LOCUS       V029886                 8782 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V029886
VERSION     V029886
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        202..581
                     /note="human cytomegalovirus immediate early enhancer"
                     /label="CMV enhancer"
     promoter        582..785
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     promoter        830..848
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             931..4302
                     /label="MICAL2(NM_014632)"
                     /note="MICAL2(NM_014632)"
                     /gene="MICAL2"
     polyA_signal    4349..4573
                     /note="bovine growth hormone polyadenylation signal"
                     /label="bGH poly(A) signal"
     rep_origin      4619..5047
                     /direction=RIGHT
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     promoter        5061..5390
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     CDS             5457..6251
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /note="confers resistance to neomycin, kanamycin, and G418
                     (Geneticin®)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /transl_table=1
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
                     /label="NeoR/KanR"
     polyA_signal    6425..6546
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     primer_bind     complement(6595..6611)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    6619..6635
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6643..6673)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6688..6709
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6997..7582)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(7753..8613)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(8614..8718)
                     /gene="bla"
                     /label="AmpR promoter"
ORIGIN
        1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
       61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
      121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      901 cactagtcca gtgtggtgga attcgccacc atgggggaaa acgaggatga gaagcaggcc
      961 caggcggggc aggtttttga gaactttgtc caggcatcca cgtgcaaagg taccctccag
     1021 gccttcaaca ttctcacacg acacctggac ctagaccctc tggaccacag aaacttttat
     1081 tccaagctca agtccaaggt gaccacctgg aaagccaaag ccctgtggta caaattggat
     1141 aagcgtggtt cccacaaaga gtataagcga gggaagtcgt gcacgaacac caagtgtctc
     1201 atagttgggg gaggaccctg tggcttgcgc actgccattg aacttgccta cctgggagcc
     1261 aaagtggtcg tggtggagaa gagggactcc ttctcccgga acaacgtgct acacctctgg
     1321 cctttcacca tccatgacct tcgtggcctg ggagccaaga agttctatgg gaagttctgt
     1381 gctggctcca tcgaccatat cagtattcgc caactacagc tcatcctatt caaggtggcc
     1441 ctgatgctgg gagttgaaat ccatgtgaat gtggagttcg tgaaggttct agagcctcct
     1501 gaagatcaag aaaatcaaaa aattggctgg cgggcagaat ttctccctac agaccattct
     1561 ctgtcggagt ttgagtttga cgtcatcatt ggtgccgatg gccgcaggaa caccctggaa
     1621 gggttcagaa gaaaagaatt ccgtgggaag ctggcgattg ccatcaccgc caacttcata
     1681 aacagaaaca gcacagcgga agccaaggtg gaagagatta gtggtgtggc tttcatcttc
     1741 aatcagaaat tttttcagga ccttaaagaa gaaacaggca tagatcttga gaacattgtt
     1801 tactacaagg actgcaccca ctattttgta atgacagcca agaagcagag cctgctcgac
     1861 aaaggtgtca tcattaacga ctacatcgac acagagatgc tgctgtgtgc ggagaacgtg
     1921 aaccaagaca acctgctatc ctatgcccgg gaagctgcag actttgccac caactaccag
     1981 ctgccatcct tagactttgc catgaaccac tatgggcagc ctgatgtggc catgtttgac
     2041 tttacctgca tgtatgcctc agagaacgcg gccctggtgc gggagcggca ggcgcaccag
     2101 ctgctcgtgg cccttgtggg tgacagcttg cttgagccat tttggcccat gggtacaggc
     2161 tgtgcccgtg gcttcctggc agcctttgac acggcatgga tggtgaagag ctggaaccag
     2221 ggcacccctc ccctggagct gctggctgaa agggaaagtc tctaccggct gttacctcag
     2281 acaaccccgg agaacatcaa caagaacttt gagcagtaca cgttggaccc agggacacgg
     2341 tacccaaacc tcaactcaca ctgtgtcagg ccccatcagg tgaagcattt gtatatcact
     2401 aaggagctgg agcactaccc tctcgagaga ctgggctcgg tgaggagatc tgtcaacctc
     2461 tccaggaagg agtcagatat ccggcccagc aagctcctga cctggtgcca gcagcagaca
     2521 gagggctacc agcatgtcaa cgtcaccgac ctgaccacat cctggcgcag tgggttggcc
     2581 ctgtgtgcca tcatccaccg cttccggcct gagctcatca actttgactc tttgaatgaa
     2641 gatgatgctg tggagaacaa ccagctcgca tttgatgtgg ccgagcgaga gtttgggatc
     2701 cctccagtga ccacgggcaa agagatggca tctgcccagg agcctgacaa gctcagcatg
     2761 gtcatgtacc tctccaagtt ctacgagctc ttccggggca ccccactgag gcccgtggat
     2821 tcttggcgca aaaactatgg agaaaatgct gacctcagct tggccaaatc atccatttct
     2881 aataactatc tcaacctcac atttccaagg aagaggactc cacgggtgga tggtcaaacc
     2941 ggagagaatg acatgaacaa acggagacgg aagggcttca ccaacctgga cgagccttca
     3001 aacttttcca gccgtagctt gggctccaat caagagtgtg ggagcagtaa ggaaggtgga
     3061 aatcagaaca aagtcaagtc catggcgaat cagctgctgg ccaagtttga ggagagcact
     3121 cggaacccct cactcatgaa gcaggaacgc cgtgtctcag ggataggtaa gccggtcctg
     3181 tgctcttcct ccggccctcc tgttcactct tgctgcccca agccggagga ggccacaccc
     3241 agcccatcac ctcctctgaa aaggcagttc ccctctgtgg tcgtgacggg gcacgtgctc
