LOCUS pcDNA3.1-MFN2(human) 7747 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V023070 VERSION V023070 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 235..614 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 615..818 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" promoter 863..881 /note="promoter for bacteriophage T7 RNA polymerase" /label="T7 promoter" CDS 964..3234 /label="MFN2(NM_014874)" /note="MFN2(NM_014874)" /gene="MFN2" CDS 3244..3309 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 3347..3571 /note="bovine growth hormone polyadenylation signal" /label="bGH poly(A) signal" rep_origin 3617..4045 /direction=RIGHT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4059..4388 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4455..5249 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5423..5544 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" primer_bind complement(5593..5609) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" protein_bind 5617..5633 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" promoter complement(5641..5671) /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 5686..5707 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" rep_origin complement(5995..6580) /direction=LEFT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(6751..7611) /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" promoter complement(7612..7716) /gene="bla" /label="AmpR promoter" ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagcttggta ccgagctcgg atccactagt ccagtgtggt ggaattcgcc 961 accatgtccc tgctcttctc tcgatgcaac tctatcgtca cagtcaagaa aaataagaga 1021 cacatggctg aggtgaatgc atccccactt aagcactttg tcactgccaa gaagaagatc 1081 aatggcattt ttgagcagct gggggcctac atccaggaga gcgccacctt ccttgaagac 1141 acgtacagga atgcagaact ggaccccgtt accacagaag aacaggttct ggacgtcaaa 1201 ggttacctat ccaaagtgag aggcatcagt gaggtgctgg ctcggaggca catgaaagtg 1261 gctttttttg gccggacgag caatgggaag agcaccgtga tcaatgccat gctctgggac 1321 aaagttctgc cctctgggat tggccacacc accaattgct tcctgcgggt agagggcaca 1381 gatggccatg aggcctttct ccttaccgag ggctcagagg aaaagaggag tgccaagact 1441 gtgaaccagc tggcccatgc cctccaccag gacaagcagc tccatgccgg cagcctagtg 1501 agtgtgatgt ggcccaactc taagtgccca cttctgaagg atgacctcgt tttgatggac 1561 agccctggta ttgatgtcac cacagagctg gacagctgga ttgacaagtt ttgtctggat 1621 gctgatgtgt ttgtgctggt ggccaactca gagtccaccc tgatgcagac ggaaaagcac 1681 ttcttccaca aggtgagtga gcgtctctcc cggccaaaca tcttcatcct gaacaaccgc 1741 tgggatgcat ctgcctcaga gcccgagtac atggaggagg tgcggcggca gcacatggag 1801 cgttgtacca gcttcctggt ggatgagctg ggcgtggtgg atcgatccca ggccggggac 1861 cgcatcttct ttgtgtctgc taaggaggtg ctcaacgcca ggattcagaa agcccagggc 1921 atgcctgaag gagggggcgc tctcgcagaa ggctttcaag tgaggatgtt tgagtttcag 1981 aattttgaga ggagatttga ggagtgcatc tcccagtctg cagtgaagac caagtttgag 2041 cagcacacgg tccgggccaa gcagattgca gaggcggttc gactcatcat ggactccctg 2101 cacatggcgg ctcgggagca gcaggtttac tgcgaggaaa tgcgtgaaga gcggcaagac 2161 cgactgaaat ttattgacaa acagctggag ctcttggctc aagactataa gctgcgaatt 2221 aagcagatta cggaggaagt ggagaggcag gtgtcgactg caatggccga ggagatcagg 2281 cgcctctctg tactggtgga cgattaccag atggacttcc acccttctcc agtagtcctc 2341 aaggtttata agaatgagct gcaccgccac atagaggaag gactgggtcg aaacatgtct 2401 gaccgctgct ccacggccat caccaactcc ctgcagacca tgcagcagga catgatagat 2461 ggcttgaaac ccctccttcc tgtgtctgtg cggagtcaga tagacatgct ggtcccacgc 2521 cagtgcttct ccctcaacta tgacctaaac tgtgacaagc tgtgtgctga cttccaggaa 2581 gacattgagt tccatttctc tctcggatgg accatgctgg tgaataggtt cctgggcccc 2641 aagaacagcc gtcgggcctt gatgggctac aatgaccagg tccagcgtcc catccctctg 2701 acgccagcca accccagcat gcccccactg ccacagggct cgctcaccca ggaggagttc 2761 atggtttcca tggttaccgg cctggcctcc ttgacatcca ggacctccat gggcattctt 2821 gttgttggag gagtggtgtg gaaggcagtg ggctggcggc tcattgccct ctcctttggg 2881 ctctatggcc tcctctacgt ctatgagcgt ctgacctgga ccaccaaggc caaggagagg 2941 gccttcaagc gccagtttgt ggagcatgcc agcgagaagc tgcagcttgt catcagctac 3001 actggctcca actgcagcca ccaagtccag caggaactgt ctgggacctt tgctcatctg 3061 tgtcagcaag ttgacgtcac ccgggagaac ctggagcagg aaattgccgc catgaacaag 3121 aaaattgagg ttcttgactc acttcagagc aaagcaaagc tgctcaggaa taaagccggt 3181 tggttggaca gtgagctcaa catgttcaca caccagtacc tgcagcccag cagatagctc 3241 gaggactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac 3301 gatgacaagt agtgagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt 3361 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact 3421 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat 3481 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc 3541 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc 3601 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 3661 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 3721 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 3781 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 3841 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 3901 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 3961 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 4021 atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa 4081 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa 4141 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca 4201 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca 4261 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg 4321 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct 4381 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat 4441 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 4501 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 4561 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 4621 ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 4681 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 4741 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 4801 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 4861 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 4921 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 4981 cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 5041 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 5101 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 5161 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 5221 tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca 5281 agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt 5341 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat 5401 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag 5461 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 5521 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt 5581 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 5641 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 5701 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 5761 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 5821 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 5881 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 5941 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 6001 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 6061 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 6121 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 6181 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 6241 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 6301 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 6361 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 6421 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 6481 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtttttttgt 6541 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc 6601 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt 6661 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta 6721 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat 6781 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac 6841 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg 6901 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag 6961 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt 7021 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt 7081 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt 7141 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt 7201 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct 7261 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt 7321 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac 7381 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa 7441 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa 7501 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca 7561 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct 7621 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga 7681 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc 7741 tgacgtc //