LOCUS V027036 6487 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V027036 VERSION V027036 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 202..581 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 582..785 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 830..848 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 931..2007 /label="MGME1(NM_001310338)" /note="MGME1(NM_001310338)" /gene="MGME1" polyA_signal 2054..2278 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 2324..2752 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 2766..3095 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 3162..3956 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 4130..4251 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(4300..4316) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 4324..4340 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(4348..4378) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 4393..4414 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(4702..5287) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(5458..6318) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(6319..6423) /gene="bla" /label="AmpR promoter" ORIGIN 1 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt 61 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct 121 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg 181 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta 241 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg 301 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc 361 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa 421 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca 481 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta 541 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt 601 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg 661 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca 721 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca 781 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc 841 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc 901 cactagtcca gtgtggtgga attcgccacc atgaagatga agttatttca gaccatttgc 961 aggcagctca ggagttcaaa gttttctgtg gaatcagctg cccttgtggc tttctctact 1021 tcctcttact catgtggccg gaagaaaaaa gtgaacccat atgaagaagt ggaccaagaa 1081 aaatactcta atttagttca gtctgtcttg tcatccagag gcgtcgccca gaccccggga 1141 tcggtggagg aagatgcttt gctctgtgga cccgtgagca agcataagct gccaaaccaa 1201 ggtgaggaca gacgagtgcc acaaaactgg tttcctatct tcaatccaga gagaagtgat 1261 aaaccaaatg caagtgatcc ttcagttcct ttgaaaatcc ccttgcaaag gaatgtgata 1321 ccaagtgtga cccgagtcct tcagcagacc atgacaaaac aacaggtttt cttgttggag 1381 aggtggaaac agcggatgat tctggaactg ggagaagatg gctttaaaga atacacttca 1441 agttttcatg tttgtgatca tgtgtatatg aagaacctag ccagggacgt ctttttacaa 1501 gggaaacggt tccacgaagc cttggaaagc atactttcac cccaggaaac cttaaaagag 1561 agagatgaaa atctcctcaa gtctggttac attgaaagtg tccagcatat tctgaaagat 1621 gtcagtggag tgcgagctct tgaaagtgct gttcaacatg aaaccttaaa ctatataggt 1681 ctgctggact gtgtggctga gtatcagggc aagctctgtg tgattgattg gaagacatca 1741 gagaaaccaa agccttttat tcaaagtaca tttgacaacc cactgcaagt tgtggcatac 1801 atgggtgcca tgaaccatga taccaactac agctttcagg ttcaatgtgg cttaattgtg 1861 gtggcctaca aagatggatc acctgcccac ccacatttca tggatgcaga gctctgttcc 1921 cagtactgga ccaagtggct tcttcgacta gaagaatata cggaaaagaa aaagaaccag 1981 aatattcaga aaccagaata ttcagaatag ctcgagtcta gagggcccgt ttaaacccgc 2041 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 2101 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 2161 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 2221 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 2281 tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc 2341 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc 2401 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc 2461 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc 2521 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg 2581 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact 2641 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt 2701 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt 2761 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc 2821 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag 2881 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc 2941 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 3001 ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt 3061 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca 3121 ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat 3181 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac 3241 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc 3301 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc 3361 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag 3421 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc 3481 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg 3541 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc 3601 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc 3661 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga 3721 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca 3781 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg 3841 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg 3901 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg 3961 gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga 4021 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg 4081 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat 4141 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 4201 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 4261 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 4321 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 4381 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 4441 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 4501 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4561 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4621 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4681 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4741 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4801 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4861 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4921 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 4981 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5041 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5101 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5161 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5221 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 5281 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 5341 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 5401 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 5461 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 5521 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 5581 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 5641 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 5701 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 5761 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 5821 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 5881 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 5941 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 6001 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 6061 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 6121 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 6181 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 6241 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 6301 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 6361 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 6421 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc 6481 ctatggt //