     3301 agagagctca agcaagtgtc tgctggcagt gagtgcctga gcagaccttg gagagccaga
     3361 gccaagtctg acctacagct gggtgggaca gaaaatttcg ctaccctgcc ttctacccgc
     3421 ccgagggcgc aggctctttc cggggtgctg tggcggctgc agcaagtgga ggaaaagatt
     3481 ctccagaaga gggctcagaa cttggccaac agggaatttc acacaaagaa cattaaggag
     3541 aaggcggctc accttgcctc catgtttgga cacggggatt tcccgcagaa taaactactc
     3601 tctaaaggcc tgtctcatac tcatcctcca tctcctccct ctcgccttcc gtctcctgat
     3661 ccagctgctt cttcctctcc atcaactgtt gactctgctt ctcctgccag aaaggaaaag
     3721 aagtcacctt cagggttcca ttttcatccc agccatttga gaacagtgca tcctcagctg
     3781 acggtaggga aagtgtccag cggaataggg gctgcagctg aagtcctggt caatctgtac
     3841 atgaatgatc acagacctaa ggcccaggcc acctctccag acctggaatc tatgcgaaag
     3901 tcatttcccc ttaacctggg aggcagcgac acgtgttact tctgtaagaa acgtgtgtac
     3961 gtgatggaac ggctgagcgc cgagggccac ttcttccacc gggagtgttt ccgctgcagc
     4021 atctgtgcca ccaccttgcg cctggccgcc tacacctttg actgcgatga aggcaaattt
     4081 tactgcaagc ctcacttcat tcactgtaaa accaatagca aacaacggaa gagacgggca
     4141 gagttgaagc aacaaagaga ggaggaggca acatggcaag agcaggaagc ccctcggaga
     4201 gacactccca ccgaaagttc ttgcgcagtg gccgccattg gcaccctgga aggcagcccc
     4261 ccagttcatt tcagccttcc agtgctacac ccacttcttg gctgactcga gtctagaggg
     4321 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4381 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4441 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4501 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     4561 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     4621 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4681 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4741 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4801 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4861 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4921 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4981 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5041 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     5101 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     5161 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     5221 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     5281 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     5341 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     5401 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     5461 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     5521 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     5581 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     5641 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     5701 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     5761 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     5821 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     5881 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     5941 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     6001 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     6061 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     6121 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     6181 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     6241 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     6301 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     6361 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     6421 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     6481 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     6541 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     6601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     6661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     6721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     6781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     6841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7501 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     7561 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7621 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7681 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7741 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7801 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7861 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7921 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     7981 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8041 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8101 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8161 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8221 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8281 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8341 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8401 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8461 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8521 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8581 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8641 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8701 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg
     8761 agatctcccg atcccctatg gt
//
