 5821 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 5881 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 5941 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 6001 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct 6061 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 6121 aaaccaccgc tggtagcggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 6181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 6241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 6301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 6361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 6421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 6481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 6541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 6601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 6661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 6721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 6781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 6841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 6901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 6961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 7081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 7141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 7201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 7261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 7321 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc 7381 cctatggt //